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SN/T 2668-2010: Event-specific method for the detection of genetically modified plants
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| SN/T 2668-2010 | English | 369 |
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Event-specific method for the detection of genetically modified plants
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SN/T 2668-2010
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Basic data | Standard ID | SN/T 2668-2010 (SN/T2668-2010) | | Description (Translated English) | Event-specific method for the detection of genetically modified plants | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.040 | | Word Count Estimation | 14,155 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682; SN/T 1193; SN/T 1194 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies several important transgenic plant lines specificity (transformation event-specific) detection methods. This standard applies to genetically modified corn (Btl0, Btll, GA21, Mon810, Mon863. NK603, T25, TCl507), genetically modified soybean (GTS-40-3-2, A2704-12), genetically modified oilseed rape (RT73. T43. Ms8. Rf3), transgenic tomato (China Fan No. l) and transgenic potato (EH92-527-1) strains specific detection. | SN/T 2668-2010: Event-specific method for the detection of genetically modified plants---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Event-specific method for the detection of genetically modified plants
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Transgenic plant lines specific detection method
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Xiamen People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
Exit Inspection and Quarantine and the State Beijing, People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Liaoning Entry-Exit Inspection
Quarantine Bureau.
The main drafters of this standard. Chen Yun, Liang Miao, Chen Shuangya, Zhao Wenjun, Huang Wensheng, Cao occasion Juan Pan Liangwen, water Zhu Fang, Chen Hongjun.
Transgenic plant lines specific detection method
1 Scope
This standard specifies several important transgenic plant lines specificity (transformation event specific) detection method.
This standard applies to genetically modified maize (Bt10, Bt11, GA21, Mon810, Mon863, NK603, T25, TC1507), genetically modified
Beans (GTS-40-3-2, A2704-12), transgenic rapeseed (RT73, T45, Ms8, Rf3), transgenic tomato (Hua Fan No. 1) and transgenic potato
Potato (EH92-527-1) of strain-specific detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1193 genetic testing laboratory technical requirements
Detection sampling and sample preparation method SN/T 1194 in genetically modified plants and their products
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
3.1.1
Transgenic plants geneticalymodifiedplant
Gene of interest (including exogenous and endogenous gene categories) introduced into the plant genome and stably inherited in progeny plants.
3.1.2
Transgenic plant lines geneticalymodifiedplantevent
The success of the target gene can be introduced into the plant genome and in the offspring that is genetically stable transgenic plant lines, except the same gene transfer
Of the body as a different strain. Strain-specific sequence refers to an exogenous sequence fragment inserted into the connection area and the plant genome, transgenic plant products
Identification based system.
3.2 Acronyms
The following abbreviations apply to this document.
Adh1 (alcoholdehydrogenase). alcohol dehydrogenase gene, maize endogenous gene
bp (basepair). bp
Ct values (Cyclethreshold). each reaction tube fluorescent signal reaches a set threshold number of cycles experienced by
CruA (CruciferinAgene). canola seed storage proteins, rape endogenous gene
dATP (deoxyadenosinetriphosphate). triphosphate
dCTP (deoxycytidinetriphosphate). deoxycytidine triphosphate
dGTP (deoxyguanosinetriphosphate). deoxyguanosine triphosphate
DNA (deoxyribonucleicacid). DNA
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