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SN/T 2206.12-2014 English PDF

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SN/T 2206.12-2014: Determination of microorganism in cosmetics - Part12: PCR method for Pseudomonas aeruginosa
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SN/T 2206.12-2014English319 Add to Cart 3 days [Need to translate] Determination of microorganism in cosmetics - Part12: PCR method for Pseudomonas aeruginosa Valid SN/T 2206.12-2014

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Standard similar to SN/T 2206.12-2014

QB/T 1975   Q/CT 2360   SN/T 2107   SN/T 2206.14   SN/T 2206.1   SN/T 2206.10   

Basic data

Standard ID SN/T 2206.12-2014 (SN/T2206.12-2014)
Description (Translated English) Determination of microorganism in cosmetics - Part12: PCR method for Pseudomonas aeruginosa
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 12,19
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB 7918.1; GB 7918.4; SN/T 1193
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the method of PCR detection of Pseudomonas aeruginosa in cosmetics. This standard applies to cosmetics for detecting Pseudomonas aeruginosa.

SN/T 2206.12-2014: Determination of microorganism in cosmetics - Part12: PCR method for Pseudomonas aeruginosa


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(Cosmetic microbiological test methods - Part 12. Pseudomonas aeruginosa PCR method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Cosmetic microbiological test method Part 12. Pseudomonas aeruginosa PCR. Part 12. PCRmethodforPseudomonasaeruginosa Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

SN/T 2206 "cosmetic microbiological test method" is divided into 13 parts. --- Part 1. Salmonella; --- Part 2. aerobic Bacillus subtilis and Bacillus cereus; --- Part 3. Klebsiella pneumoniae; --- Part 4. Streptococcus; --- Part 5. Enterococcus; --- Part 6. Clostridium tetani; --- Part 7. Western blot detection of BSE pathogen; --- Part 8. Candida albicans; --- Part 9. bile acid tolerance Gram-negative bacteria; --- Part 10. Staphylococcus aureus PCR method; --- Part 11. Staphylococcus aureus multiplex real-time PCR method; --- Part 12. Pseudomonas aeruginosa PCR method; --- Part 13. maltophilia Aeromonas. This section SN/T 2206 Part 12. This section drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This section proposed and managed by the National Certification and Accreditation Administration Committee. This section was drafted by. People's Republic of China Shandong CIQ. The main drafters of this section. Sun Jun, Zhao Han, Tianguo Ning, Zhang Jinling, Gong Xiao Ming, Hao Ying, Wang Hongbing Zhang Yibing, Sun Tao. Cosmetic microbiological test method Part 12. Pseudomonas aeruginosa PCR.

1 Scope

This section SN/T 2206 provides for PCR detection of Pseudomonas aeruginosa in cosmetics. This section applies to cosmetics for detecting Pseudomonas aeruginosa.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB 7918.1 General Cosmetics Standard methods of microbiological GB 7918.4 Cosmetics Standard methods of microbiological Pseudomonas aeruginosa SN/T 1193 genetic testing laboratory technical requirements 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this document. 3.1 Terms and Definitions 3.1.1 Pseudomonas aeruginosa Pseudomonasaeruginosa Also called Pseudomonas aeruginosa, a capsule, flagella, Gram-negative bacteria spore-free, can break down proteins, carbohydrates fermentation capacity than Low, can produce pyocyanin and fluorescein. 3.2 Acronyms PCR. polymerase chain reaction, referred to as PCR (polymerasechainreaction) DNA. deoxyribonucleic acid (deoxyribonucleicacid) dNTP. deoxynucleotide triphosphate (deoxyribonucleosidetriphosphate) Taq enzyme. TaqDNA polymerase

4 Method summary

Cosmetics After enrichment, genomic DNA, to extract the DNA as a template Pseudomonas exotoxin A gene highly conserved pieces PCR amplification segment, agarose gel electrophoresis test whether the 397bp PCR product characteristic bands, thereby cosmetics for contamination Pseudomonas aeruginosa quick test.

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