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SN/T 3848-2014: Determination method of tea polyphenol residues in foods for export. HPLC method
Status: Valid
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Determination method of tea polyphenol residues in foods for export. HPLC method
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SN/T 3848-2014
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Basic data | Standard ID | SN/T 3848-2014 (SN/T3848-2014) | | Description (Translated English) | Determination method of tea polyphenol residues in foods for export. HPLC method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 17,193 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine | | Issuing agency(ies) | General Administration of Customs | | Summary | This Standard specifies the HPLC method food exports polyphenols. This Standard applies to edible oil, cakes, convenience rice, fried foods and meat sausage tables epigallocatechin gallate (EGCG), epigallocatechin table (EGC), epicatechin gallate (ECG) an | SN/T 3848-2014: Determination method of tea polyphenol residues in foods for export. HPLC method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination method of tea polyphenol residues in foods for export. HPLC method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Detection in food for export polyphenols
High Performance Liquid Chromatography
HPLCmethod
Issued on. 2014-04-09
2014-08-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Entry-Exit Inspection and Quarantine Bureau, Dongguan Comprehensive Technology Center, the People's Republic of China Beijing
Entry Inspection and Quarantine Bureau responsible for drafting.
The main drafters of this standard. Fudeng Zhou, Li Xiaolin, Li Ju, Huang, Yang Xuejiao, Wen Jianchang, Wang Wei, Zhangjiang Feng.
Detection in food for export polyphenols
High Performance Liquid Chromatography
1 Scope
This standard specifies the HPLC method for the export of food polyphenols.
This standard applies to edible oil, cakes, convenience rice, fried foods and meat sausage tables epigallocatechin gallate (EGCG),
Table epigallocatechin (EGC), epicatechin gallate (ECG), and epicatechin (EC) determination does not apply to tea and tea products.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
Edible oil extracted with methanol, freezing, centrifugation and other samples with n-hexane extraction, the extract was frozen, after centrifugation, HPLC
Cytometry, external standard.
4 Reagents and materials
Unless otherwise specified, all reagents were of analytical grade water as a water GB/T 6682 regulations.
4.1 acetonitrile. HPLC grade.
4.2 Methanol. HPLC grade.
4.3 formic acid. HPLC grade.
4.4 epigallocatechin gallate standard (EGCG). CAS No. 989-51-5, molecular formula C22H18O11, molecular weight
458.37, purity of greater than or equal to 95%.
Table 4.5 Standard epigallocatechin (EGC). CAS No. 970-74-1, molecular formula C15H14O7, molecular weight 306.27, purity large
Than or equal to 95%.
4.6 epigallocatechin gallate standard (ECG). CAS No. 1257-08-5, Formula C15H18O10, molecular weight 442.37,
92% or greater purity.
4.7 Standard epicatechin (EC). CAS No. 490-46-0, molecular formula C15H14O6, molecular weight 290.27, purity greater than or equal
At 97%.
4.8 polyphenol component standard stock solution. accurately weighed amount of EGCG, EGC, ECG, EC standards (accurate to 0.0001g), with
Dissolved in methanol and set the volume to 10mL, that was EGCG, EGC, ECG, EC concentrations of standard solution 0.5mg/mL of, 0 ℃ ~
At 4 ℃ preservation, valid for two weeks.
4.9 polyphenol components were mixed standard working solution. Apply the above components polyphenols standard stock solutions were necessary, appropriate diluted with water
When mixed standard working solution concentration using now.
4.10 microporous membrane. 0.45μm, organic phase.
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