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LY/T 3206-2020: Guidelines for the conduct of tests for distinctness, uniformity and stability(DUS) - Bougainvillea (Bougainvillea Comm. ex Juss)
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Guidelines for the conduct of tests for distinctness, uniformity and stability(DUS) - Bougainvillea (Bougainvillea Comm. ex Juss)
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LY/T 3206-2020
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Basic data | Standard ID | LY/T 3206-2020 (LY/T3206-2020) | | Description (Translated English) | Guidelines for the conduct of tests for distinctness, uniformity and stability(DUS) - Bougainvillea (Bougainvillea Comm. ex Juss) | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B61 | | Classification of International Standard | 65.020 | | Word Count Estimation | 26,265 | | Date of Issue | 2020-03-30 | | Date of Implementation | 2020-10-01 | | Regulation (derived from) | Announcement No. 6 of 2020 by the State Forestry and Grassland Bureau | | Issuing agency(ies) | State Forestry and Grassland Administration | LY/T 3206-2020: Guidelines for the conduct of tests for distinctness, uniformity and stability(DUS) - Bougainvillea (Bougainvillea Comm. ex Juss)
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Guidelines for the Conduct of Tests for Distinctness, Uniformity and
Stability-Bougainvillea (Bougainvillea Comm.ex Juss)
ICS 65.020
B 61
LY
People's Republic of China Forestry Industry Standard
Specificity, consistency and stability of new plant varieties
Test Guide Bougainvillea
2020-03-30 release
2020-10-01 Implementation
Issued by the State Forestry and Grassland Administration
Table of contents
Foreword...II
1 Scope...1
2 Normative references...1
3 Terms and definitions...1
4 Technical requirements for DUS testing...1
4.1 Test materials...1
4.2 Test method...1
5 Evaluation of specificity, consistency and stability...2
5.1 Specificity...2
5.2 Consistency...2
5.3 Stability...3
6 Variety grouping...3
6.1 Description of Variety Group...3
6.2 Grouping traits...3
7 Description of trait types and related symbols...3
7.1 Types of traits...3
7.2 Character expression status and codes...3
7.3 Expression types...3
7.4 Standard varieties...3
7.5 Symbol description...3
8 Technical Questionnaire...4
Appendix A (normative appendix) Variety traits...5
Appendix B (informative appendix) Technical questionnaire...18
References...20
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009 and GB/T.19957.1-2004.
Please note that certain contents of this standard may involve patents, and the issuing organization of this standard does not bear the responsibility for identifying these patents.
Appendix A of this standard is a normative appendix, and Appendix B is a normative appendix.
This standard was proposed and managed by the State Forestry and Grassland Administration.
Drafting organizations of this standard. Institute of Forestry, Chinese Academy of Forestry, Institute of Tropical Horticulture, Hainan Academy of Agricultural Sciences.
The main drafters of this standard. Zhang Chuanhong, Yang Jun, Li Dongliang, Fu Ruikan, Yu Xuedan, Yun Yong.
Guidance for testing the specificity, consistency and stability of new plant varieties
1 Scope
This standard specifies the specificity, consistency and stability testing techniques for new varieties of Bougainvillea (Bougainvillea Comm. ex juss) plants.
Technical requirements.
This standard is applicable to the testing of all new varieties of Bougainvillea.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 19557.1 General Principles for Testing the Specificity, Consistency and Stability of New Plant Varieties
3 Terms and definitions
The terms and definitions defined in GB/T 19557.1 apply to this standard.
4 Technical requirements for DUS testing
4.1 Test materials
4.1.1 The variety right applicant shall submit the plant materials of the tested variety that meet the quantity and quality requirements at the specified time and place. From non-testing country
For materials submitted by regions or regions, the applicant shall provide relevant documents such as customs and plant quarantine in accordance with relevant regulations on entry, exit and transportation.
4.1.2 The test materials submitted should be plants that have been propagated by cutting or grafting for at least 1 year. If grafting seedlings, please specify the grafting rootstock.
