|
US$209.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 25221-2010: Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 25221-2010 | English | 209 |
Add to Cart
|
3 days [Need to translate]
|
Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography
| Valid |
GB/T 25221-2010
|
PDF similar to GB/T 25221-2010
Basic data | Standard ID | GB/T 25221-2010 (GB/T25221-2010) | | Description (Translated English) | Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X10 | | Classification of International Standard | 67.040 | | Word Count Estimation | 9,959 | | Date of Issue | 2010-09-26 | | Date of Implementation | 2011-03-01 | | Quoted Standard | GB 5491; GB/T 6682 | | Regulation (derived from) | National Standard Approval Announcement 2010 No.6 (Total No.161) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the terms and definitions of food in the determination of ergosterol normal phase high performance liquid chromatography, principle, reagents and materials, instruments and equipment, for sampling, sample preparation, steps, result of the calculation and representation, repeatability requirements. This standard applies to rice, determination of ergosterol in grain corn, wheat. The method detection resistor is 0. 05 mg/kg. | GB/T 25221-2010: Inspection of grain and oils -- Determination of ergosterol in grain -- Normal phase high performance liquid chromatography
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection of grain and oils.Determination of ergosterol in grain. Normal phase high performance liquid chromatography
ICS 67.040
X10
National Standards of People's Republic of China
Determination of ergosterol in foodstuffs by grain and oil
Normal Phase High Performance Liquid Chromatography
2010-09-26 release
2011-03-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People 's Republic of China
China National Standardization Management Committee released
Foreword
Appendix A to this standard is an informative appendix.
This standard is proposed by the National Grain Bureau.
This standard by the National Grain Standardization Technical Committee.
The standard responsible for the drafting unit. the State Grain Reserve Bureau of Chengdu Grain Storage Science Research Institute, Liaoning Province, grain and oil inspection and monitoring.
The main drafters of this standard. He Xuechao, Feng Yongjian, Jiang Tao, Zhong Yu, Xiao Xuebin, Wang Deqian, Xiong Shengwei, Qiao Lina.
Determination of ergosterol in foodstuffs by grain and oil
Normal Phase High Performance Liquid Chromatography
1 Scope
This standard specifies the terms and definitions of ergosterol in food by normal phase high performance liquid chromatography, principles, reagents and materials, instruments and
Equipment, sampling, sample preparation, operating procedures, calculation and presentation of results, repeatability requirements.
This standard applies to rice, wheat, corn and other food ergosterol determination.
The detection limit of this method is 0.05mg/kg.
2 normative reference documents
The terms of the following documents are hereby incorporated by reference into this standard. Whichever is the date of the reference file, which is followed by all
(Excluding corrigenda) or revisions do not apply to this standard, however, encourage the parties to reach an agreement under this standard
Whether you can use the latest version of these files. For undated references, the latest edition applies to this standard.
GB 5491 grain, oilseed inspection, sampling method
GB/T 6682 Analytical laboratory water specifications and test methods
3 terms and definitions
The following terms and definitions apply to this standard.
3.1
Ergosterol ergosterol
Derived from the infection of food fungi (spores or mycelia) produced free ergosterol and ergosterol phosphate material, is the fungal cells
Normal metabolites, and fungal cell membrane is one of the components.
4 principle
After saponification of the sample, extraction of ergosterol with n-hexane, the extract was dehydrated, concentrated and silica gel column separation, with high performance liquid chromatography violet
External detector at 282nm at the detection, external standard quantitative.
5 reagents and materials
Unless otherwise specified, all reagents are of analytical grade, and the test water shall comply with the requirements of Grade 3 water in GB/T 6682,
GB/T 6682 requirements for primary water requirements.
5.1 n-hexane. pure chromatography.
5.2 isopropyl alcohol. pure chromatography.
5.3 n-hexane. analytical grade for extraction.
5.4 Potassium hydroxide.
5.5 50% potassium hydroxide solution. Weigh 10 g of potassium hydroxide (5.4) and add 10 mL of water to dissolve.
5.6 Methanol.
5.7 2,6-di-tert-butyl-p-cresol (BHT).
5.8 Vitamin C (ascorbic acid).
5.9 Coke gallic acid.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB/T 25221-2010_English be delivered?Answer: Upon your order, we will start to translate GB/T 25221-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB/T 25221-2010_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 25221-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|