|
US$209.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
LY/T 3186-2020: Technological regulation for seedling breeding of wild plant with extremely small populations
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| LY/T 3186-2020 | English | 209 |
Add to Cart
|
3 days [Need to translate]
|
Technological regulation for seedling breeding of wild plant with extremely small populations
| Valid |
LY/T 3186-2020
|
PDF similar to LY/T 3186-2020
Basic data | Standard ID | LY/T 3186-2020 (LY/T3186-2020) | | Description (Translated English) | Technological regulation for seedling breeding of wild plant with extremely small populations | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B60 | | Classification of International Standard | 65.020 | | Word Count Estimation | 9,994 | | Date of Issue | 2020-03-30 | | Date of Implementation | 2020-10-01 | | Regulation (derived from) | Announcement No. 6 of 2020 by the State Forestry and Grassland Bureau | | Issuing agency(ies) | State Forestry and Grassland Administration | LY/T 3186-2020: Technological regulation for seedling breeding of wild plant with extremely small populations
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technological regulation for seedling breeding of wild plant with extremely small populations
ICS 65.020
B60
LY
People's Republic of China Forestry Industry Standard
Technical Regulations for Seedling Breeding of Very Small Population of Wild Plants
2020-03-30 released
2020-10-01 implementation
Issued by the State Forestry and Grassland Administration
Table of contents
Foreword...II
1 Scope...1
2 Normative references...1
3 Terms and definitions...1
4 Breeding principles...2
5 Sexual reproduction...2
6 Asexual reproduction...5
7 Nursery file management...7
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the State Forestry and Grassland Administration.
The organization responsible for drafting this standard. Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Wuhan University, Chinese Academy of Sciences
South Botanical Garden.
The main drafters of this standard. Zang Runguo, Li Jiaru, Huang Jihong, Wu Kunlin, Ding Yi, Xu Yue, Lu Xinghui.
Technical Regulations for Seedling Breeding of Very Small Population of Wild Plants
1 Scope
This standard regulates the principles, sexual and asexual breeding techniques for seedling breeding of very small populations of wild plants.
This standard applies to all kinds of wild plants with very small populations in my country. The protection of other rare and endangered plants can be referred to.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6001 Technical Regulations for Nursery
GB/T 16619 Forest Tree Seed Collection Technology
LY/T 1882--2010 Technical Regulations for Tissue Culture and Seedling of Forest Trees
LY/T 1000--2013 Container Seedling Technology
LY/T 2652-2016 Technical specifications for the protection and propagation of very small populations of wild plants
3 Terms and definitions
GB/T 6001, GB/T 16619, LY/T 1882-2010, LY/T 1000-2013 and LY/T 2652-2016, as well as the following
The terms and definitions apply to this standard.
3.1 Wild plant with extremely small populations
It refers to the narrow distribution area, long-term interference from external factors, showing population degradation and continuous decrease in the number of individuals, population and individual
The population is extremely small, the smallest living population that has fallen below the limit of stable survival, and the wild plants that are on the verge of extinction at any time. Mainly include the following 3 types
Type. (1) The wild population is extremely small, extremely endangered, and is at risk of extinction at any time; (2) Unique habitat requirements and narrow ecological range; (3) Potential base
Because the value is not clear, its extinction will cause gene loss, reduction of biodiversity, huge loss of socio-economic value and relatively small population.
3.2 sexual breeding
Also known as seed breeding, it means that plants use the seeds formed by double fertilization of male and female reproductive cells produced by parents to reproduce offspring.
process.
3.3 asexual breeding
The process of plant reproduction does not involve the fertilization process of reproductive cells, and the process of breeding new individuals directly from a part of the mother’s body, including skewer
Various forms such as insertion, grafting and tissue culture.
3.4 explant
Organs or tissue fragments used as materials for in vitro culture in plant tissue culture.
3.5 Primary culture
Refers to the process of cultivating cells, tissues and organs directly from the body.
3.6 Subculture
It means that after the callus grows on the medium for a period of time, the nutrients are exhausted, the water is lost, and some metabolites have been accumulated.
It is necessary to transfer these tissues to a new medium for cultivation.
3.7 grafting propagation
It is a mode of asexual reproduction in which a part of plant vegetative organs is transplanted to other plants.
3.8 Cuttage propagation
Take a part of the plant's vegetative organs and insert it into the loose and moist soil or fine sand, and use its regeneration ability to make it root and branch.
Asexual reproduction of new plants. According to the different organs used, there are branch cuttings, leaf cuttings, root cuttings and bud cuttings.
4 Principles of breeding
4.1 The collection of materials should avoid affecting the growth, reproduction and regeneration of the original mother plant.
