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SN/T 4263-2015 English PDF

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SN/T 4263-2015: Determination of multi-appetite suppressants in food for export - LC-MS/MS method
Status: Valid
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Basic data

Standard ID SN/T 4263-2015 (SN/T4263-2015)
Description (Translated English) Determination of multi-appetite suppressants in food for export - LC-MS/MS method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Word Count Estimation 28,264
Date of Issue 2015-05-26
Date of Implementation 2016-01-01
Quoted Standard GB/T 6682
Regulation (derived from) State Quality-Inspection-Accreditation [2015] No.224
Issuing agency(ies) General Administration of Customs
Summary This standard provides the food for export fenfluramine, phenylpropanolamine, sibutramine, sertraline, bupropion, citalopram, fluoxetine, benfluorex liquid chromatography - mass spectrometry/MS detection and confirmation. This standard applies to tablets, capsules, powders, biscuits, oral solutions, coffee and tea in the eight kinds of appetite suppressants detection and confirmation.

SN/T 4263-2015: Determination of multi-appetite suppressants in food for export - LC-MS/MS method


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(Determination of the export of food in many appetite suppressant liquid chromatography - mass spectrometry/mass spectrometry) People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of food exports more appetite suppressant Liquid chromatography - mass spectrometry/mass spectrometry LC-MS/MSmethod Issued on. 2015-05-26 2016-01-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Chongqing, People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China Exit Inspection and Quarantine of Heilongjiang Province and the country. The main drafters of this standard. Ma Wei, Li Xiaojuan, Wang Jun Su, Liu Lei, on behalf of the Chinese Hui, Ma Qiang, Zhu informed, Wang Haibo, Hehao. Determination of food exports more appetite suppressant Liquid chromatography - mass spectrometry/mass spectrometry

1 Scope

This standard provides the food for export fenfluramine, phenylpropanolamine, sibutramine, sertraline, bupropion, citalopram, fluoxetine, benzene Fluoro-Ray Division LC - MS/MS detection and confirmation. This standard applies to tablets, capsules, powders, biscuits, oral solutions, coffee and tea in the eight kinds of appetite suppressants detection and confirmation.

2 Normative references

The following documents for the application of this document is essential. For dated references. Dated version only suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 Method summary

A sample of the eight kinds of appetite suppressants ultrasonic extraction with methanol solution with 0.1% formic acid - methanol mixture dilution; liquid chromatography - MS/MS detection, external standard.

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade water as a water GB/T 6682 regulations. 4.1 Methanol. HPLC grade. Acid 4.2. purity 99%, HPLC grade. 4.3 ammonium acetate. pure class distinctions. 4.4 10mmol/L ammonium acetate solution. Weigh 0.771g of ammonium acetate (4.3), dissolved in deionised water and diluted to 1000mL. 4.5 0.1% formic acid solution. Pipette 1.0mL of formic acid water volume to 1000mL. 4.6 0.1% formic acid - methanol mixture (37 volume). Pipette 30mL0.1% aqueous formic acid and 70mL of methanol, and mix. 4.7 Standard substance. fenfluramine, phenylpropanolamine, sibutramine, sertraline, bupropion, citalopram, fluoxetine, benfluorex Standard. Purity of greater than or equal to 95%, see Appendix A. 4.8 standard stock solution. Weigh 0.01g (accurate to 0.0001g) standard product, in addition to a small amount of sertraline dissolved in water, with methanol Yung, other standards are dissolved in methanol and dilute to 100mL, dubbed the concentration of 100.0mg/L standard stock solution, and placed in -18 ℃ insurance Deposit, valid for three months. 4.9 intermediate mixed standard solution. Pipette appropriate amount of eight kinds of appetite suppressants stock standard solution in 100mL flask, constant volume with methanol To the mark, dubbed hybrid intermediate standard solution. So mixed standard working solution fenfluramine 250ng/mL, phenylpropanolamine 500ng/mL, Sibutramine 100ng/mL, sertraline 500ng/mL, bupropion 500ng/mL, citalopram 1000ng/mL, fluoxetine 1000ng/mL, benfluorex 500ng/mL. Placed in -18 ℃ preservation, valid for 1 month. 4.10 mixed standard working solution. methanol mixed intermediate standard solution (4.9) stepwise diluted to the appropriate concentration of mixed standard required Working solution, to be using now. 4.11 plastic centrifuge tube. 50mL, stoppered.

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