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GB 28307-2012 PDF English


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GB 28307-2012: PDF in English

GB 28307-2012 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard Food Additive - Maltitol and Maltitol Syrup (Including No.1 “Amendment Sheet”, September 22, 2015) ISSUED ON. APRIL 25, 2012 IMPLEMENTED ON. JUNE 25, 2012 Issued by. Ministry of Health of PRC Table of Contents 1 Scope ... 3  2 Chemical name, formula and relative molecular mass ... 3  3 Product classification ... 3  4 Technical requirements ... 4  Appendix A ... 5  National Food Safety Standard Food Additive - Maltitol and Maltitol Syrup 1 Scope This Standard applies to food additive - maltitol and maltitol syrup, which take starch as raw material, are processed and manufactured by liquefaction, saccharification, hydrogenation, refinement. 2 Chemical name, formula and relative molecular mass 2.1 Chemical name 4-O-α-D- glucopyranosyl sorbitol. 2.2 Formula C12H24O11. 2.3 Relative molecular mass 344.31 (in accordance with 2007 international relative atomic mass). 3 Product classification 3.1 Maltitol 3.1.1 Maltitol (category I) High purity crystalline product. 3.1.2 Maltitol (category II) Non-crystalline solid product directly dried by maltitol syrup. 3.2 Maltitol syrup Liquid mixture composed by a large number of maltitol, a small amount of sorbitol, oligosaccharide alcohol and polysaccharide alcohol. Appendix A Inspection methods A.1 General rules Reagents and water used in this Standard, in the absence of other requirements specified, all refer to analytical reagents and grade three water stipulated in GB/T 6682-2008. Standard solution used in test, standard solution, preparations and products used in determination of impurities, in the absence of other requirements specified, are prepared in accordance with provisions in GB/T 601, GB/T 602, GB/T 603. Solution in test all refers to aqueous solution when it does not indicate which solvent shall be used for preparation. A.2 Identification test A.2.1 Reagents and materials A.2.1.1 Water. GB/T 6682-2008, grade one water. A.2.1.2 Maltitol standard product. mass fraction ≥ 99.0%. A.2.2 Apparatus A.2.2.1 High performance liquid chromatography. equipped with refractive index detector, or other equivalent detectors. A.2.2.2 Mobile phase filtration vacuum degasser and 0.22 µm membrane. A.2.2.3 Chromatographic column. specially used for analysis of sugars or sugar alcohols which takes Ca type strong acid cation exchange resin as filler (column size. Φ 7.8 mm × 300 mm), or equivalent chromatographic column. A.2.3 Reference chromatographic conditions A.2.3.1 Mobile phase. GB/T 6682-2008, grade one water. A.2.3.2 Column temperature. 80°C. A.2.3.3 Mobile phase flow rate. about 0.5 mL/min. A.2.3.4 Injection volume. 20 µL. A.2.4 Analysis steps A.2.4.1 Preparation of standard solution A.2.3.3 Mobile phase flow rate. about 0.5 mL/min. A.2.3.4 Injection volume. 20 µL. NOTE. System suitability is to repeat injecting standard solution twice. Relative error of resulting response area is less than 20%. A.3.4 Analysis steps A.2.4.1 Preparation of sample solution Weight about 0.1 g (in dry basis; it shall make content of various sugar alcohol components within the scope of standard solution series, otherwise it can appropriately increase or reduce sample weight). Weigh it accurate to 0.0001 g. Use mobile phase to dissolve. Move it to 10 mL flask. Add mobile phase to make constant volume to scale. Use 0.22 µm membrane to filter the obtained solution. The filtrate is for later use. A.3.4.2 Standard curve drawing After use maltitol standard solution, sorbitol standard solution to respectively inject, take peak area of standard sample concentration to draw standard curve. The linear correlation coefficient shall be greater than 0.9990. A.3.5 Sample determination Inject well-prepared sample solution. Determine the nature of chromatographic peak of each sugar alcohol component in the sample according to retention time of standard product. Based on sample’s peak area, use external standard method or area normalization method (liquid) to calculate the percentage