GB 28401-2012 PDF in English
GB 28401-2012 (GB28401-2012) PDF English
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National food safety standards of food additives -- Phospholipid
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Standards related to (historical): GB 28401-2012
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GB 28401-2012: PDF in English GB 28401-2012
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard
Food Additives - Phospholipids
食品添加剂磷脂
ISSUED ON. MAY 17, 2014
IMPLEMENTED ON. JULY 17, 2012
Issued by. National Health and Family Planning Commission of the
People’s Republic of China
Table of Contents
1 Scope ... 3
2 Technical requirements ... 3
Appendix A ... 4
Appendix A
Test Methods
A.1 General
Unless otherwise specified, reagent and water in this Standard refer to analytic reagent
and Grade-3 water as specified in GB/T 6682-2008. Unless otherwise specified, standard
titration and reagent solutions used for determination of impurities shall be prepared in
accordance with GB/T601, GB/T 602 and GB/T 603. The solution used in test which is not
indicated with preparation method refers to aqueous solution.
A.2 Identification test
A.2.1 Put 2ml of ethanol solution (0.5%) in test tube; add 1 to 2 drops of cadmium chloride.
White precipitate is generated.
A.2.2 Put 2ml of ethanol solution (0.5%) in test tube; add 1 to 2 drops of bismuth
potassium nitrate solution (take 8g of bismuth nitrate; add 20ml of nitric acid to dissolve;
take 27.2 g of potassium iodide; add 50ml of water to dissolve; Mix the said two solutions;
add water to 100 ml). Brick-red precipitate is generated.
A.3 Test of insoluble substance — n-hexane
A.3.1 Reagent and substance
n-hexane.
A.3.2 Instruments
A.3.2.1 Suction filter. 500 ml.
A.3.2.2 Sand core glass crucible. G3.
A.3.3 Operation steps
A.3.3.1 Put a clean crucible in a 101°C ~ 105°C drying oven to bake to constant weight.
A.3.3.2 Take 10.0g of mixed samples (accuracy. 0.0001g); put it in beaker. Add 100ml of
n-hexane; stir the solution with glass rod to fully dissolve. Extract and filter through
constant-weight crucible.
A.3.3.3 Use total 25ml of n-hexane to clean beaker and glass rod for 2 times; transfer the
insoluble substances into crucible. Use n-hexane to wash the internal wall of crucible and
insoluble substances. Finally remove the residual n-hexane left inside crucible as
reductive or oxidizing substances. The surface of ground glass shall not be coated with
oil.
A.5.3 Operation steps
Take 5g of mixed samples (accurate to 0.001g); put it in a 250 mL iodine flask. Add 30ml
of mixed solution of glacial acetic acid and chloroform. Shake it to fully dissolve. Add 0.5ml
of saturated potassium iodide solution; put cover on it. Wait for 1min for reaction. At least
shake 3 times during the reaction process. Add 30ml of water and 0.5ml of starch indicator.
Use sodium thiosulfate standard titration solution to titrate the solution; keep shaking
during titration. When it is closing to end, keep shaking to make all of iodine released from
solvent layer. Add sodium thiosulfate standard titration solution drop-by-drop, until the
solution’s blue color fades away as the end. At the same time, conduct blank test. When
the consumed sodium thiosulfate standard titration solution exceeds 0.1ml for blank test, it
shall replace reagent and re-conduct the test.
A.5.4 Calculation
Peroxide value w3, the value is expressed by milligram equivalent of active oxygen per
kilogram meq/kg, is calculated according to Formula (A.3).
Where.
V — the volume of consumed sodium thiosulfate standard titration solution, unit. ml;
Vo — the volume of consumed sodium thiosulfate standard titration solution used in
blank test, unit. ml;
c — the accurate concentration of sodium thiosulfate standard titration solution, unit.
mol/L;
m3 — the mass of sample, unit. g;
1000 — conversion factor.
The arithmetic mean of the parallel determination results is the test result. The absolute
difference of two independent test results must not exceed 5% of the calculated arithmetic
mean, under repeatability conditions.
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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