|
US$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 25532-2010: National food safety standards of food additive natamycin
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB 25532-2010 | English | 189 |
Add to Cart
|
3 days [Need to translate]
|
National food safety standards of food additive natamycin
| Valid |
GB 25532-2010
|
PDF similar to GB 25532-2010
Basic data | Standard ID | GB 25532-2010 (GB25532-2010) | | Description (Translated English) | National food safety standards of food additive natamycin | | Sector / Industry | National Standard | | Classification of Chinese Standard | C54;X40 | | Classification of International Standard | 67.220.20 | | Word Count Estimation | 8,815 | | Date of Issue | 2010-12-21 | | Date of Implementation | 2011-02-21 | | Regulation (derived from) | Ministry of Health Bulletin No. 19 of 2010 | | Issuing agency(ies) | Ministry of Health of the People's Republic of China | | Summary | This Chinese standard applies to Natal Streptomyces (Streptomyces natalensis) obtained after controlled fermentation of a food additive natamycin. | GB 25532-2010: National food safety standards of food additive natamycin---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
National food safety standards of food additive natamycin
National Food Safety Standard
Food additives Natamycin
Issued on. 2010-12-21
2011-02-21 implementation
National Standards of People's Republic of China
People's Republic of China Ministry of Health issued
Foreword
Appendix A of this standard is a normative appendix.
National Food Safety Standard
Food additives Natamycin
1 Scope
This standard applies to the post-Natal Streptomyces (Streptomyces natalensis) controlled fermentation of food additives Natamycin.
2 Normative references
The standard file referenced in the application of this standard is essential. For cited documents with dates, only the date of
Version applies to this standard. For undated references, the latest edition (including any amendments) applies to this standard.
3 formula, relative molecular mass and structural formula
Formula 3.1
C33H47NO13
3.2 formula
3.3 relative molecular mass
665.73 (according to 2007 international relative atomic mass)
4. Technical Requirements
4.1 Sensory requirements. comply with Table 1.
Table 1 Sensory requirements
Project requires test methods
Color white to creamy yellow proper amount of sample is placed in a clean, dry white porcelain dish, natural
Light, observe its color and texture, and smell its flavor. Odour almost odorless
Organizational status crystalline powder
4.2 Physical indicators. to comply with Table 2.
Table 2. Physical and chemical indicators
Item Index Test Method
Natamycin (dry basis), w /% ≥ 95.0 Appendix A A.3
Specific rotation αm (20 ℃, D)/[(º) · dm2 · kg-1] 250 ~ 295 Appendix A A.4
Loss on drying, w /% ≤ 8.0 GB 5009.3 vacuum drying method
Residue on ignition, w /% ≤ 0.5 GB/T 9741a
pH 5.0 ~ 7.5 in Appendix A A.5
Lead (Pb)/(mg/kg) ≤ 2 GB 5009.12
He said a sample of about 2g.
Appendix A
(Normative)
Testing method
A.1 General Provisions
Unless otherwise indicated in the analysis using only recognized as analytical reagents and GB/T 6682-2008 specified in the water. In the analysis
Standard titration solution, impurities measured by standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601, GB/T
602, the provisions of preparation GB/T 603's. This solution was used in the test does not indicate what is formulated with solvent, it refers to an aqueous solution.
A.2 Identification Test
A.2.1 solubility
Insoluble in water, slightly soluble in methanol. Dissolved in glacial acetic acid and dimethylformamide.
A.2.2 color reaction
Take appropriate sample is placed in a dropping test board, dropping hydrochloric acid, to produce blue; dropping acid, to produce green, a few minutes later becomes reddish
color. Dropping amount of acid to be able to completely wetted or dissolved natamycin is satisfied that the sample for the degree.
A.2.3 UV absorption spectrum
Weigh accurately 25mg sample was transferred to 100mL volumetric flask, add 5.0mL water wet samples. Add 50mL glacial acetic acid - methanol solution
Liquid [acetic acid. methanol = 11000 (. volume ratio)], in the absence of light and shaken until dissolved. And then glacial acetic acid - methanol solution to volume,
Mix well. Pipette 2.0mL of the solution was placed in 100mL flask with glacial acetic acid - methanol solution to volume, and mix. The solution
Ultraviolet absorption spectrum as compared with the standard sample solution, having the same minimum and maximum absorption wavelength.