content of various sugar alcohols. A.3.6 Calculation result of external standard method Maltitol or sorbitol content is calculated by mass fraction of maltitol or sorbitol w1 (accounting for dry basis). The numerical value is represented by %. Calculate according to equation (A.1). Where, A1 - peak area of a maltitol in sample; ms - mass numerical value of a maltitol standard product in standard sample, in gram (g); Weigh a certain amount of sample in an iodine bottle. Add 25 mL of water. Slowly heat it to dissolve. Cool the solution. Add 20 mL of alkaline copper citrate test solution and some glass beads. After heating the solution for about 4 min, the solution starts to boil. Maintain the boiling for 3 min. After immediate cooling, add 100 mL of diluted acetic acid solution and 20.0 mL of 0.025 mol/L iodine titration solution. While shaking the solution, add 25 mL of diluted hydrochloric acid solution. After the precipitate is dissolved, use 0.05 mol/L sodium thiosulfate solution to titrate the excess iodine. When it is approaching to the end of the titration, add 2 mL of 1% starch indicator. The volume of consumed 0.05 mol/L sodium thiosulfate solution is less than 12.8 mL. A.5.4 Result determination A.5.4.1 Weigh 10 g (liquid product is calculated in dry basis) of sample. 0.05 mol/L sodium thiosulfate consumed by titration is not less than 12.8 mL. Standard of reducing sugar is ≤ 0.1%. A.5.4.2 Weigh 5 g (liquid product is calculated in dry basis) of sample. 0.05 mol/L sodium thiosulfate consumed by titration is not less than 12.8 mL. Standard of reducing sugar is ≤ 0.2%. A.5.4.3 Weigh 3.3 g (liquid product is calculated in dry basis) of sample. 0.05 mol/L sodium thiosulfate consumed by titration is not less than 12.8 mL. Standard of reducing sugar is ≤ 0.3%. A.6 Determination of residue on ignition A.6.1 Apparatus High temperature furnace. A.6.2 Reagents and materials Sulfuric acid. A.6.3 Analysis steps Weigh 1 g of sample (make it accurate to 0.0001 g). Put it into a porcelain crucible that has been burnt to constant weight. Slowly burn it on electric oven till complete carbonization. Cool it to room temperature. Add 0.5 mL of sulfuric acid to moisture it. Heat it at low temperature till sulfuric steam completely out. Then move it to high temperature furnace so as to burn it at 800°C±25°C to constant weigh. A.6.4 Result calculation Residue on ignition is calculated in mass fraction w3, numerical value in %. Where, α - angle of rotation determined, in degree (°); l - length of polarimeter tube, in decimeter (dm); ρa - mass concentration of maltitol in solution, in gram per milliliter (g/mL). A.8 Determination of sulfate. A.8.1 Regents and materials A.8.1.1 Hydrochloric acid solution. dilute 234 mL of hydrochloric acid to 1000 mL. A.8.1.2 Potassium sulfate standard solution. weigh 0.181 g of potassium; place in 1000 mL flask; add appropriate amount of water to dissolve it to scale; well shake it and the solution shall be obtained (1 mL corresponds to 100 µg of SO4). A.8.2 Apparatus A.8.2.1 Analytical balance. inductance is 0.01 g. A.8.2.2 50 mL Nessler colorimetric tube. A.8.3 Analysis steps Weigh about 1 g of sample in a 50 mL Nessler colorimetric tube. Add water and dissolve it to 40 mL. Add 2 mL of hydrochloric acid solution. Well shake it and sample solution shall be obtained. Take another 50 mL Nessler colorimetric tube. Add 1 mL of potassium sulfate standard solution. Add water to 40 mL. Add 2 mL of diluted hydrochloric acid. Well shake it and contrast solution shall be obtained. Respectively add 5 mL of 25% barium chloride solution into sample solution and contrast solution. Use water to dilute it to 50 mL. Fully shake them and place at black background for 10 min. Observe through Nessler colorimetric tube from top to bottom. Compare the turbidity generated. A.8.4 Result determination If turbidity of sample solution is less than contrast solution’s, it shall be determined as qualified. If turbidity of sample solution is greater than contrast solution’s, it shall be determined as unqualified. A.9 Determination of chloride A.9.1 Regents and materials ......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.