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GB/T 30087-2013: Determination of phenylbutazone in cosmetics by high performance liquid chromatography method
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 30087-2013 | English | 229 |
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Determination of phenylbutazone in cosmetics by high performance liquid chromatography method
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GB/T 30087-2013
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Basic data | Standard ID | GB/T 30087-2013 (GB/T30087-2013) | | Description (Translated English) | Determination of phenylbutazone in cosmetics by high performance liquid chromatography method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 10,177 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Standards Bulletin No. 25 of 2013 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | The standard provides high-performance liquid chromatographic method Cosmetics mediator phenylbutazone content. This standard applies to the determination of agent class, cream lotion, lipstick and other cosmetics Mediator class phenylbutazone. | GB/T 30087-2013: Determination of phenylbutazone in cosmetics by high performance liquid chromatography method
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Determination of pheylbutazone in cosmetics by high performance liquid chromatography method
ICS 71.100.70
Y42
National Standards of People's Republic of China
Cosmetics Content Determination mediator Bute
High Performance Liquid Chromatography
Issued on. 2013-12-17
2014-03-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This standard was drafted in accordance with the rules of GB/T 1.1-2009 and GB/T 20001.4-2001 given.
This standard by the National Quality Supervision key product testing method Standardization Technical Committee (SAC/TC374) centralized.
This standard was drafted. Country cosmetics Quality Supervision and Inspection Center (Beijing), Beijing Haidian District, product quality supervision, inspection, inspection
Alliance (Beijing) Technology Research Institute of Quality.
The main drafters of this standard. Wang Hao, Liu Yanqin, Shihai Liang, Yang Hongmei, with Mu Na, Tian Yanling.
Cosmetics Content Determination mediator Bute
High Performance Liquid Chromatography
1 Scope
This standard specifies a high performance liquid chromatographic method cosmetic mediator phenylbutazone content.
This standard applies to the determination of agent class, cream lotion, lipstick and other cosmetics mediator Bute.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
Principle 3
Ultrasound sample methanol solution containing 2% by ammonia extraction and enrichment of the extract, measured by HPLC, external standard, liquid
GC/MS.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.1 ammonia.
4.2 ammonium acetate.
4.3 sodium chloride.
4.4 tetrahydrofuran. chromatography.
4.5 Methanol. chromatographically pure.
4.6 acetonitrile. chromatography.
4.7 2% methanol solution of ammonia. Pipette 10mL ammonia (4.1) to 500mL volumetric flask, methanol (4.5) volume.
4.8 Acetonitrile Methanol (1 2, V/V). Measure 250mL acetonitrile (4.6) was mixed with 500mL of methanol (4.5).
4.9 20mmoL/L ammonium acetate solution. Weigh 1.54g ammonium acetate (4.2) in a beaker, dissolved in water and dilute to 1000mL.
4.10 Phenylbutazone Standards. details are shown in Table 1.
Table 1 Phenylbutazone standard sample information
Chinese name English name CAS Registry Number Formula Molecular Weight Purity
Phenylbutazone Phenylbutazone 50-33-9 C19H20N2O2 308.38 ≥99%
4.11 standard stock solution. Weigh accurately 0.1g standard sample (4.10), accurate to 0.0001g, in 50mL beaker, it adds appropriate amount of methanol
(4.5) dissolved transferred to 100mL brown volumetric flask with methanol (4.5) volume. The solution was stored at -18 ℃ below, can save six
Months.
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