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SN/T 3736-2013: Detection method for enterovirus 71(EV71 virus)by real time fluorescence RT-PCR at frontier ports
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Detection method for enterovirus 71(EV71 virus)by real time fluorescence RT-PCR at frontier ports
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SN/T 3736-2013
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Basic data | Standard ID | SN/T 3736-2013 (SN/T3736-2013) | | Description (Translated English) | Detection method for enterovirus 71(EV71 virus)by real time fluorescence RT-PCR at frontier ports | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 11.020 | | Word Count Estimation | 8,890 | | Quoted Standard | GB 19489; WS 233 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies requirements for border crossings Biosafety EV71 HFMD virus detection, specimen collection, transport and storage, handling, EV71 virus real-time fluorescence detection method RT- PCR samples. This standard applies to people abroad | SN/T 3736-2013: Detection method for enterovirus 71(EV71 virus)by real time fluorescence RT-PCR at frontier ports
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Detection method for enterovirus 71 (EV71 virus) by real time fluorescence RT-PCR at frontier ports
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Border crossings enterovirus 71 (EV71 virus)
Real-time fluorescent RT-PCR detection method
Detectionmethodforenterovirus71 (EV71virus) byrealtime
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China, Shenzhen CIQ, Shenzhen Inspection and Quarantine Science Research Institute, the Chinese people
Republic of Sichuan Exit Inspection and Quarantine.
The main drafters of this standard. Liu Sheng teeth, Zhu Yulan, Tianlv Bo, Gu Li Bing, Fan Xuejun, Xiecong Xian, Chen Sheng west, Zhou Lian Zhi, Zeng Chuan-ping,
Sun Dong body, Zhangni Qi, Shi Ying.
Border crossings enterovirus 71 (EV71 virus)
Real-time fluorescent RT-PCR detection method
1 Scope
This standard specifies the requirements for biosafety border crossings EV71 HFMD virus detection, specimen collection, transport and preservation of specimens
Processing, EV71 virus real-time fluorescent RT-PCR detection method.
This standard applies to molecular biology frontier ports of entry and exit who carry EV71 virus.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
WS233 Microbiological and Biomedical Laboratories Biosafety Common Criteria
It can infect humans highly pathogenic microorganisms (viruses) or sample transport regulations (People's Republic of China Ministry of Health Decree
No. 45)
Pathogenic microorganisms (viruses) Collection agency management approach (Ministry of Health) Human infection
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Enterovirus 71 Enterovirus71
One of the main pathogens causing HFMD. Are small RNA virus family (picornaviridae) of enterovirus (entero-
virus), A group of enteroviruses. EV71 virus is a single positive-stranded RNA, non-enveloped viruses, small spherical (diameter 20nm ~ 30nm), 20 surface
Body symmetry.
4 Abbreviations
The following abbreviations apply to this document
RT-PCR. reverse transcriptase polymerase chain reaction (Reversetranscription-polymerasechainreaction)
Cycles (Cyclethreshold) experienced by threshold cycle, each reaction tube of the fluorescent signal reaches a set threshold when. Ct value
RNA. ribonucleic acid (Ribonucleicacid)
FAM. 6- carboxy-fluorescein, a fluorescent reporter group (6-carboxy-fluorescein)
TAMARA. 6- carboxy-tetramethylrhodamine a fluorescent quencher (6-carboxytetramethylrhodamine)
5 bio-safety requirements
5.1 Personal Protective follow GB 19489.
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