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GB/T 23378-2009 English PDF

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GB/T 23378-2009: Method for the determination of Neotame in foods -- High-performance liquid chromatography
Status: Obsolete
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GB/T 23378-2009English239 Add to Cart 3 days [Need to translate] Method for the determination of Neotame in foods -- High-performance liquid chromatography Obsolete GB/T 23378-2009

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Basic data

Standard ID GB/T 23378-2009 (GB/T23378-2009)
Description (Translated English) Method for the determination of Neotame in foods -- High-performance liquid chromatography
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard X40
Classification of International Standard 67.050
Word Count Estimation 6,688
Date of Issue 2009-04-08
Date of Implementation 2009-05-01
Quoted Standard GB/T 6682
Regulation (derived from) National Standard Approval Announcement 2009 No.3 (Total No.143)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China
Summary This standard specifies the method for determining the content of neotame in foods by high performance liquid chromatography. This standard applies to the determination of foods neotame.

GB/T 23378-2009: Method for the determination of Neotame in foods -- High-performance liquid chromatography


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Method for the determination of Neotame in foods. High-performance liquid chromatography ICS 67.050 X40 National Standards of People's Republic of China Determination in foods neotame High Performance Liquid Chromatography Posted 2009-04-08 2009-05-01 implementation Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China Standardization Administration of China released

Foreword

Appendix A of this standard is an informative annex. This standard by the national food safety emergency standardization working group proposed and managed. Drafting of this standard. National Food Quality Supervision and Inspection Center (Shanghai), Shanghai Institute of Technical Supervision quality inspection. The main drafters of this standard. Cao Chengming, Yin Jie, Li Jun, Yu Ge. Determination in foods neotame High Performance Liquid Chromatography

1 Scope

This standard specifies the method for determining the content of neotame in foods by high performance liquid chromatography. This standard applies to the determination of foods neotame.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD) Principle 3 The sample was buffer extraction, solid phase extraction (SPE) by HPLC, external standard.

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 acetonitrile. chromatography. 4.2 octane sulfonate. chromatography. 4.3 phosphoric acid. 4.4 formic acid. Chromatographic pure. 4.5 Methanol. chromatographically pure. 4.6 Neotame standards. purity ≥99.0%. 4.7 Mixed extracts. Pipette 0.8mL and 2.5mL of triethylamine formate, add water to 1000mL, pH value of about 4.5. 4.8 ion pair reagent buffer. Weigh 2.00g octane sulfonate, with the amount dissolved in water, phosphoric acid was added 1.0mL, add water to 1000mL. 4.9 C18 solid phase extraction column. 6mL, 500mg, SupelcleanTM LC-18, or rather who, prior to use successively with methanol 5mL, 10mL Water activated. 4.10 standard stock solution. Weigh accurately 0.1000g neotame standards, plus mixed extract dissolved volume to 100mL, the solution containing neotame An amount of 1.00mg/mL. 4.11 standard solution. lessons Neotame stock standard solution 1.0mL to 100mL volumetric flask, mix the extract to volume, this solution Neotame liquid content of 10.0μg/mL. 4.12 microporous membrane. 0.45μm, organic phase. 5. Apparatus 5.1 High Performance Liquid Chromatography (HPLC) instrument. with a UV detector or diode array detector. 5.2 ultrasonic cleaning. 5.3 vortex mixer.

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