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SN/T 2525-2010 English PDF

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SN/T 2525-2010: Detection of Clostridium botulinum in foods by PCR
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Standard similar to SN/T 2525-2010

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Basic data

Standard ID SN/T 2525-2010 (SN/T2525-2010)
Description (Translated English) Detection of Clostridium botulinum in foods by PCR
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 12,138
Date of Issue 2010-03-02
Date of Implementation 2010-09-16
Quoted Standard GB/T 4789.12; GB/T 6682; GB 19489; GB/T 27403; SN/T 1193
Regulation (derived from) National Quality Inspection (2010) 98
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the foods PCR assay for detection of Clostridium botulinum. This standard applies to foods A, B, E, F Detection of Clostridium botulinum.

SN/T 2525-2010: Detection of Clostridium botulinum in foods by PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of Clostridium botulinum in foods by PCR Exit inspection and quarantine industry standard book People's Republic of China PCR detection of Clostridium botulinum in foods Issued on. 2010-03-02 2010-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

The criterion-referenced U.S. FDABAM Chapter 17, Part 5. A, B, E, F PCR method for detection of Clostridium botulinum [U. S. FDA, BacteriologicalAnalyticalManualOnline, Chapter17 (V), 2001. SpecificDetectionofClostridiumbotu- linumTypesA, B, EandFUsingthePolymeraseChainReaction (PCR). ] After the development of research and verification. The Standard Appendix A, Appendix B are normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Xiamen, People's Republic of China Exit Inspection and Quarantine. The main drafters of this standard. Chen Shuangya, Xie star, Zhang Yongxiang, THE STAFF force Wang Qun, Liu Tong, Peng Xiaoli, Zhou like tiger, Zhang Mengzhang. This standard is the first release of the entry-exit inspection and quarantine industry standards. PCR detection of Clostridium botulinum in foods

1 Scope

This standard specifies the method of PCR detection of Clostridium botulinum in foods. This standard applies to food products A, B, E, F detection of Clostridium botulinum.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 4789.12 Microbiological examination of food hygiene Clostridium botulinum and botulinum toxin testing GB/T 6682 Water Analysis Laboratory specifications and test methods GB 19489 General requirements for laboratory biosafety GB/T 27403 laboratory quality control of food molecular biology standardized testing SN/T 1193 genetic testing laboratory technical requirements 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this standard. 3.1 Terms and Definitions 3.1.1 Clostridium botulinum is families Clostridium Gram-positive bacillus, anaerobic, generating a suitable medium and under specific environmental conditions Nerve palsy toxins are highly toxic botulinum toxin. 3.1.2 Template DNA to high temperature variability into a single strand, DNA polymerase and under appropriate reaction conditions, depending on the design template sequence The two primers are complementary sequences with the corresponding period of annealing takes place on both strands of the template DNA combined with each other, followed by DNA polymerase Under the action of DNA in four (dNTP) as substrates, primer annealing can be extended, and then repeated denaturation, annealing and extension This circulation, located in two DNA fragments of known sequences were amplified geometrically. 3.2 Acronyms 3.2.1 Base pairs. 3.2.2 DNA. 3.2.3 Deoxynucleoside triphosphates.

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