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SN/T 2206.10-2014: Determination of microorganism in cosmetics. Part 10: PCR method for Staphylococcus aureus in cosmetics
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2206.10-2014 | English | 239 |
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Determination of microorganism in cosmetics. Part 10: PCR method for Staphylococcus aureus in cosmetics
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SN/T 2206.10-2014
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Basic data | Standard ID | SN/T 2206.10-2014 (SN/T2206.10-2014) | | Description (Translated English) | Determination of microorganism in cosmetics. Part 10: PCR method for Staphylococcus aureus in cosmetics | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 9,923 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Quoted Standard | GB/T 6682-2008; GB 7918.1; GB 7918.5; SN/T 1193 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the cosmetics Staphylococcus aureus PCR detection method. This standard applies to the detection of Staphylococcus aureus in cosmetics. | SN/T 2206.10-2014: Determination of microorganism in cosmetics. Part 10: PCR method for Staphylococcus aureus in cosmetics
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Determination of microorganism in cosmetics.Part 10. PCR method for Staphylococcus aureus in cosmetics
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Cosmetic microbiological test method
Part 10. Staphylococcus aureus PCR.
Part 10. PCRmethodforStaphylococcusaureusincosmetics
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2206 "cosmetic microbiological test method" is divided into 13 parts.
--- Part 1. Salmonella;
--- Part 2. aerobic Bacillus subtilis and Bacillus cereus;
--- Part 3. Klebsiella pneumoniae;
--- Part 4. Streptococcus;
--- Part 5. Enterococcus;
--- Part 6. Clostridium tetani;
--- Part 7. Western blot detection of BSE pathogen;
--- Part 8. Candida albicans;
--- Part 9. bile acid tolerance Gram-negative bacteria;
--- Part 10. Staphylococcus aureus PCR method;
--- Part 11. Staphylococcus aureus multiplex real-time PCR method;
--- Part 12. Pseudomonas aeruginosa PCR method;
--- Part 13. maltophilia Aeromonas.
This section SN/T 2206 Part 10.
This section drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Shandong Entry Inspection and Quarantine Bureau, People's Republic of China Shanghai Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this section. Paul sea room, Bo, Jiang Yinghui, Liuhui Mei, Li Zhengyi, Lin Yang Jie, Lu Rong, Jia Juntao, Ray quality text, Sun Tao.
Cosmetic microbiological test method
Part 10. Staphylococcus aureus PCR.
1 Scope
This section SN/T 2206 provides for cosmetics Staphylococcus aureus PCR detection method.
This section applies to the detection of Staphylococcus aureus in cosmetics.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682-2008 Analysis
GB 7918.1 General Cosmetics Standard methods of microbiological
GB 7918.5 Microbiological Criteria for cosmetic testing methods Staphylococcus aureus
SN/T 1193 genetic testing laboratory technical requirements
3 Terms, definitions and abbreviations
The following terms, definitions and abbreviations apply to this document.
3.1 Terms and Definitions
3.1.1
Staphylococcus aureus Staphylococcusaureus
Gram-positive bacteria, spherical, grape-like arrangement, no spores, no capsule, can break down mannitol, coagulase positive.
3.2 Acronyms
The following abbreviations apply to this document.
PCR. polymerase chain reaction, referred to as PCR (polymerasechainreaction)
DNA. deoxyribonucleic acid (deoxyribonucleicacid)
dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate)
Taq. Thermus aquaticus bacteria (Thermosaquaticu)
4 Method summary
Cosmetics by enrichment, the enrichment broth or extract the DNA colonies can, to extract the DNA as a template Staphylococcus aureus high
PCR amplification of fragments from agarose gel electrophoresis test whether the PCR products characteristic bands, and thus whether the contamination golden cosmetics
Aureus rapid screening test.
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