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Determination of lycopene, lutein and carotene in tomato products - Ultra performance liquid chromatography(UPLC) method
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GB/T 41133-2022
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Basic data Standard ID | GB/T 41133-2022 (GB/T41133-2022) | Description (Translated English) | Determination of lycopene, lutein and carotene in tomato products - Ultra performance liquid chromatography(UPLC) method | Sector / Industry | National Standard (Recommended) | Classification of Chinese Standard | X10 | Word Count Estimation | 9,992 | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration |
GB/T 41133-2022: Determination of lycopene, lutein and carotene in tomato products - Ultra performance liquid chromatography(UPLC) method ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 67.080
CCSX10
National Standards of People's Republic of China
Lycopene, lutein, lycopene in tomato products
Determination of carotene content
ultra-high performance liquid chromatography
Published on 2022-03-09
2022-10-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration
foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
drafted.
Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents.
This document is proposed and managed by the National Technical Committee on Standardization of Inspection Methods for Key Products of Quality Supervision (SAC/TC374).
This document is drafted by. Shanghai Institute of Quality Supervision and Inspection Technology, CCIC Warner (Beijing) Quality Technology Center Co., Ltd., Warner
General Standard (Beijing) Certification Co., Ltd.
The main drafters of this document. Weng Shiyu, Lin Lin, Lin Yikan, Huang Yuqing, Zhang Wen, Zheng Yi, Ge Yu, Chen Yufei, Meng Jie, Zheng Cunzhe.
Lycopene, lutein, lycopene in tomato products
Determination of carotene content
ultra-high performance liquid chromatography
1 Scope
This document specifies the principles, reagents and methods for the determination of lycopene, lutein and carotene in tomato products by ultra-high performance liquid chromatography.
Materials, equipment, analytical procedures, calculation of results, precision, limits of detection, and limits of quantification.
This document applies to lycopene, lutein, alpha-carotene and beta-carotene in tomato powder, tomato paste, tomato beverages, and dried tomato products
element determination.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations
documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to
this document.
GB/T 6682 Analysis Laboratory Water Specifications and Test Methods
3 Terms and Definitions
There are no terms and definitions that need to be defined in this document.
4 Principles
After adding water and ethanol to the sample, extract the lycopene, lutein and carotene in it with ether-n-hexane-cyclohexane mixed solution.
Separation by high performance liquid chromatography, detection by UV detector or diode array detector, and quantification by external standard method.
5 Reagents and Materials
Unless otherwise specified, use only chromatographically pure reagents.
5.1 Water. GB/T 6682, Class I.
5.2 Cyclohexane (C6H12).
5.3 Diethyl ether [(C2H5)2O].
5.4 n-hexane (C6H14).
5.5 Anhydrous ethanol (C2H5OH). analytically pure.
5.6 Dibutylhydroxytoluene (C15H24O, BHT). analytically pure.
5.7 Methanol (CH3OH).
5.8 Dichloromethane (CH2Cl2).
5.9 Extraction solvent. weigh 1.0 g of BHT (5.6) with an analytical balance (6.3), dissolve it in 400 mL of n-hexane (5.4), add.200 mL of cyclohexane
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