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SN/T 2206.6-2010: Determination of microbiological in cosmetics. Part 6: Clostridium tetani
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2206.6-2010 | English | 159 |
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Determination of microbiological in cosmetics. Part 6: Clostridium tetani
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SN/T 2206.6-2010
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Basic data | Standard ID | SN/T 2206.6-2010 (SN/T2206.6-2010) | | Description (Translated English) | Determination of microbiological in cosmetics. Part 6: Clostridium tetani | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 6,612 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Quoted Standard | GB/T 19489; cosmetics hygiene norms (Ministry of Health 2007) | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the cosmetic method for detecting Clostridium tetani. This standard applies to all types of Clostridium tetani in cosmetics testing. | SN/T 2206.6-2010: Determination of microbiological in cosmetics. Part 6: Clostridium tetani---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of microbiological in cosmetics.Part 6. Clostridium tetani
Exit inspection and quarantine industry standard book People's Republic of China
Cosmetic microbiological test method
Part 6. Clostridium tetani
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2206 "cosmetic microbiological test method" is divided into the following sections.
--- Part 1. Salmonella;
--- Part 2. aerobic Bacillus subtilis and Bacillus cereus;
--- Part 3. Klebsiella pneumoniae;
--- Part 4. Streptococcus;
--- Part 5. Enterococcus;
--- Part 6. Clostridium tetani.
This section SN/T 2206 Part 6.
This section of Appendix A normative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China, Shenzhen CIQ.
The main drafters of this section. Fan release, Zhu Hai, Tang little ice, Zhao Fang, Kuangyan Yun.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Cosmetic microbiological test method
Part 6. Clostridium tetani
1 Scope
This section SN/T 2206 specifies the method for the detection of Clostridium tetani in cosmetics.
This section applies to all types of Clostridium tetani cosmetics testing.
2 Normative references
The following documents contain provisions which, through reference SN/T 2206 and become part of the provisions of this section. For dated references,
All subsequent amendments (not including errata content) or revisions do not apply to this section, however, encourage the agreement on this section
Whether the parties can study the latest versions of these documents. For undated reference documents, the latest versions apply to this section.
GB/T 19489 Laboratory Biosafety General Requirements
Hygienic Standard for Cosmetics (Ministry of Health, 2007)
3 Method summary
According Clostridium tetani unique morphology, biological characteristics and cultural characteristics, using anaerobic culture method, application of blood agar plates were isolated,
And produce β-hemolytic ring according to the strain on blood agar around the colony, was migratory growth of Clostridium Gram-positive, catalase-negative and other special
Sign certificates for identification, to mice virulence test results for the final determination of possible cosmetic Clostridium tetani qualitative test.
4 Equipment and Materials
4.1 Incubator. 36 ℃ ± 1 ℃.
4.2 microscope.
4.3 anaerobic culture apparatus.
4.4 Balance. Range 1kg, a sense of the amount of 0.1g.
4.5 Sterilization of sample preparation equipment. sampling spoon, scissors and so on.
4.6 Sample dilution bottles, test tubes, straws, plates, ring vaccination, slides and so on.
4.7 Syringe. 1mL.
4.8 mice.
5 medium and reagents
5.1 NS (see Appendix A A.1 chapter).
5.2 cooked meat medium (see Appendix A, Section A.2 chapter).
5.3 blood agar plates (see Appendix A A.3 chapter).
5.4 Gram staining solution.
5.5 catalase test reagent.
5.6 tetanus antitoxin.
6 Clostridium tetani detection method
6.1 tests
Clostridium tetani testing procedures shown in Figure 1.
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