4.1.3 The number of test materials shall not be less than 12 scions or 12 seedlings.
4.1.4 The test material should be a healthy plant without pests and diseases.
4.1.5 Except for the processing of materials permitted or required by the approval agency, the submitted plant materials should not be subjected to any additional treatment that affects the expression of traits.
deal with. If it has been processed, details of the processing should be provided.
4.2 Test method
4.2.1 Test cycle
If the test conditions are met, at least one growth cycle should be tested.
4.2.2 Test location
The test should be carried out in the test base or laboratory designated by the approval agency.
4.2.3 Test conditions
The test should be carried out under the condition that the relevant traits of the variety to be tested can be fully expressed. The selected test materials should at least be planted at the test site
More than 1 year.
4.2.4 Test design
4.2.4.1 The test variety materials should be planted in the test area with 10 plants, and the standard varieties and similar varieties should be planted in the same location and environmental conditions.
4.2.4.2 If certain parts of the plant need to be extracted as samples for the test, the sample collection shall not affect the observation of the entire growth cycle of the test plant.
4.2.4.3 Unless otherwise stated, all observations should be made on 10 plants or materials taken from the same part of 10 plants.
4.2.5 Test methods for similar traits
4.2.5.1 Plants (Character No. 1 in Appendix A Table A.1)
Before germination in spring, unpruned test plants were selected as test materials for plant traits.
4.2.5.2 Annual branches (character number 3～6 in Appendix A Table A.1)
Before sprouting in spring, select the middle section of the annual shoots on the sunny side of the test plant (one shoot for each plant) as the annual shoots.
Test materials for similar traits.
4.2.5.3 Leaf (Character number 7～10, 12～19 in Table A.1 of Appendix A)
In summer, select the middle 1/3 of the annual branch on the sunny side of the test plant and the fully expanded mature leaves (one leaf for each plant
Sheet) as observational materials for various traits of leaves.
4.2.5.4 Inflorescence (character number 21～24 in Appendix A, Table A.1)
In the blooming period, select the inflorescences on the sun-facing side of the middle of the test plants (one inflorescence per plant) as the test material.
4.2.5.5 Bracts (the serial numbers of traits in Table A.1 in Appendix A are 25-30, 34-41)
In the full bloom period, select the middle bracts of the inflorescence (one bract for each plant) on the sun-facing side of the test plant as the test material.
4.2.6 Individual traits in test methods
4.2.6.1 Young shoots (character number 2 in Appendix A Table A.1)
In the spring, select the middle of the young shoots when the germinating length of the young shoots on the sun-facing side of the test plant is less than 20cm as the test material, and each plant
Pick a young branch.
4.2.6.2 Young leaf color (character number 11 in Table A.1 in Appendix A)
In the spring, the newly sprouting young leaves in the middle of the branches on the sunny side of the test plants are selected as the test materials, and one young leaf is selected from each plant. Correct
For multicolor varieties, only the dominant color of young leaves is observed.
4.2.6.3 Inflorescence placement position (character number 20 in Table A.1 in Appendix A)
In the blooming period, select annual shoots (one shoot for each plant) on the sun-facing side of the test plant as the test material.
4.2.6.4 Persistent bracts (character number 31 in Table A.1 in Appendix A)
15 days after the fading of the flowering period, select the middle bracts of the sun-facing surface of the test plants (one bract for each plant) as the test material
material.
4.2.6.5 Flower sepals (character number 32 in Appendix A Table A.1)
In the blooming period, select the calyx in the middle of the sun-facing surface of the test plant of the single bract type variety (one calyx for each plant)
As a test material.
4.2.6.6 Early budding young bracts (character number 33 in Table A.1 in Appendix A)
In the initial flowering period, select the young bracts (one bract for each plant) newly born on the sunny side of the test plant as the test material.
4.2.7 Observation and evaluation of color traits
The observation of color characteristics should be compared with the samples collected by the Royal Horticultural Society (RHS) according to the sampling methods in 4.2.5 and 4.2.6.
