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SN/T 2667-2010: Qualitative detection for genetically modified microorganisms
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| SN/T 2667-2010 | English | 239 |
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Qualitative detection for genetically modified microorganisms
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SN/T 2667-2010
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Basic data | Standard ID | SN/T 2667-2010 (SN/T2667-2010) | | Description (Translated English) | Qualitative detection for genetically modified microorganisms | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 07.100.01 | | Word Count Estimation | 9,954 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682; SN/T 1193 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the qualitative detection of genetically modified micro-method. This standard applies to portable biochemical marker genes color, light gene, ampicillin and kanamycin resistance gene qualitative detection of genetically modified micro-organisms. | SN/T 2667-2010: Qualitative detection for genetically modified microorganisms---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Qualitative detection for genetically modified microorganisms
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Qualitative detection of genetically modified micro-organisms
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Xiamen People's Republic of China Exit Inspection and Quarantine, the People's Republic of China
Exit Inspection and Quarantine and the State Beijing.
The main drafters of this standard. Liang Miao, Chen Yun, Zhao Wenjun, Chen Shuangya, water Zhu Fang, Chen Hongjun.
Qualitative detection of genetically modified micro-organisms
1 Scope
This standard specifies the qualitative detection of genetically modified micro-organisms.
This standard applies to carry genetically modified microorganisms and biochemical chromogenic marker genes, luminescent enzyme gene, ampicillin and kanamycin resistance gene
Material qualitative detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1193 genetic testing laboratory technical requirements
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
3.1.1
GM microorganisms geneticalymodifiedmicroorganisms; GMM
By gene manipulation techniques of genetic material (DNA) restructuring, modification, thereby changing the configuration of microbial genomes.
3.2 Acronyms
The following abbreviations apply to this document.
GFP (greenfluorescentprotein). green fluorescent protein
gusA (β-glucuronidase). β- glucuronidase gene
LacZ. β- galactosidase gene
Luc. luciferase gene in eukaryotes
LuxAB. bacterial luciferase gene
X-gal (5-bromo-4-chloro-3-indoyl-β-Dgalactopyranoside). 5- bromo-4-chloro-3-indolyl galactoside -β-
X-GlcA (5-bromo-4-chloro-3-indoyl-β-D-glucuronide). 5- bromo-4-chloro-3-indolyl glucuronide -β-
xylE (catechol2,3-dioxygenase). catechol 2,3-dioxygenase gene
Principle 4
4.1 Selective culture
Selective culture as a simple and sensitive method for detection of genetically modified microorganisms. Containing some kind of suppression of naturally occurring micro
Population of the compound. (such as antibiotics, heavy metals, etc.) media, selective cultivation of genetically modified micro-organisms. According GMM
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