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SN/T 3843-2014: Determination of monascus pigment in foods for export
Status: Valid
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| SN/T 3843-2014 | English | 779 |
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Determination of monascus pigment in foods for export
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SN/T 3843-2014
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Basic data | Standard ID | SN/T 3843-2014 (SN/T3843-2014) | | Description (Translated English) | Determination of monascus pigment in foods for export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 30,353 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine | | Issuing agency(ies) | General Administration of Customs | | Summary | This Standard specifies the export of food in monascorubrin amine, monascorubrin cable, cable red yeast, red yeast flavin liquid chromatography - mass spectrometry/mass spectrometry methods. Also it provides high performance liquid chromatography detectio | SN/T 3843-2014: Determination of monascus pigment in foods for export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of monascus pigment in foods for export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of food for export Monascus Pigment
Issued on. 2014-01-13
2014-08-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Jilin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Ma Shumin, glory be, Zhang Xun, Mou Jun, ZHANG Dai Hui, Handa Chuan, Hu Tingting, Kangming Qin.
Determination of food for export Monascus Pigment
1 Scope
This standard provides the food for export Monascus amine, monascorubrin, pigment red yeast, red yeast flavin liquid chromatography - mass spectrometry/mass spectrometry methods.
Also it provides a HPLC method for the export of food in Monascus amine, Monascus Pigment.
This standard applies to the preparation of food for export wine, ice cream, biscuits, ham, red yeast fermented bean curd in red amine, monascorubrin, pigment red yeast, red yeast
Determination of flavin and confirmation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 6682 analytical laboratory use specifications and test methods
3 Liquid chromatography - mass spectrometry/mass spectrometry
3.1 Method summary
Ultrasonic liquid sample water extraction volume after centrifugation, solid and semi-solid samples were dissolved in water to volume, and then extract by solid phase extraction column
Purification, after the sample solution by elution volume for liquid chromatography - mass spectrometry/mass spectrometer and validation, external standard.
3.2 Reagents and materials
3.2.1 Reagents and material requirements
The method used reagents were HPLC grade water GB/T 6682 provisions of distilled water.
3.2.2 Reagents and materials
Reagents and materials used in the method are as follows.
--- Methanol. HPLC grade;
--- Formic acid. chromatography;
--- Acetonitrile. chromatography;
--- Standard substance. Monascus amine (Fmoc-8-AOC-OH, CASN O.126631-93-4, C23H27NO4), monascorubrin
(Monascusred, CASN O.874807-57-5, C23H26O5), Monascus pigment (monascin, CASN O.21516-68-7,
C21H26O5), red yeast flavin (ankaflavin, CASN O.50980-32-0, C23H30O5) purity of greater than or equal to 97%;
--- SPE. C18,1.0g, 6mL, or equivalent person. Before use 5mL methanol, 5mL water-activated;
--- TF microporous membrane. 0.20μm.
3.2.3 preparation of 0.1% aqueous formic acid
1mL acid dissolved in 1000mL water.
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