4.2 Maximize the number of individuals in the population through various methods of reproduction.
4.3 The number of breeding individuals of different phenotypes and different genotypes remains balanced.
5 Sexual reproduction
5.1 Seed collection
5.1.1 Preparation
5.1.1.1 Before seed collection, determine the location and location of the species to be collected based on the individual population situation and seed demand combined with actual field surveys.
The number and period of seed collection.
5.1.1.2 Develop a seed collection plan, clarify seed collection methods and division of responsibilities, and prepare seed collection facilities and tools.
5.1.1.3 Observe the seed maturation process in time, grasp the characteristics of seed maturity, clarify the time of seed shedding, determine and announce the seed collection period.
5.1.1.4 Organize professional training for seed collectors.
5.1.2 Acquisition
5.1.2.1 Choose the mature plant that grows vigorously and is free of diseases and insect pests as the seed collection mother plant.
5.1.2.2 Strictly follow the safety protection measures to ensure the personal safety of the collectors.
5.1.2.3 The collection, labeling, packaging, transportation and storage must be carried out by trained professionals.
5.1.2.4 The collected seeds need to be temporarily encapsulated in a breathable cloth bag, attached with a label, and transported to the storage location as soon as possible.
5.1.2.5 The person in charge of seed collection carefully fills in the seed collection label and fills in the seed collection registration form.
5.1.2.6 The seed collection registration form must include important information such as the collector, collection time, location, and habitat conditions. The seed collection registration form shall refer to GB/T
16619 Appendix E. Fill in the contents of the registration form for forest tree collection.
5.1.3 Number of collections
5.1.3.1 At least 5 populations are collected for each species, and if there are less than 5 populations, each population must be collected.
5.1.3.2 At least 50 individuals shall be collected from each population. If there are less than 50 individuals, each individual must be collected.
5.1.3.3 The amount of seeds collected by each individual shall be judged with reference to the estimated number of individuals in the population and the amount of individual seeds.
a. When the number of individuals in the population is greater than or equal to 100, each body shall be collected at 1/10 of the estimated seed setting;
b. When the number of individuals in the population is between 10-100, each body shall be collected at 1/5 of the estimated seed setting;
c. When the number of individuals in the population is less than or equal to 10, each body collects as many seeds as possible;
d. When the number of population individuals is greater than or equal to 100, collect seeds from different individuals every year.
5.2 Seed preservation
According to the physiological and ecological characteristics of different plant seeds, physical and chemical methods are used for preservation.
5.3 Seed nursery
5.3.1 Methods of raising seedlings
Use containers to grow seedlings.
5.3.2 Types of nursery containers
5.3.2.1 Plastic film container
It is made of a non-toxic plastic film with a thickness of 0.02 mm-0.06 mm, a diameter of 5 cm and a height of 12 cm. The bottom needs to be punched with 2-6 diameters
Small holes of 0.4 cm-0.6 cm.
5.3.2.2 Plastic Flat Plate
Made of polyethylene foam or non-toxic plastic, the length, width and height are 54 cm, 28 cm and 5 cm respectively, and the bottom of the plate is every 0.5 cm
Holes with 0.5 cm intervals are arranged regularly.
5.3.2.3 Hard plastic cup
A cylindrical container is made of hard plastic, with drainage holes at the bottom. There are 3-4 prismatic protrusions on the inner wall of the container.
5.3.2.4 Plastic plug tray
Made of polyethylene foam or non-toxic plastic, the length and width are 54 cm and 28 cm respectively, the height is generally 5-12 cm, and the holes are large
The size is generally 3-4 cm.
5.3.3 Seedling substrate
5.3.3.1 Matrix composition
a. The seedling substrate should be made of local materials, with good physical and chemical properties such as moisture retention, ventilation and water permeability, and light weight, free of germs, eggs, seeds
Sundries such as seeds and stones;
b. The materials for the preparation of the substrate include yellow heart soil, fired soil, humus soil, peat, vermiculite, perlite, decomposed crop stalks, rice
Husk, bark powder and sawdust, etc., are prepared according to the cultivated species and mixed in a certain proportion;
c. Refer to Appendix B of LY/T 1000-2013 for commonly used matrix components and their ratios;
d. The substrate can be added with an appropriate amount of base fertilizer. the amount is determined according to the species, cultivation period, seedling specifications, container size and substrate fertility,
Use more organic fertilizers for broad-leaved trees, and increase phosphorus and potassium fertilizers for coniferous trees.
5.3.3.2 Sterilization and pH adjustment of the substrate
a. The substrate should be strictly disinfected, please refer to Appendix C of LY/T 1000-2013;
b. When preparing the substrate, the pH must be adjusted to the appropriate range for the seedling species.
