SN/T 2521: Evolution and historical versions
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| SN/T 2521-2018 | English | RFQ |
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(Listeria monocytogenes serotyping method)
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SN/T 2521-2018
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| SN/T 2521-2010 | English | 239 |
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Serotyping of Listeria monocytogenes
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SN/T 2521-2010
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PDF similar to SN/T 2521-2018
Basic data | Standard ID | SN/T 2521-2018 (SN/T2521-2018) | | Description (Translated English) | (Listeria monocytogenes serotyping method) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Date of Issue | 2018-03-12 | | Date of Implementation | 2018-10-01 | | Older Standard (superseded by this standard) | SN/T 2521-2010 | | Regulation (derived from) | National Quality Inspection (2018) 76 | | Issuing agency(ies) | General Administration of Customs | SN/T 2521-2010: Serotyping of Listeria monocytogenes---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Serotyping of Listeria monocytogenes
Exit inspection and quarantine industry standard book People's Republic of China
Listeria monocytogenes bacteria serotype method
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Zeng Jing, Wei Haiyan, ZHANG Xi Meng, Wang Jinhua, Chan Kwong-chuen, Zhang Huiyuan, Rao red, Fu Pu Bo, Chang Xiaohui, Zhang Xin,
Wang Qi, Ma Xu.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Listeria monocytogenes bacteria serotype method
1 Scope
This standard specifies monocytogenes Listeria serotyping method.
This standard applies to monocytes Listeria bacteria serotype, monocytes Listeria can refer to traceability
The present method.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 4789.30 Microbiological examination of food hygiene Listeria monocytogenes bacteria test
GB 19489 General requirements for laboratory biosafety
3 equipment and materials
3.2 Refrigerator. 4 ℃ ~ 6 ℃.
3.3 McFarland turbidity meter.
3.4 heater.
3.5 inoculating needle.
3.6 tube. 6mm × 50mm.
3.7 Test tube rack. Suitable for 6mm × 50mm tubes.
3.8 Pipette. Apply to 25μL, 50μL, 100μL.
3.9 constant temperature water bath. 48 ℃.
3.10 sterile slide.
3.11 biosafety cabinet.
4 media and reagents
4.1 SIM power Agar. see Section A. Chapter 1.
4.2 tryptose phosphate broth (TPB). See Section A. 2.
4.3 37% formaldehyde solution.
4.4 0.85% sterile saline. see Section A. 3.
4.5 saline containing 0.5% formaldehyde. see Section A. 4.
4.6 O antisera.
4.7 H antisera.
5 Method summary
Tested strain should be identified confirmed as monocytogenes Listeria pure culture monocytogenes Listeria resistance
Listeria genus and serum cross-reactivity between other kinds of phenomena, so misidentifications or impure cultures, making Serotype
It will draw the wrong results; H antigen, also known as flagellar antigen, H antigen located on the bacterial flagellum, use of sports identification H antigen should be in
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