The RHS colour chart is the standard.
4.2.8 Additional testing
If necessary, the traits in Table A.1 or traits not listed in this guide can be used for additional testing.
5 Evaluation of specificity, consistency and stability
5.1 Specificity
If the difference in traits is constant and significant, it is considered specific.
5.1.1 Constant difference
If the difference between the species to be tested and similar species is very clear, only one growth cycle is required. In some cases due to environmental factors
If the difference between the variety to be tested and the similar variety is not clear due to the influence of, at least 2 or more growth cycles are required.
5.1.2 Significant difference
Specificity evaluation of quality traits. As long as there is a difference in one trait between the tested variety and similar varieties, it can be determined that the variety has specificity.
Specific evaluation of quantitative traits. if there are at least two discontinuous codes between the tested variety and similar varieties, it can be determined that the variety has
Specificity.
Evaluation of the specificity of false quality traits. there is a significant difference in at least one trait between the tested variety and the similar variety, or two of the same trait
The difference of discontinuous codes can determine that the variety has specificity.
5.2 Consistency
The consistency judgment adopts the heterogeneous plant method. According to the 1% population standard and 95% reliability probability, the maximum allowable rate of heterotypic plants among 10 observed plants
The allowable value is 1.
5.3 Stability
5.3.1 The applied variety meets the consistency requirements in the test, and the variety can be considered as stable.
5.3.2 In special circumstances or when there are doubts, it is necessary to pass the test for one growth cycle again, or the applicant shall provide new test materials to test
Does it express the same characteristics as the previously provided test materials?
6 Variety grouping
6.1 Variety group description
Determine the grouping situation of the new varieties to be tested according to the grouping characteristics, and select similar varieties to be included in the specific growth test.
6.2 Grouping traits
6.2.1 Leaf. secondary color (see the character number 13 in Table A.1 in Appendix A).
6.2.2 Inflorescence. bract type (see table A.1 character number 22 in Appendix A).
6.2.3 Early bracts. the main color of the inner surface (the sepals are open) (see the character number 35 in Table A.1 in Appendix A).
Group 1.White
Group 2.Yellow
Group 3.Orange
Group 4.Red
Group 5.Pink
Group 6.Fuchsia
Group 7.Purple
Group 8.Blue-purple
7 Description of trait types and related symbols
7.1 Types of traits
7.1.1 Star traits (characters marked with "(*)" in Table A.1 of Appendix A). refer to the use of new varieties for harmonizing the description of traits during review
For important traits of cultivars, all "star traits" should be tested when testing.
7.1.2 Plus traits (the traits marked with “()” in Table A.1 of Appendix A). refer to the graphical description of the traits in Table A.1 of Appendix A
Status (see A.2).
7.2 Character expression status and codes
The description of traits in Table A.1 has clearly given the standard definition of the expression status of each trait, in order to facilitate the description of the expression status of the trait.
The description, analysis and comparison, each expression state has a corresponding digital code.
7.3 Expression types
GB/T 19557.1 provides the expression types of traits. qualitative traits (QL), quantitative traits (QN) and pseudo-qualitative traits (PQ)
Explanation of terms.
7.4 Standard varieties
A variety used to accurately and vividly demonstrate the expression status of a certain trait (especially a quantitative trait).
7.5 Symbol description
8 Technical questionnaire
Variety rights applicants should submit a technical questionnaire when applying, see Appendix B.