5.3.3.3 Mycorrhizal inoculation
After the substrate is disinfected, the species that needs to be inoculated with mycorrhizal should be inoculated with mycorrhizal soil or strains.
5.3.4 Container seedling cultivation
5.3.4.1 Preparation before nursery
a. Nursery field conditions. a test greenhouse with complete ventilation, temperature and moisture control;
b. Filling the substrate. Sprinkle the well-mixed and homogeneous substrate, moisten it with water, and fill the container;
c. Container placement. Place the container with the substrate neatly on a flat field or on a container rack.
5.3.4.2 Seed disinfection and germination
Before sowing, the seeds should be disinfected and germinated. For specific methods, refer to Appendix B and Appendix C in GB/T 6001.
5.3.4.3 Sowing period and sowing amount
a. The sowing period should be determined according to the characteristics of the species, environmental conditions, seedling period, transplanting time and other factors;
b. The sowing amount should be determined according to species characteristics, germplasm quality and quantity.
5.3.4.4 Cover soil
Whether to cover the soil or the thickness of the cover varies from species to species. The unpleasant seeds need to be covered with soil, and the thickness is 1-3 times the seed thickness. After covering the soil, pour immediately
Water, the seedling stage should keep the substrate moist.
5.3.4.5 Sprout transplantation
a. After the sprouts are unearthed, they are transplanted into containers, and the transplanting time is determined according to the characteristics of the species.
b. Before transplanting, water the seedbed thoroughly, take the sprouts and transplant them in the center of the container, the transplanting depth should be 0.5-1.0 cm above the root neck, 1 per container
Strain. After transplanting, water thoroughly and keep moist. Seedlings should be properly shaded.
5.3.5 Seedling management
5.3.5.1 Watering
Watering should be timely and appropriate, and should be watered frequently during the emergence and early stages of seedling growth to keep the substrate moist; the fast-growing period should be less frequent;
Watering should be controlled in the late growth period.
5.3.5.2 Top dressing
The time, frequency, type of fertilizer, and amount of topdressing should be determined according to the species and substrate fertility. According to the requirements of each developmental stage of the seedlings,
Adjust the ratio and application rate of nitrogen, phosphorus, and potassium. Nitrogen fertilizer is the mainstay in the fast-growing period. Nitrogen fertilizer is appropriately reduced or not applied in the late growth period.
Potash fertilizer.
5.3.5.3 Disease and pest control
a. According to the occurrence rules of various species of pests and diseases, prevent pests and diseases;
b. Focus on prevention and strengthen management measures;
c. Try to adopt artificial traps for pests that can be trapped;
b. Minimize the use of drugs for prevention and treatment, if necessary, strictly follow the dosage and type, and refer to GB/T 6001 Appendix E for the dosage.
5.3.5.4 Other management
a. Weeding. manual extraction is the main task, and the roots should be removed when the substrate is wet;
b. Substrate replenishment. The substratum in the container should be filled up in time to prevent the root system from being exposed and the accumulation of water to cause disease;
c. In the event of a temporary power failure in the greenhouse, it must be able to respond in time.
6 Asexual reproduction
6.1 Tissue culture
6.1.1 Selection and treatment of explants
6.1.1.1 According to the characteristics of the species, select the immature embryos, seeds and other sexual organs or sexual organs or seeds that grow on healthy plants in different growing seasons.
Roots, stems, leaves and other vegetative organs are used as explants.
6.1.1.2 The explants are disinfected before inoculation. The disinfection method varies with different species and different materials.
a. After collecting the explants, rinse the surface with running water for 15-20 minutes, and then wipe the surface with 75% alcohol.
b. Under aseptic conditions, use 10% sodium hypochlorite or 0.1% mercury liters for further disinfection for 5-20 minutes, and then rinse with sterile water for 3-5
Inoculate directly after the second time or absorb the water with sterile filter paper for later use.
6.1.2 Primary culture
a. Choose a suitable primary induction medium, according to different species, organ reproduction, callus and somatic cell regeneration can be used
In this way, cluster buds, callus or somatic embryos are induced.
b. The temperature of the culture room should be within the range of 22-30℃, and the light intensity should be 30-50 μmol·m-2·s-1.
c. For materials that are easy to brown, you can shorten the subculture cycle or add antioxidants such as vitamin C and sodium citrate to the medium
And activated carbon and other methods to reduce or avoid browning.
6.1.3 Proliferation culture
a. Choose a suitable proliferation medium and inoculate it in a clean bench.
b. Different materials and different propagation methods require suitable inoculation density.
c. The proliferation of cluster buds is usually 2-3 small buds as a unit.
d. The callus is usually the size of mung bean. Somatic embryos can be single or 3-5 clusters depending on the material.
e. The culture period is usually 25-60 d.
