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LY/T 3208-2020: Guidelines for the conduct of tests for distinctness, uniformity and stability (DUS) - Hawthorn (Crataegus L.)
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Guidelines for the conduct of tests for distinctness, uniformity and stability (DUS) - Hawthorn (Crataegus L.)
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LY/T 3208-2020
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Basic data | Standard ID | LY/T 3208-2020 (LY/T3208-2020) | | Description (Translated English) | Guidelines for the conduct of tests for distinctness, uniformity and stability (DUS) - Hawthorn (Crataegus L.) | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B61 | | Classification of International Standard | 65.020 | | Word Count Estimation | 22,251 | | Date of Issue | 2020-03-30 | | Date of Implementation | 2020-10-01 | | Regulation (derived from) | Announcement No. 6 of 2020 by the State Forestry and Grassland Bureau | | Issuing agency(ies) | State Forestry and Grassland Administration | LY/T 3208-2020: Guidelines for the conduct of tests for distinctness, uniformity and stability (DUS) - Hawthorn (Crataegus L.)
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Guidelines for the conduct of tests for distinctness, uniformity and
stability-Sweetgum (Liquidambar Linn.)
ICS 65.020
B61
LY
People's Republic of China Forestry Industry Standard
LY ／T 3208-2020
Specificity, consistency and stability of new plant varieties
Test Guide Crataegus
2020-10-01 Implementation
Issued by the State Forestry and Grassland Administration
Table of contents
Preface...3
1 Scope...4
2 Normative references...4
3 Terms and definitions...4
4 Technical requirements for DUS testing...4
5 Evaluation of specificity, consistency and stability...6
6 Variety grouping...6
7 Description of trait types and related symbols...7
Appendix A...9
Appendix B...22
References...24
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that certain contents of this standard may involve patents. The issuing agency of this document is not responsible for identifying these patents.
This standard is under the jurisdiction of the State Forestry and Grassland Administration.
This standard corresponds to UPOV Guide TG/239/1, and the degree of consistency with TG/239/1 is not equivalent.
Compared with UPOV guideline TG/239/1, this standard has technical differences, the main differences are as follows.
--Added "Annual branches. internode length", "Annual branches. color", "Leaf. Shape", "Leaf. Upper
Surface main color", "Flower. number of florets per inflorescence", "Flower. number of petals (double-petal flower)", "Flower. multiple color of petals", "Flower.
"Petal folds", "Flower. Anther color", "Fruit. Number of fruits per inflorescence", "Fruit. pulp texture", "Fruit. fruit
Flesh flavor", "Fruit. Fruit point color", "Fruit. Sepal shape", "Fruit. Sepal shape" and "Seed core. hardness"
Wait 16 personality traits.
- Adjusted "Plant. Number of Main Branches", "Plant. Main Branch Extension Posture", "Plant. Crown Shape", "Leaf. Leaf"
Edge serration", "Leaf. Multicolor", "Fruit. Peel Color", "Fruit. Shape", "Fruit. Stem Base Shape" and "Fruit
Reality. 9 personality traits such as main color of pulp.
-Deleted "Leaf. Leaf Density", "Branches. Number of Branches and Spines", "Branches. Length of Branches and Spines", and "Branches. Length
Degree", "Flower. Calyx length", "Flower. Anther shape", "Flower. base color of filament", "Flower. petal posture", "Fruit
Fruit. surface texture, "fruit. fragrance" and "fruit. depth of calyx depression" and other 11 personality traits.
The organization responsible for drafting this standard. Beijing Forestry University
The main drafters of this standard. Lv Yingmin, Ma Suliya, Dong Wenxuan, Zhao Yuhui, Zhang Rui, Huang Xianxing
Guidelines for Testing the Specificity, Consistency and Stability of New Plant Varieties Hawthorn
1 Scope
This standard specifies the technical requirements for the specificity, consistency, and stability testing of new varieties of Crataegus L. plants in the Rosaceae
begging.
This standard applies to the testing of all new species of Hawthorn.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies
In this document. For undated references, the latest version (including all amendments) applies to this document.
GB/T 19557.1-2004 General Principles for Testing the Specificity, Consistency and Stability of New Plant Varieties
3 Terms and definitions
The terms and definitions established in GB/T 19557.1-2004 apply to this document.
4 Technical requirements for DUS testing
4.1 Test materials
4.1.1 The applicant submits plant materials of tested varieties that meet the quantity and quality requirements at the specified time and place. From non-testing country
Applicants should provide relevant documents such as customs and plant quarantine in accordance with relevant regulations on entry, exit and transportation.
Pieces.
4.1.2 The test materials should be plants that have been vegetatively propagated for more than 2 years, and the grafted seedlings should indicate the rootstock.
4.1.3 The number of submitted test materials should not be less than 10 plants.
4.1.4 The test materials submitted should be robust, free from pests and diseases, and free from virus infection.
4.1.5 Except as permitted or required by the approval agency to process the test materials, the submitted test materials shall not be subject to any impact
Treatment of normal expression of traits. If processed, details of the processing should be provided.
4.1.6 Applicants should submit a technical questionnaire when applying, see Appendix B.
4.2 Test method
4.2.1 Test cycle
In the case of meeting the test conditions, at least two growth cycles are tested.