A.3 Determination of Natamycin
A.3.1 Reagents and materials
a) Natamycin standards.
b) acetonitrile. chromatography.
c) ammonium acetate.
d) ammonium chloride.
e) tetrahydrofuran.
f) methanol.
g) hydrochloric acid solution. 0.1mol/L.
A.3.2 Instruments and Equipment
High performance liquid chromatograph. with UV detector (detection wavelength 303nm).
A.3.3 reference chromatographic conditions
a) Column. 25cm × 4.6mm (diameter) column filled with octadecyl silica gel; or other equivalent column.
b) Mobile phase. Weigh 3.0g of ammonium acetate and 1.0g of ammonium chloride, dissolved in 760mL of water, and mix. Add 5.0mL of tetrahydrofuran and 240mL
Acetonitrile, mix and filter with 0.45μm membrane filter.
c) Column temperature. room temperature.
d) Mobile phase flow rate. 3mL/min.
e) Injection volume. 20 μL
NOTE. System Suitability injection is repeated three times standard solution, the resulting response to the relative deviation of the mean area of less than 1.0%.
A.3.4 Analysis step
A.3.4.1 Preparation of standard solutions
Weigh about 0.02g natamycin standard, accurate to 0.0001g, set 100mL flask, add 5.0mL of tetrahydrofuran,
Ultrasonic vibration 10min. 60mL of methanol was added, rotate until dissolved. Was added 25mL of water, cooled to room temperature after mixing. Add water to
The mark, mix and filter with 0.45μm membrane filter.
A.3.4.2 Preparation of sample solution
Weigh about 0.02g sample (accurate to 0.0001g), was transferred to 100mL volumetric flask 5.0mL tetrahydrofuran, ultrasonic shock
Swing 10min. 60mL of methanol was added, rotate until dissolved. Was added 25mL of water, cooled to room temperature after mixing. Add water to volume, mix
Uniform, filtered through a 0.45μm membrane filter.
A.3.4.3 Determination
In A.3.3 reference chromatographic conditions, respectively, the standard solution and the sample solution were measured, recorded peak area, calculated according to the formula
Sample natamycin content.
A.3.5 Calculation Results
Natamycin content X1 according to formula (A.1) Calculated.
X1 0
1 × ⎟⎟⎠
⎜⎜⎝
⎛ × ⎟⎟⎠
⎜⎜⎝
- ×
× =
SS
CMX) (% (A.1)
Where.
X1-- sample natamycin content,%;
MS-- natamycin standard mass in grams (g);
CS-- Natamycin standards in marked natamycin content,%;
MU-- sample mass, in grams (g);
X0-- sample measured loss on drying,%;
AU-- sample liquid chromatographic peak area obtained;
AS-- chromatographic analysis standard solution peak area obtained.
The results parallel arithmetic mean of the measurement results shall prevail. Twice in the same condition absolutely independent determination results
The difference is not more than 2% of the arithmetic mean.
A.4 Determination of specific rotation
A.4.1 weighed amount of sample in glacial acetic acid as solvent to prepare a sample solution of glacial acetic acid 10mg/mL, with the polarimeter is measured,
While doing the blank control. The measurement temperature was 20 ℃ ± 0.5 ℃.
Specific rotation αm (20 ℃, D) value to (°) · dm2 · kg-1 according to formula (A.2) Calculated.
(A.2)
Where.
α - measured angle of rotation, in degrees (°);
l - the length of the optical tube unit decimeter (dm);
()
αρ
αα
DCm = °, 20
ρα - mass concentration in the solution effective component in grams per milliliter (g/mL).
A.4.2 Other conducted in accordance with GB/T 613-2007 in.
A.5 pH measurement
Weighed amount of sample, the preparation of aqueous samples 10mg/mL, with the pH was measured by pH.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of GB 25532-2010_English be delivered?Answer: Upon your order, we will start to translate GB 25532-2010_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of GB 25532-2010_English with my colleagues?Answer: Yes. The purchased PDF of GB 25532-2010_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|