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SN/T 3739-2013: Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR
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Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR
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SN/T 3739-2013
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Basic data | Standard ID | SN/T 3739-2013 (SN/T3739-2013) | | Description (Translated English) | Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C62 | | Classification of International Standard | 11.020 | | Word Count Estimation | 12,12 | | Quoted Standard | List of human infectious pathogens | | Adopted Standard | List of human infectious pathogens | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the border exit port personnel or tick-borne encephalitis virus carrying real-time fluorescent RT-PCR detection, including detection of objects, specimen collection and handling, testing procedures, the results of the determination | SN/T 3739-2013: Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR
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Detection method for tick-borne encephalitis virus at frontier ports. Real-time fluorescence RT-PCR
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Border crossings tick-borne encephalitis virus detection methods
Real-time fluorescent RT-PCR method
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Entry-Exit Inspection and Quarantine of Jilin People's Republic of China
Bureau of Shanghai Jiang Biological Technology Co., Ltd..
The main drafters of this standard. Li Zhihui, Zhan Xi Jing, Daniel Yeung, Yang Shunyi, sinus Yong Ying, Liu Yang, Zhuxu Ping, Zhu Qin Wei.
Border crossings tick-borne encephalitis virus detection methods
Real-time fluorescent RT-PCR method
1 Scope
This standard specifies the border ports of entry and exit personnel or tick-borne encephalitis virus carrying real-time fluorescent RT-PCR detection, including the detection of
Like, specimen collection and handling, testing procedures, the results of determination.
This standard applies to the border port of entry and exit personnel or tick-borne encephalitis virus carrying laboratory tests.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Human infection of pathogenic microorganisms directory (Department of Health 2006)
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Tick-borne encephalitis virus tick-borneencephalitisvirus
Russian spring-summer encephalitis virus Russianspring-summerencephalitisvirus
Flaviviridae Flavivirus member, single-strand RNA viruses, mRNA full length 10886bp. The virus occurs According to school system
Europe is divided into subtypes, the Far East and Siberia subtype subtypes three subtypes. The virus caused encephalitis is a natural focal disease.
The virus reservoir host of many, the media mainly ticks, usually caused by susceptible people carrying the virus after being bitten by a tick disease.
3.2
Real-time fluorescent RT-PCR real-timeRT-PCR
There are a variety of real-time fluorescent RT-PCR method, this standard uses a TaqMan hydrolysis probe method, the principle is the conventional RT-PCR
On the basis of adding a specific fluorescent probe which is an oligonucleotide, the two sides were marked with a fluorescent reporter group and a
A fluorescence quenching group. When the probe is intact, the fluorescence signal emitted by the reporter group is absorbed by the quencher, PCR amplification using Taq enzyme
5'-3 'exonuclease activity of the enzyme hydrolysis probes, the fluorescent reporter group and fluorescence quenching group separation, the fluorescence monitoring system can receive
The fluorescence signal. Each through a PCR cycle, the fluorescence signal is also the same fragment, there is a coincident index increased process signals
Nucleic acid content is proportional to the intensity of the detected object, it can calculate the amount of detected objects according to a mathematical model.
4 Abbreviations
The following abbreviations apply to this document.
BHQ. black hole quencher (blackholequencher)
Ct values. cycle threshold values, each reaction tube fluorescent signal cycle number (cyclethreshold) set threshold experienced
DEPC. diethyl pyrocarbonate (diethylpyrocarbonate)
FAM. 6- carboxy-fluorescein, a fluorescent reporter group (6-carboxy-fluorescein)
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