Appendix A
(Normative appendix)
Variety traits
A.1 Character table
A.2 Character diagram
A.2.1 Table A.1 No. 1 variety traits (plant. plant type)
A.2.2 Table A.1 No. 3 variety traits (annual branches. internode length)
A.2.3 Characters of Nos. 7, 8, 19 in Table A.1 (leaf. length; leaf. width; petiole. length)
A.2.4 Characters of No. 9 variety in Table A.1 (leaf. shape)
A.2.5 Characters of No. 10 varieties in Table A.1 (leaf. base shape)
A.2.6 In Table A.1, the serial number 11 variety traits (young leaves. color), 12 variety traits (leaf. main color), 13 variety traits (leaf. secondary color),
14 variety traits (leaf. secondary color distribution) and 15 variety traits (leaf. third-level color)
A.2.7 Characters of No. 14 variety in Table A.1 (leaf. secondary color distribution)
A.2.8 Characters of No. 16 variety in Table A.1 (leaf. wavy edge)
A.2.9 Characters of No. 20 variety in Table A.1 (inflorescence. implantation position)
A.2.10 Characters of No. 21 variety in Table A.1 (peduncle. length)
A.2.11 Characters of No. 22 variety in Table A.1 (inflorescence. bract type)
A.2.12 Characters of varieties with serial numbers 23 and 24 in Table A.1 (only for varieties with single bract type. inflorescence. number of bract clusters; inflorescence. bract cluster density)
A.2.13 Characters of varieties with serial numbers 25, 26 and 32 in Table A.1 (Bracts. length; bracts. width; limited to varieties with single bracts. flower
Sepals. upper surface color)
A.2.14 Characters of No. 27 variety in Table A.1 (Bracts. shape)
A.2.15 Characters of No. 28 varieties in Table A.1 (Bracts. Apex shape)
A.2.16 Characters of No. 29 variety in Table A.1 (Bracts. base shape)
A.2.17 Characters of No. 30 variety in Table A.1 (Bracts. Posture)
A.2.18 Characters of No. 33 variety in Table A.1 (early budding young bracts. main color of outer surface)
Note. The main bract color refers to the color occupying the largest area of the bract; the secondary bract color refers to the color occupying the second largest area of the bract; if there are two colors
The area occupied by the colors is almost the same, and the darker color is the dominant color. The third color of bracts refers to the color that occupies the third largest area of bracts.
A.2.19 Characters of No. 39 variety in Table A.1 (initial bracts. distribution position of secondary color on inner surface (flower sepals open))
Note. The main bract color refers to the color occupying the largest area of the bract; the secondary bract color refers to the color occupying the second largest area of the bract; if there are two colors
The area occupied by the colors is almost the same, and the darker color is the dominant color. The third color of bracts refers to the color that occupies the third largest area of bracts.
BA
Appendix B
(Informative appendix)
Technical questionnaire
Number (applicants do not need to fill in)
1.The name of the variety to be registered (please indicate the Chinese name and scientific name)
Breeder number
2.Applicant Information
3.Origin of variety
5.Comparison of similar varieties
Variety names similar to this variety.
Typical differences with similar varieties.
6.Summary of variety traits (detailed description in accordance with the content of the trait table in Appendix Table A1)
7.Additional information (can distinguish traits of varieties, etc.)
7.1 Adversity and adaptability (drought resistance, cold resistance, waterlogging resistance, salt alkali resistance, disease and insect pest resistance, etc. characteristics).
7.2 Key points of reproduction.
7.3 Main points of cultivation management.
7.4 Other information.
8.Test requirements (special conditions required for testing of this variety).
9.Other information that helps to identify the application species.
Note. A4 paper supplementary explanation can be attached when the space reserved for each clause of the above form is insufficient.
Applicant’s Signature. Date. YYYY
[6] Science and Technology Development Center of Ministry of Agriculture, General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of China
Committee. General Guidelines for Testing the Specificity, Consistency and Stability of New Plant Varieties GB/T 19557.1-2004.Beijing. China
National Standard Press,.2004.
[7] Plant New Variety Testing Center of the Ministry of Agriculture, National Standardization Technical Committee for New Plant Varieties. New plant varieties are specific and consistent
General Principles of Sex and Stability Testing and Uniform Description TGP/1/3.Beijing. China Agricultural Technology Press,.2007.
[8] Chen Tao. Bougainvillea. Beijing. China Agriculture Press,.2007.
[9] Zhou Qun. Bougainvillea cultivation and appreciation. Beijing. Golden Shield Publishing House,.2009.
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