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GB/T 25223-2024: Animal and vegetable fats and oils - Determination of composition of sterols and total sterols contents - Gas chromatographic method
Status: Valid GB/T 25223: Evolution and historical versions
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| GB/T 25223-2024 | English | 519 |
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Animal and vegetable fats and oils - Determination of composition of sterols and total sterols contents - Gas chromatographic method
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GB/T 25223-2024
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| GB/T 25223-2010 | English | 314 |
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Animal and vegetable fats and oils -- Determination of individual and total sterols contents -- Gas chromatographic method
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GB/T 25223-2010
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PDF similar to GB/T 25223-2024
Basic data | Standard ID | GB/T 25223-2024 (GB/T25223-2024) | | Description (Translated English) | Animal and vegetable fats and oils - Determination of composition of sterols and total sterols contents - Gas chromatographic method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X14 | | Classification of International Standard | 67.200.10 | | Word Count Estimation | 26,244 | | Date of Issue | 2024-09-29 | | Date of Implementation | 2025-04-01 | | Older Standard (superseded by this standard) | GB/T 25223-2010 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 25223-2024: Animal and vegetable fats and oils - Determination of composition of sterols and total sterols contents - Gas chromatographic method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National Standard of the People's Republic of China
ICS 67.200.10CCS X 14
Sterol composition and sterols of animal and plant fats
Determination of total amount by gas chromatography
Animal and vegetable fats and oils-Determination of composition of
sterols and total sterols contents-Gas chromatographic method
(ISO 12228⁃1.2014, Determination of individual and total sterols contents-
Gas chromatographic method-Part 1.Animal and vegetable fats and oils,
ISO 12228⁃2.2014, Determination of individual and total sterols contents-
Gas chromatographic method-Part 2.Olive oils and olive pomace oils, MOD)
2024-09-29 Release
2025-04-01 Implementation
State Administration for Market Regulation
The National Standardization Administration issued
Replace GB/T 25223-2010
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1.Structure and drafting rules for standardization documents"
Drafting is required.
This document replaces GB/T 25223-2010 "Determination of sterol composition and total sterol content in animal and vegetable oils and fats - Gas chromatography method"
Compared with GB/T 25223-2010, in addition to structural adjustments and editorial changes, the main technical changes are as follows.
-- Added Method 2.Determination of sterol composition and total sterol content in olive oil and olive-pomace oil (see Chapter 5).
This document is modified to adopt ISO 12228-1.2014 "Determination of sterol composition and total sterol content by gas chromatography - Part 1.Animal
Vegetable oils and fats” and ISO 12228-2.2014 “Determination of sterol composition and total sterol content by gas chromatography Part 2.Olive oil and olive oil
Olive pomace oil.
Compared with ISO 12228-1.2014 and ISO 12228-2.2014, this document has many structural adjustments.
See Appendix A for a list.
The technical differences between this document and ISO 12228-1.2014 and ISO 12228-2.2014 and their reasons are as follows.
--ISO 3696 was replaced by the normative reference GB/T 6682 to adapt to my country's national conditions;
--ISO 661 was replaced by the normative reference GB/T 15687 to adapt to my country's national conditions.
The following editorial changes were made to this document.
--The name of the standard is changed to "Determination of sterol composition and total sterol content of animal and vegetable oils and fats - Gas chromatography";
-- Deleted the informative appendices that are not applicable in the international standards;
-- Deleted inapplicable references to international standards.
Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.
This document was proposed by the State Administration of Grain and Material Reserves.
This document is under the jurisdiction of the National Technical Committee on Standardization of Cereals and Oils (SAC/TC 270).
This document was drafted by. National Grain and Material Reserves Administration Scientific Research Institute, Jiangnan University, Shaanxi Keyi Sunshine Testing Technology Service Co., Ltd.
Company, Shandong Jinsheng Cereals and Oils Food Co., Ltd., Qingdao Tianxiang Xiyan Food Co., Ltd., Shandong Xiangchi Cereals and Oils Co., Ltd., Nanjing Cai
University of Finance and Economics, Feixian COFCO Oils & Fats Industry Co., Ltd., Changshouhua Food Co., Ltd., Shandong Longda Grain & Oil Co., Ltd., Rizhao Quality
Inspection and Testing Institute, Pony Testing Group Shanghai Co., Ltd.
The main drafters of this document are. Duan Zhangqun, Xue Yalin, Wang Xingguo, Chai Chengliang, Tu Jindi, Gao Guanyong, Yu Qiang, Ma Longkun, Yuan Jian, Liu Peilian,
Wang Mingxing, Yang Qian, Li Xiujuan, Liu Ruijie, Gao Hongliang, Zhang Nianying, Zhang Yingjie, Hou Guofeng, Xing Changrui, and Li Peng.
The previous versions of this document and the documents it replaces are as follows.
--First published in 2020 as GB/T 25223-2010;
--This is the first revision.
Sterol composition and sterols of animal and plant fats
Determination of total amount by gas chromatography
1 Scope
This document defines the terms and definitions of sterol composition and total sterol content in animal and vegetable fats and oils and describes the gas chromatographic method for the determination of sterol content in animal and vegetable fats and oils.
The principles, reagents, instruments, operating procedures, result expression and precision of sterol composition and total sterol content in vegetable oils and fats.
This document is applicable to the determination of sterol composition and total sterol content in animal and vegetable oils and fats. Among them, the second method is only applicable to olive oil and olive oil
Determination of sterol composition and total sterol content in pomace oil.
2 Normative references
The contents of the following documents constitute the essential clauses of this document through normative references in this document.
For referenced documents without a date, only the version corresponding to that date applies to this document; for referenced documents without a date, the latest version (including all amendments) applies to
This document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories (GB/T 6682-2008, ISO 3696.1987, MOD)
GB/T 15687 Preparation of animal and vegetable fat samples (GB/T 15687-2008, ISO 661.2003, IDT)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
composition of sterols
Individual sterol components from cholesterol to Δ7-avenosterol in the total amount of sterols measured under the conditions specified in this document.
Note. The component content is calculated by peak area normalization method and expressed as mass fraction.
3.2
Total sterol content
Under the conditions specified in this document, the total mass of all individual sterols measured from cholesterol to Δ7-avenosterol accounts for the mass of the sample.
Fraction.
Note. The content is expressed in milligrams per kilogram (mg/kg).
4 Method 1 Determination of sterol composition and total sterol content in animal and vegetable fats and oils (other than olive oil and olive pomace fats and oils)
4.1 Principle
The sample was saponified by reflux with potassium hydroxide-ethanol solution, and the unsaponifiable matter was separated by solid phase extraction using an alumina column.
The ions are adsorbed by the alumina column and the sterols flow out of the column. The sterols are separated from the unsaponifiable matter by thin layer chromatography.
Betulin was used as an internal standard, and the sterol components and their contents were qualitatively and quantitatively analyzed by gas chromatography.
4.2 Reagents
Unless otherwise stated, only analytical grade reagents were used.
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