6.1.4 Differentiation culture
a. Differentiation culture is required when using callus and somatic cell regeneration.
b. Choose a suitable differentiation medium, and the culture period is 25-60 d.
6.1.5 Rooting culture
Choose a suitable rooting medium, and often inoculate the regenerated single shoots into culture flasks. The culture period is 21-60 d, and the height is 3-5 cm.
The whole plant.
6.1.6 Bottle seedling refinement
a. Tissue cultured seedlings improve the survival rate of transplanting through seedling refinement.
b. The environment for the cultivation of seedlings is generally a greenhouse for transplanting from the bottle, and the light intensity is 800-1000 μmol·m-2·s-1.
c. The cultivation time is about 1 week.
6.1.7 Transplanting bottle seedlings
a. After washing the root medium of the bottle seedlings with water, sterilize the bottle seedlings and place them on the shade net to drain the water until the roots are white.
plant.
b. Select substrates with less pathogenic bacteria, good air permeability and strong water retention capacity, such as sphagnum, peat soil, perlite and river mud in different proportions
The system of the matrix.
c. The substrate should be disinfected before transplanting.
d. Do a good job of preventing and controlling pests and diseases after transplanting.
6.2 Grafting
6.2.1 Scion collection and storage
a. Collect 1-2 year-old branches from mature mother plants or young plants.
b. Collect the sap before flowing, and store the branches in a cold storage (0-2°C) after collection.
6.2.2 Rootstock selection
Choose seedlings with strong growth and strong resistance.
6.2.3 Scion wax seal
Use a water bath to control the temperature of the wax to 80-90℃; use the quick dip wax method at both ends to seal the scion with wax as a whole; the wax layer is transparent and colorless.
6.2.4 Grafting method
Adopt bud grafting, graft grafting, high position grafting, flat grafting and other methods, according to the biological characteristics of different species, adopt different technical measures.
6.3 Cuttings
6.3.1 Seedbed preparation
a. The sandy loam with deep, loose, fertile, well-drained, neutral or slightly acidic soil should be selected. If the soil is not suitable, it should be changed first.
Good soil.
b. Use bricks to form a cutting bed with a width of about 90-120 cm and a height of 35-40 cm, with the guest soil as the seedbed substrate, and a 5 cm thick small bed at the bottom of the bed.
The gravel is then filled with 15-20 cm thick guest soil, and 15 cm thick river sand is added to the upper layer to facilitate drainage and ventilation.
6.3.2 Environmental control
a. Use plastic film small arch shed (50 cm high) to cover the entire seedbed to maintain the air humidity of the slotter and the water content of the mechanism (air humidity
80-90%, the humidity of the substrate is to the degree that the river sand is loosened and not agglomerated by hand pinching).
b. Pay attention to prevent excessive sunlight and high temperature, ventilate in time, and add shading nets (2 m high) on the arch, according to the light intensity
Adjust the transmittance of the sunshade net in time with the change of air temperature.
6.3.3 Insert selection
a. Choose healthy and vigorously growing plants as the mother plant.
b. Select two-year-old, high lignified hard branches or one-year-old semi-ligninized, disease-free, and full bud-eye tender branches as cuttings.
6.3.4 Insert collection
a. In the morning and evening on a sunny day or in the daytime on a cloudy, light rainy day, the cuttings must be collected to maintain the proper moisture of the branches and the wetness of the leaves
After tying, immerse the base in clean water to prevent the branches from drying out.
b. The collected cuttings are bundled by seed source, and the label is attached to indicate the basic information such as the collection location and the collector.
6.3.5 Cutting treatment
a. Cut the cuttings with buds at the semi-lignified parts of the cuttings.
b. The top is flat and the bottom is oblique, keeping 1/2 of the 1-3 leaves at the top of the cutting.
c. Soak the base of the cuttings with rooting agent.
d. The rooting agent should be prepared for immediate use.
6.3.6 Cuttings
The cutting depth is 1/2-2/3 of the length of the cuttings. After the cuttings are completed, pour water in time, cover the small arches of plastic film, and shade nets to avoid
Excessive sun exposure.
6.3.7 Post management
After the cuttings are completed, appropriate light, temperature, and moisture management should be adopted, and pests and diseases should be treated to prevent them from occurring.
7 Nursery file management
The nursery should establish files for basic information, technical management and scientific experiments. Refer to the attachment of GB/T 6001 for the technical management files of container seedlings
Record D.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of LY/T 3186-2020_English be delivered?Answer: Upon your order, we will start to translate LY/T 3186-2020_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of LY/T 3186-2020_English with my colleagues?Answer: Yes. The purchased PDF of LY/T 3186-2020_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|