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GB 28307-2012 (GB28307-2012) PDF English
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GB 28307-2012: PDF in English

GB 28307-2012
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Additive - Maltitol and Maltitol Syrup
(Including No.1 “Amendment Sheet”, September 22, 2015)
ISSUED ON. APRIL 25, 2012
IMPLEMENTED ON. JUNE 25, 2012
Issued by. Ministry of Health of PRC
Table of Contents
1 Scope ... 3 
2 Chemical name, formula and relative molecular mass ... 3 
3 Product classification ... 3 
4 Technical requirements ... 4 
Appendix A ... 5 
National Food Safety Standard
Food Additive - Maltitol and Maltitol Syrup
1 Scope
This Standard applies to food additive - maltitol and maltitol syrup, which take
starch as raw material, are processed and manufactured by liquefaction,
saccharification, hydrogenation, refinement.
2 Chemical name, formula and relative molecular
mass
2.1 Chemical name
4-O-α-D- glucopyranosyl sorbitol.
2.2 Formula
C12H24O11.
2.3 Relative molecular mass
344.31 (in accordance with 2007 international relative atomic mass).
3 Product classification
3.1 Maltitol
3.1.1 Maltitol (category I)
High purity crystalline product.
3.1.2 Maltitol (category II)
Non-crystalline solid product directly dried by maltitol syrup.
3.2 Maltitol syrup
Liquid mixture composed by a large number of maltitol, a small amount of
sorbitol, oligosaccharide alcohol and polysaccharide alcohol.
Appendix A
Inspection methods
A.1 General rules
Reagents and water used in this Standard, in the absence of other requirements
specified, all refer to analytical reagents and grade three water stipulated in
GB/T 6682-2008. Standard solution used in test, standard solution,
preparations and products used in determination of impurities, in the absence
of other requirements specified, are prepared in accordance with provisions in
GB/T 601, GB/T 602, GB/T 603. Solution in test all refers to aqueous solution
when it does not indicate which solvent shall be used for preparation.
A.2 Identification test
A.2.1 Reagents and materials
A.2.1.1 Water. GB/T 6682-2008, grade one water.
A.2.1.2 Maltitol standard product. mass fraction ≥ 99.0%.
A.2.2 Apparatus
A.2.2.1 High performance liquid chromatography. equipped with refractive
index detector, or other equivalent detectors.
A.2.2.2 Mobile phase filtration vacuum degasser and 0.22 µm membrane.
A.2.2.3 Chromatographic column. specially used for analysis of sugars or
sugar alcohols which takes Ca type strong acid cation exchange resin as filler
(column size. Φ 7.8 mm × 300 mm), or equivalent chromatographic column.
A.2.3 Reference chromatographic conditions
A.2.3.1 Mobile phase. GB/T 6682-2008, grade one water.
A.2.3.2 Column temperature. 80°C.
A.2.3.3 Mobile phase flow rate. about 0.5 mL/min.
A.2.3.4 Injection volume. 20 µL.
A.2.4 Analysis steps
A.2.4.1 Preparation of standard solution
A.2.3.3 Mobile phase flow rate. about 0.5 mL/min.
A.2.3.4 Injection volume. 20 µL.
NOTE. System suitability is to repeat injecting standard solution twice. Relative error of
resulting response area is less than 20%.
A.3.4 Analysis steps
A.2.4.1 Preparation of sample solution
Weight about 0.1 g (in dry basis; it shall make content of various sugar alcohol
components within the scope of standard solution series, otherwise it can
appropriately increase or reduce sample weight). Weigh it accurate to 0.0001
g. Use mobile phase to dissolve. Move it to 10 mL flask. Add mobile phase to
make constant volume to scale. Use 0.22 µm membrane to filter the obtained
solution. The filtrate is for later use.
A.3.4.2 Standard curve drawing
After use maltitol standard solution, sorbitol standard solution to respectively
inject, take peak area of standard sample concentration to draw standard curve.
The linear correlation coefficient shall be greater than 0.9990.
A.3.5 Sample determination
Inject well-prepared sample solution. Determine the nature of chromatographic
peak of each sugar alcohol component in the sample according to retention
time of standard product. Based on sample’s peak area, use external standard
method or area normalization method (liquid) to calculate the percentage
content of various sugar alcohols.
