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SN/T 4052-2014: Determination of nuciferine in health foods for export
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4052-2014 | English | 289 |
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Determination of nuciferine in health foods for export
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SN/T 4052-2014
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Standard similar to SN/T 4052-2014 SN/T 5268 Basic data | Standard ID | SN/T 4052-2014 (SN/T4052-2014) | | Description (Translated English) | Determination of nuciferine in health foods for export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 11,176 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies two methods for determining nuciferine in health foods, namely liquid chromatography - mass spectrometry/mass spectrometry and HPLC. This standard applies to lipid capsules, chewable tablets to lose weight and weight loss tea nucif | SN/T 4052-2014: Determination of nuciferine in health foods for export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Determination of export health foods nuciferine)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of health food exports in nuciferine
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hunan Exit Inspection and Quarantine, Hunan Inspection and Quarantine Institute of Science and Technology.
The main drafters of this standard. Jiaoyan Na, Yan Hongfei, Fushan Liang, Wang Meiling, Li Yongjun, Zhang Ying, Huang Zhiqiang.
Determination of health food exports in nuciferine
1 Scope
This standard specifies two methods for determining nuciferine in health foods, namely liquid chromatography - mass spectrometry/mass spectrometry and HPLC.
This standard applies to lipid capsules, chewable tablets to lose weight and weight loss tea nuciferine quantitative determination and confirmation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
The first method by liquid chromatography - mass spectrometry/mass spectrometry
Principle 3
The sample nuciferine ultrasonic extraction with methanol, the extract was diluted back injector, high performance liquid chromatography - mass spectrometry/mass spectrometer, external standard method
Quantified.
4 Reagents and materials
Except where noted, all reagents were of analytical grade water as a water line with GB/T 6682 regulations.
4.1 Methanol. HPLC grade.
4.2 acetonitrile. HPLC grade.
4.3 acetic acid.
4.4 0.1% acetic acid solution. accurately Pipette 1mL acetic acid (4.3), diluted with water to 1000mL.
4.5 methanol - water mixed solution (11 volume). Measure 100mL methanol, 100mL water was added, mixing spare.
4.6 nuciferine Standards (CAS Number. 475-83-2 molecular formula. C19H21NO2). purity ≥99%.
4.7 nuciferine stock standard solution. Accurately weigh an adequate amount nuciferine standards, dissolved in methanol to prepare a concentration of 1.0mg/mL standard
Quasi stock solution, 0 ℃ ~ 4 ℃ stored in the dark.
4.8 nuciferine standard solution. Standard stock solution Pipette accurately a volume of methanol necessary - dilution water mixed solution (4.5)
Standard working solution with different concentrations of the series.
5 Instruments
5.1 High performance liquid chromatography - mass spectrometry/mass spectrometry. Distribution ionization (ESI).
5.2 ultrasound.
5.3 mill.
5.4 Analysis of balance. a sense of the amount of 0.0001g and 0.01g.
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