GB 31645-2018 PDF English
US$85.00 · In stock · Download in 9 secondsGB 31645-2018: National food safety standard -- Collagen peptide Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 31645-2018 | English | 85 |
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National food safety standard -- Collagen peptide
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GB 31645-2018: National food safety standard -- Collagen peptide---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB31645-2018
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard - Collagen Peptide
Issued on. JUNE 21, 2018
Implemented on. DECEMBER 21, 2018
Issued by. National Health Commission of the People’s Republic of China;
State Administration for Market Regulation.
Table of Contents
1 Scope... 3
2 Terms and Definitions... 3
3 Technical Requirements... 3
Appendix A... 6
National Food Safety Standard - Collagen Peptide
1 Scope
This Standard is applicable to collagen peptide products for the purpose of food
processing.
2 Terms and Definitions
2.1 Collagen Peptide
Collagen peptide is a product that takes fresh animal tissues (including skin, bone,
muscle, tendon and scale, etc.) that contain sufficient collagen as the raw material. It
is produced through extraction, hydrolysis and refining; its molecular mass is lower
than 10,000.
3 Technical Requirements
3.1 Raw Material Requirements
3.1.1 Raw materials that can be adopted.
3.1.2 Raw materials that are prohibited.
3.2 Sensory Requirements
Sensory requirements shall comply with the stipulation in Table 1 below.
3.3 Physical and Chemical Indicators
Physical and chemical indicators shall comply with the stipulation in Table 2 below.
3.5 Microorganism Limit
Microorganism limit shall comply with the stipulation in Table 4 below.
Appendix A
Test Method of Proportion of Collagen Peptide with Relative Molecular Mass
Less Than 10,000
(High-performance size exclusion chromatography)
A.1 Method Summary
High-performance size exclusion chromatography shall be adopted in determination.
Specifically speaking, adopt porous filler as the stationary phase; separate in
accordance with the difference in molecular volume of the sample components; test
under the condition of 220 nm ultraviolet absorption wavelength of peptide bond. Adopt
exclusive data processing software (namely, GPC software) for the determination of
relative molecular mass distribution; process the chromatogram and the data of
standard substance and the sample. In accordance with relative molecular mass
correction curve equation, calculate the relative molecular mass and distribution range
of collagen peptide.
A.2 Reagents
A.2.1 Acetonitrile. chromatographic purity.
A.2.2 Trifluoroacetate. analytical purity.
A.2.3 Water. Grade-1 water stipulated in GB/T 6682.
A.2.4 Standard substances applied in relative molecular mass correction curve.
A.3 Instruments and Equipment
A.3.1 High-performance liquid chromatograph. equipped with UV detector and
chromatography workstation that contains GPC data processing software.
A.3.2 Mobile phase vacuum filtration degasser.
A.3.3 Ultrasonic oscillator.
A.3.4 Analytical balance. division value. 0.0001 g.
A.4 Chromatographic conditions and System Adaptability Experiment
Respectively use mobile phase to prepare peptide standard solutions of the above-
mentioned different relative molecular mass at the concentration of around 1.0 g/L.
Use polytetrafluoroethylene (PTFE) or nylon filter membrane (aperture. 0.2 μm ~ 0.5
μm) to filter it. Then, respectively inject it; obtain the chromatogram of series standard
substance. Use the logarithm of relative molecular mass (lgMW) to draw the retention
time or conduct linear regression. Thus, obtain the relative molecular mass correction
curve and its equation.
A.6 Sample Preparation
Use weighing paper to weigh-take around 125.0 mg of sample; transfer it into a 25 mL
volumetric flask. Use mobile phase to fixate it at a constant volume. Start ultrasonic
oscillation for 10 min, so that the sample can be thoroughly dissolved and mixed. Use
polytetrafluoroethylene (PTFE) or nylon filter membrane (aperture. 0.2 μm ~ 0.5 μm)
to filter it. Reserve the filtrate for determination.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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