A.3.6 Calculation result of external standard method
Maltitol or sorbitol content is calculated by mass fraction of maltitol or sorbitol
w1 (accounting for dry basis). The numerical value is represented by %.
Calculate according to equation (A.1).
Where,
A1 - peak area of a maltitol in sample;
ms - mass numerical value of a maltitol standard product in standard sample,
in gram (g);
Weigh a certain amount of sample in an iodine bottle. Add 25 mL of water.
Slowly heat it to dissolve. Cool the solution. Add 20 mL of alkaline copper citrate
test solution and some glass beads. After heating the solution for about 4 min,
the solution starts to boil. Maintain the boiling for 3 min. After immediate cooling,
add 100 mL of diluted acetic acid solution and 20.0 mL of 0.025 mol/L iodine
titration solution. While shaking the solution, add 25 mL of diluted hydrochloric
acid solution. After the precipitate is dissolved, use 0.05 mol/L sodium
thiosulfate solution to titrate the excess iodine. When it is approaching to the
end of the titration, add 2 mL of 1% starch indicator. The volume of consumed
0.05 mol/L sodium thiosulfate solution is less than 12.8 mL.
A.5.4 Result determination
A.5.4.1 Weigh 10 g (liquid product is calculated in dry basis) of sample. 0.05
mol/L sodium thiosulfate consumed by titration is not less than 12.8 mL.
Standard of reducing sugar is ≤ 0.1%.
A.5.4.2 Weigh 5 g (liquid product is calculated in dry basis) of sample. 0.05
mol/L sodium thiosulfate consumed by titration is not less than 12.8 mL.
Standard of reducing sugar is ≤ 0.2%.
A.5.4.3 Weigh 3.3 g (liquid product is calculated in dry basis) of sample. 0.05
mol/L sodium thiosulfate consumed by titration is not less than 12.8 mL.
Standard of reducing sugar is ≤ 0.3%.
A.6 Determination of residue on ignition
A.6.1 Apparatus
High temperature furnace.
A.6.2 Reagents and materials
Sulfuric acid.
A.6.3 Analysis steps
Weigh 1 g of sample (make it accurate to 0.0001 g). Put it into a porcelain
crucible that has been burnt to constant weight. Slowly burn it on electric oven
till complete carbonization. Cool it to room temperature. Add 0.5 mL of sulfuric
acid to moisture it. Heat it at low temperature till sulfuric steam completely out.
Then move it to high temperature furnace so as to burn it at 800°C±25°C to
constant weigh.
A.6.4 Result calculation
Residue on ignition is calculated in mass fraction w3, numerical value in %.
Where,
α - angle of rotation determined, in degree (°);
l - length of polarimeter tube, in decimeter (dm);
ρa - mass concentration of maltitol in solution, in gram per milliliter (g/mL).
A.8 Determination of sulfate.
A.8.1 Regents and materials
A.8.1.1 Hydrochloric acid solution. dilute 234 mL of hydrochloric acid to 1000
mL.
A.8.1.2 Potassium sulfate standard solution. weigh 0.181 g of potassium;
place in 1000 mL flask; add appropriate amount of water to dissolve it to scale;
well shake it and the solution shall be obtained (1 mL corresponds to 100 µg of
SO4).
A.8.2 Apparatus
A.8.2.1 Analytical balance. inductance is 0.01 g.
A.8.2.2 50 mL Nessler colorimetric tube.
A.8.3 Analysis steps
Weigh about 1 g of sample in a 50 mL Nessler colorimetric tube. Add water and
dissolve it to 40 mL. Add 2 mL of hydrochloric acid solution. Well shake it and
sample solution shall be obtained. Take another 50 mL Nessler colorimetric
tube. Add 1 mL of potassium sulfate standard solution. Add water to 40 mL. Add
2 mL of diluted hydrochloric acid. Well shake it and contrast solution shall be
obtained. Respectively add 5 mL of 25% barium chloride solution into sample
solution and contrast solution. Use water to dilute it to 50 mL. Fully shake them
and place at black background for 10 min. Observe through Nessler colorimetric
tube from top to bottom. Compare the turbidity generated.
A.8.4 Result determination
If turbidity of sample solution is less than contrast solution’s, it shall be
determined as qualified. If turbidity of sample solution is greater than contrast
solution’s, it shall be determined as unqualified.
A.9 Determination of chloride
A.9.1 Regents and materials
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.