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SN/T 3740-2013: Detection method for St Louis encephalitis virus at frontier ports. Real-time fluorescence TR-PCR
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Detection method for St Louis encephalitis virus at frontier ports. Real-time fluorescence TR-PCR
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SN/T 3740-2013
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Basic data | Standard ID | SN/T 3740-2013 (SN/T3740-2013) | | Description (Translated English) | Detection method for St Louis encephalitis virus at frontier ports. Real-time fluorescence TR-PCR | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 11.020 | | Word Count Estimation | 8,815 | | Quoted Standard | GB 19489; WS 233 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the frontier port of St. Louis encephalitis virus nucleic acid fluorescence in real-time RT-PCR method to detect infection or suspected cases of St. Louis encephalitis mosquito carrying the virus, including specimen collection, sto | SN/T 3740-2013: Detection method for St Louis encephalitis virus at frontier ports. Real-time fluorescence TR-PCR
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Detection method for St Louis encephalitis virus at frontier ports. Real-time fluorescence TR-PCR
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Frontier Port St. Louis encephalitis virus detection methods
Real-time fluorescent RT-PCR method
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chinese Academy of Inspection and Quarantine, Shenzhen People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. new hole Hu Shi Lei, Yang Pengfei, Yanqing Li, Zhang Liping, Xu Huanzhou, Sun Xiaohong, Liu Lijuan, Yang Yu, Wang Jing.
Frontier Port St. Louis encephalitis virus detection methods
Real-time fluorescent RT-PCR method
1 Scope
This standard specifies the border crossings St. Louis encephalitis virus infection or suspected cases of St. Louis encephalitis mosquito carrying the virus nucleic acid in real-time fluorescence
Light RT-PCR detection methods, including specimen collection, storage and transport, sample processing, detection, and determines and reports.
This standard applies to border crossings infected or suspected cases of St. Louis encephalitis virus in mosquitoes carrying St. Louis encephalitis virus lab
Testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
WS233 Microbiological and Biomedical Laboratories Biosafety Common Criteria
Can infect humans highly pathogenic microorganisms (viruses) or sample transport regulations (People's Republic of China Ministry of Health Order
No. 45)
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
St. Louis encephalitis virus St.Louisencephalitisvirus
Genus Flaviviridae (Flaviviridae) Flavivirus (Flavivirus) single-strand RNA virus, and Japanese encephalitis virus, West Nile disease
Poison, Murray Valley encephalitis virus (MurrayValeyEncephalitisvirus), Kunjin virus (Kunjunvirus), etc. belong to one serotype,
It is an insect-borne virus, mainly through Culex (determined in US media to mosquitoes Culextarsalis, Cx.pipiens, Cx.quinque-
fasciatus and Cx.nigripalpus based) transmission, as the main host of birds to the bird - mosquito - bird cycle as the basic transmission cycle. Humanity
Is currently the only known species after species naturally infected with the pathogenic virus, the virus infection in humans can cause fever, headache and other flu-like symptoms
Like, also showed symptoms of central nervous system infection, severe cases may die.
4 detects objects
Frontier Port St. Louis encephalitis virus infection or suspected cases intercepted mosquitoes.
5 laboratory bio-safety requirements
5.1 CHECK personal protective reference GB 19489.
5.2 Biosecurity should comply with GB 19489 and WS233 for biosafety level 2 (BSL-2) is required for biosafety laboratories.
5.3 Used Lab Supplies shall comply with GB 19489 requirements for the processing of waste treatment and disposal.
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