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YC/T 249-2008: Tobacco and tobacco products. Determination of protein. Continuous flow method
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| YC/T 249-2008 | English | 169 |
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Tobacco and tobacco products. Determination of protein. Continuous flow method
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YC/T 249-2008
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Basic data | Standard ID | YC/T 249-2008 (YC/T249-2008) | | Description (Translated English) | Tobacco and tobacco products. Determination of protein. Continuous flow method | | Sector / Industry | Tobacco Industry Standard (Recommended) | | Classification of Chinese Standard | X87 | | Classification of International Standard | 65.160 | | Word Count Estimation | 6,693 | | Date of Issue | 2008-04-14 | | Date of Implementation | 2008-04-14 | | Issuing agency(ies) | State Tobacco Monopoly Administration | | Summary | This standard specifies the protein of tobacco and tobacco products in a continuous flow method. This standard applies to tobacco and tobacco products Determination of protein. | YC/T 249-2008: Tobacco and tobacco products. Determination of protein. Continuous flow method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Tobacco and tobacco products.Determination of protein.Continuous flow method
ICS 65.160
X87
Record Number. 23588-2008
People's Republic of China tobacco industry standards
Tobacco and tobacco products Determination of Protein
Continuous flow method
Posted 2008-04-14
2008-04-14 implementation
State Tobacco Monopoly Bureau issued
Foreword
This standard was proposed by the State Tobacco Monopoly Bureau.
This standard by the National Standardization Technical Committee on Tobacco (TC144) centralized.
This standard was drafted. Anhui tobacco industry.
The main drafters of this standard. the first Qu, Shu Junsheng, open Wang Bao, Cheng Tao, Jiang Shisheng, Tian Zhenfeng.
Tobacco and tobacco products Determination of Protein
Continuous flow method
1 Scope
This standard specifies the method for determination of the continuous flow of tobacco and tobacco products in the protein.
This standard applies to the determination of tobacco and tobacco products in the protein.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 5606.1 cigarette - Part 1. Sampling
General principles GB/T 19616 batches of raw tobacco samples (GB/T 19616-2004, ISO 4874.2000, MOD)
Preparation and water YC/T 31 samples of tobacco and tobacco products - Determination oven method
Determination of total nitrogen YC/T 161 Tobacco and tobacco products continuous flow method
Principle 3
Acetic acid solution was precipitated protein nitrogen in the sample. The samples were digested under alkaline conditions (pH 12.8 - 13.1), with a reagent solution
Online reaction liquid indigo compound, 660nm the indigo colorimetric determination in the compound.
4 Reagents and materials
4.1 Use of analytical reagent grade water should be distilled water or water of equivalent purity.
4.2 acetic acid solution, 0.5% (volume fraction).
4.3 Brij35 solution (polyethoxy lauryl ether)
250gBrij35 1000mL was added to the water and heated with stirring until dissolved.
4.4 concentrated sulfuric acid.
4.5 mercuric oxide (HgO), red.
4.6 potassium sulfate (K2SO4).
4.7 Sodium hypochlorite solution. Pipette 6mL sodium hypochlorite (chlorine content should not be less than 5%) in 100mL volumetric flask, water volume
To volume, the solution was added 2 drops of Brij35 (4.3).
4.8 sodium chloride/sulfuric acid solution. Weigh 10.0g of sodium chloride in a beaker, dissolved in water, was added 7.5mL of concentrated sulfuric acid (4.4), transferred to a
1000mL volumetric flask with water to volume, added 1mLBrij35 solution (4.3).
4.9 sodium salicylate/sodium nitroprusside solution. Weigh 75.0g of sodium salicylate (Na2C7H5O3), 0.15g sodium nitroprusside
[Na2Fe (CN) 5NO · 2H2O] in a beaker, dissolved in water, transferred to a 500mL volumetric flask with water to volume, added
0.5mLBrij35 solution (4.3).
4.10 buffer solution. Weigh 25.0g of potassium sodium tartrate (NaKC4H4O6 · 4H2O), 17.9g of disodium hydrogen phosphate (Na2HPO4 · 12H2O),
27.0g of sodium hydroxide (NaOH), dissolved in water, transferred to a 500mL volumetric flask with water to volume, add dissolved 0.5mLBrij35
Solution (4.3).
4.11 injector cleaning solution. Pipette 40mL of concentrated sulfuric acid (4.4) in 1000mL volumetric flask, slowly add water to the mark.
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