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SN/T 4050-2014: Quarantine Protocol for infection with abalone herpesvirus
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| Std ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status |
| SN/T 4050-2014 | English | 389 |
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Quarantine Protocol for infection with abalone herpesvirus
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Basic data
| Standard ID | SN/T 4050-2014 (SN/T4050-2014) |
| Description (Translated English) | Quarantine Protocol for infection with abalone herpesvirus |
| Sector / Industry | Commodity Inspection Standard (Recommended) |
| Classification of Chinese Standard | B41 |
| Classification of International Standard | 07.080 |
| Word Count Estimation | 15,138 |
| Date of Issue | 12/1/2014 |
| Date of Implementation | 5/1/2015 |
| Quoted Standard | GB/T 6682; GB/T 18088; GB 19489; SN/T 1193 |
| Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 |
| Issuing agency(ies) | General Administration of Customs |
| Summary | This standard specifies the clinical diagnosis, histopathology and molecular biology methods abalone herpes virus infection. This standard applies to abalone herpes virus infection diagnosis and monitoring. |
SN/T 4050-2014: Quarantine Protocol for infection with abalone herpesvirus
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Abalone herpes virus infection quarantine technical specifications)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Abalone herpes virus infection quarantine Technical Specifications
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shandong Entry-Exit Inspection and Quarantine Yantai People's Republic of China
Bureau, the Shenzhen People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Zhao Ran, Yin Wei force, Jiang Yuting, Zhengxiao Cong, Sun Mingjun, Zheng Xiaolong, He Junqiang, in force, Wang Jinjin,
Yue Zhiqin.
Abalone herpes virus infection quarantine Technical Specifications
1 Scope
This standard specifies the clinical diagnosis, histopathology and molecular biology methods abalone herpes virus infection.
This standard applies to abalone herpes virus infection diagnosis and monitoring.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
GB 19489 General requirements for laboratory biosafety
SN/T 1193 genetic testing laboratory technical requirements
3 Abbreviations
The following abbreviations apply to this document.
AbHV. abalone herpesvirus (Abaloneherpesvirus)
BCIP. 5- bromo-4-chloro-3-indolyl - phosphate (5-Bromo-4-Chloro-3-IndolylPhosphate)
Ct value. Threshold fluorescence signal reaches the set number of cycles experienced by amplification (Cyclethresholdvalue)
EDTA. ethylenediaminetetraacetic acid (EthyleneDiamineTetraaceticAcid)
NBT. nitroblue tetrazolium (NitrotetrazoliumBluechloride)
PCR. polymerase chain reaction (Polymerasechainreaction)
SDS. sodium dodecyl sulfate (Sodiumdodecylsulfate)
4 Reagents and materials
4.1 Water. in line with GB/T 6682 in the three water specifications.
4.2 hematoxylin.
4.3 eosin.
4.4 Primer. Table 1.
Table 1 Primer sequences
Name Sequence
AbHVORF66F1 5'-TCC-CGG-ACA-CCA-GTA-AGA-AC-3 '
AbHVORF66R1 5'-CAA-GGC-TGC-TAT-GCG-TAT-GA-3 '
AbHV66Prb1 5'-6FAM-TGG-CCG-TCG-AGA-TGT-CCA-TG-TAMRA-3 '
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