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SN/T 4603-2016: Detection of toxigenic gene of Vibrio parahaemolyticus in food and water for export - Multiplex PCR method and multiplex real-time PCR method
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Detection of toxigenic gene of Vibrio parahaemolyticus in food and water for export - Multiplex PCR method and multiplex real-time PCR method
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SN/T 4603-2016
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Basic data | Standard ID | SN/T 4603-2016 (SN/T4603-2016) | | Description (Translated English) | Detection of toxigenic gene of Vibrio parahaemolyticus in food and water for export - Multiplex PCR method and multiplex real-time PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 10,121 | | Date of Issue | 2016-08-23 | | Date of Implementation | 2017-03-01 | | Regulation (derived from) | State-Quality-Inspection-Certification (2016)438 | | Issuing agency(ies) | General Administration of Customs | SN/T 4603-2016: Detection of toxigenic gene of Vibrio parahaemolyticus in food and water for export - Multiplex PCR method and multiplex real-time PCR method
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Detection of toxigenic gene of Vibrio parahaemolyticus in food and water for export-Mutiplex PCR method and mutiplex real-time PCR method
People's Republic of China Exit Inspection and Quarantine standards
Export food and water producing Vibrio parahaemolyticus
Common pathogenic gene detection methods multiplex PCR and
Multiple real-time fluorescent PCR method
2016-08-23 released
2017-03-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
The "United States FDA Bacteriology Analysis Manual," Chapter Vibrio.2004 (Chapter9.Vibrio2004ofFDABacteriological
AnalyticalManual) and SN/T 2424-2010 "rapid detection of Vibrio parahaemolyticus in imported and exported food and real-time detection methods
Light PCR method "primer sequences combined together to form a multiplex PCR system.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted by Yunnan Entry-Exit Inspection and Quarantine of People's Republic of China, Shanghai Entry-Exit Inspection and Quarantine of the People's Republic of China
Bureau, Shanghai Jiaotong University, Shanghai Clinical Laboratory Center, Kunming Xishan District Center for Disease Control and Prevention.
The main drafters of this standard. Chen Liping, Liu Zhongmin, Zhang Jilun, Chen Yun, Zhang Jianhua, Shi Xianming, Wang Dapeng, Wang Qingzhong, Wang Jue.
Export food and water producing Vibrio parahaemolyticus
Common pathogenic gene detection methods multiplex PCR and
Multiple real-time fluorescent PCR method
1 Scope
This standard specifies the production of Vibrio parahaemolyticus multiplex PCR and multiple real-time fluorescent PCR detection method.
This standard applies to seafood, seafood condiments, water production in the detection of Vibrio parahaemolyticus common pathogenic bacteria genes.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
National Food Safety Standard Microbiological Examination of Food Vibrio parahaemolyticus test
GB/T 6682 analytical laboratory water specifications and test methods
GB 19489 laboratory biosafety common requirements
GB/T 27403 laboratory quality control specification food molecular biology test
The United States FDA Bacteriology Analysis Manual ninth chapter Vibrio.2004 (Chapter9.Vibrio2004ofFDABacteriological
AnalyticalManual)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Multiplex PCR mutiplexPCR
In the same reaction system by adding more than one pair of primers, while detecting several target gene PCR method.
3.2
Multiplex real-time PCR mutiplexreal-timePCR
In the same reaction system by adding more than one pair of primers, simultaneous detection of several target genes by real-time fluorescence PCR method.
4 Abbreviations
The following abbreviations apply to this document.
tdh. Heat-resistant hemolytic toxins corresponding gene (thermostabledirecthemolysin)
tlh. Heat-labile hemolytic toxin corresponding gene (thermolabilehemolysin)
trh. Heat-resistant direct hemolytic toxin-related hemolytic toxins corresponding gene (thermostablerelatedhemolysin)
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