4.2.2 Test location
The test should be carried out in a designated testing organization.
4.2.3 Test conditions
The test should be carried out under the condition that the relevant characteristics of the test material can be fully expressed, and the selected test material should be at least
The site is planted for one year.
4.2.4 Test design
4.2.4.1 The test materials, standard varieties and similar varieties should be planted in the same location and environmental conditions.
4.2.4.2 If certain parts of the plant need to be extracted as samples for the test, the sample collection shall not affect the entire growth cycle of the test plant
Of observations.
4.2.4.3 The breeding methods and management methods of the applied varieties and standard varieties and similar varieties shall be consistent;
And management methods should be consistent.
4.2.4.4 Unless otherwise stated, all observations should be made on at least 9 plants or materials taken from the same part of at least 9 plants
get on.
4.2.4.5 Unless otherwise specified, the number of plants for individual observation traits (VS, MS) shall not be less than 9 plants. Observing plant organs or
For the location, the number of samples for each plant is not less than 2.Group observation traits (VG, MG) should observe the entire community or regulation
Mixed sample of fixed size.
4.2.5 Test methods for similar traits
4.2.5.1 Plants. Except for special instructions, tests should be carried out after the leaves are unfolded in spring.
4.2.5.2 Annual branches. unless otherwise specified, the ones on the sun-facing side of the central crown of the test plant should be selected from after leaf fall to before germination.
Annual branches are tested.
4.2.5.3 Leaves. Unless otherwise specified, the mature leaves of the middle section of the vegetative branches of the current year should be selected for testing in summer.
4.2.5.4 Flower. Unless otherwise specified, the middle branch of the test plant should be selected after the blooming period.
The inflorescence (small flowers in the inflorescence are healthy and open on the same day) for testing.
4.2.5.6 Fruit. Unless otherwise specified, when the fruit is fully mature, the middle part of the sun-facing surface of the outer crown of the test plant should be selected.
The fruit of the fruit branch is tested.
4.2.5.7 Seed kernels. unless otherwise specified, when the fruit is fully mature, select the middle knots on the sun-facing surface of the central part of the test plant.
The fruit seeds of the fruit branches are tested.
4.2.6 Observation and evaluation of color traits
The observation of color characteristics is based on the RHS colour Chart published by the Royal Horticultural Society (RHS).
4.2.7 Test methods for individual traits
4.2.8 Additional testing
For new hybrid varieties obtained through natural or artificial pollination, if the stability test is in doubt, the parent should be added
Specificity, consistency and stability testing.
5 Evaluation of specificity, consistency and stability
5.1 Specificity
If the difference in traits is constant and significant, it is considered specific.
5.1.1 Constant difference
If the difference between the species to be tested and similar species is very significant, only one growth cycle is required. In some cases due to
When the impact of environmental factors makes the difference between the species to be tested and similar species insignificant, at least two growth cycles are required.
test.
5.1.2 Significant difference
5.1.2.1 Specific evaluation of quality traits
As long as there is a difference between a test variety and a similar variety, it can be determined that the variety has specificity.
5.1.2.2 Specific evaluation of quantitative traits
The difference between the test variety and the two discontinuous codes that have at least one trait with the similar variety can determine that the variety has
Specificity.
5.1.2.3 Specific evaluation of false quality traits
There is a difference in at least one trait between the test variety and a similar variety, or the difference between two discrete codes of a trait,
It can be determined that the variety has specificity.
5.2 Consistency
The consistency is judged by the heterotypic method. According to the 1% population standard and 95% reliability probability, the most heterotypic plant among the 10 observed plants
The maximum allowable value is 1.
5.3 Stability
If the variety to be tested meets the requirements for specificity and consistency, the variety can be considered stable.
In special circumstances or when there are doubts, it is necessary to pass the test again for a growth cycle, or the applicant provides new test materials,
Test whether it expresses the same characteristics as the previously provided test materials.
6 Variety grouping
6.1 Variety group description
Determine the grouping situation of the tested varieties according to the grouping traits, and select similar varieties to be included in the specific growth test
6.2 Group traits
6.2.1 Plants. number of main stems (character 1 in Table A.1).
6.2.2 Plant. Main branch extension posture (character 2 in Table A.1).
6.2.3 Leaf. Leaf split (character 13 in Table A.1).
6.2.4 Flower. type (character 24 in Table A.1).
6.2.5 Flower. main color of petals (character 29 in Table A.1).
6.2.6 Fruit. peel color (character 38 in Table A.1).
7 Description of trait types and related symbols
7.1 Types of traits
7.1.1 Asterisk traits (see the traits marked with "*" in Table A.1). refer to the use of new varieties for the purpose of harmonizing and unified characterization during review
When conducting DUS test, all'star traits' should be tested.
7.1.2 Plus sign traits (see the traits marked as "" in Table A.1). refer to the traits (A.2) that are illustrated in Table A.1.
7.2 Character expression status and codes
7.3 Expression types
Character expression types provided by GB/T 19557.1-2004.qualitative traits (QL), quantitative traits (QN) and false qualitative
State (PQ).
7.4 Standard varieties
A variety used to accurately and vividly demonstrate the expression status of a certain trait (especially a quantitative trait).
7.5 Symbol description
Appendix A
(Normative appendix)
Variety traits
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