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Determination of microbiological in cosmetics - Part 8: Candida albicans
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SN/T 2206.8-2013
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Basic data | Standard ID | SN/T 2206.8-2013 (SN/T2206.8-2013) | | Description (Translated English) | Determination of microbiological in cosmetics - Part 8: Candida albicans | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Word Count Estimation | 16,171 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs |
SN/T 2206.8-2013: Determination of microbiological in cosmetics - Part 8: Candida albicans---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Cosmetic microbiological test methods - Part 8. Candida albicans)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Cosmetic microbiological test method
Part 8. Candida albicans
Part 8. Candidaalbicans
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2206 "cosmetic microbiological test method" is divided into 13 parts.
--- Part 1. Salmonella;
--- Part 2. aerobic Bacillus subtilis and Bacillus cereus;
--- Part 3. Klebsiella pneumoniae;
--- Part 4. Streptococcus;
--- Part 5. Enterococcus;
--- Part 6. Clostridium tetani;
--- Part 7. Western blot detection of BSE pathogen;
--- Part 8. Candida albicans;
--- Part 9. bile acid tolerance Gram-negative bacteria;
--- Part 10. Staphylococcus aureus PCR method;
--- Part 11. Staphylococcus aureus multiplex real-time PCR method;
--- Part 12. Pseudomonas aeruginosa PCR method;
--- Part 13. maltophilia Aeromonas.
This section SN/T 2206 Part 8.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute.
The main drafters of this section. Lu Jing Zhang, Wan Zhigang, Zhang Heng, Tangmu Jin, Huang Hua, Liu Huiling Zhao Fang, Hong Koyanagi, Fan put, Ding Jing.
Cosmetic microbiological test method
Part 8. Candida albicans
1 Scope
This section SN/T 2206 specifies the method for the detection of Candida albicans in cosmetics.
This section applies to cosmetics detect Candida albicans.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 7918.1 Cosmetics Standard methods of microbiological General
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Candida albicans Candidaalbicans
Also known as Candida albicans is widely present in a conditioned nature of pathogenic fungi, may also be present in normal human oral cavity, upper respiratory tract,
Intestine and vagina, generally in the normal body in small quantities, do not cause disease, when the body's normal flora or resistance is lowered can cause candidiasis
disease. When tested according to the method specified in this standard, Candida albicans in the selection culture Giza's dextrose agar surface white to pale
Brown, smooth, raised colonies; the main features of the performance of Candida albicans germ tubes can be formed, which can produce false hyphae and chlamydospores.
3.2
Chlamydospores clamydospore
Spores under adverse environmental conditions into the dormant period, mycelium concentrated cytoplasm, cell wall thickening and the formation of a special structure under the microscope
The performance of the top or short side branches mycelium spore wall thickness, strong refraction large spherical spores. Chlamydospores still has the ability to reproduce.
3.3
Germ tube Germtube
From yeast Candida albicans hyphae toward a phase transition in the early form of false mycelium, is based on the identification of Candida albicans. bud
Tube formation is closely related to C. albicans virulence enhanced.
4 instruments, apparatus and standard strain
4.1 water bath. 45 ℃ ± 1 ℃.
Thermometer 4.2. 1 ℃ ~ 55 ℃, sub-scale 0.1 ℃.
4.3 Incubator. 36 ℃ ± 1 ℃, 30 ℃ ± 1 ℃.
4.4 inoculating loop. diameter 3mm.
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