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SN/T 2664-2010: Determination of tetracyclines residues in royal jelly for import and export. Radio-receptor assay method
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Determination of tetracyclines residues in royal jelly for import and export. Radio-receptor assay method
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SN/T 2664-2010
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Basic data | Standard ID | SN/T 2664-2010 (SN/T2664-2010) | | Description (Translated English) | Determination of tetracyclines residues in royal jelly for import and export. Radio-receptor assay method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 13,110 | | Date of Issue | 2010-11-01 | | Date of Implementation | 2011-05-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | National Quality Inspection (2010) 582 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the tetracyclines residues in royal jelly radioactive receptor assays (charm ��) method. This standard applies to royal jelly tetracycline, chlortetracycline, oxytetracycline, doxycycline antibiotic residues in the determination of the total amount. | SN/T 2664-2010: Determination of tetracyclines residues in royal jelly for import and export. Radio-receptor assay method
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Determination of tetracyclines residues in royal jelly for import and export.Radio-receptor assay method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Royal Jelly tetracyclines Residues
Determination radioreceptor assay
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters. Zhang Xiaofeng, Chen Xiaomei, Zhu Zhenjiang, Shi Weiliang, Fang Ying.
Royal Jelly tetracyclines Residues
Determination radioreceptor assay
1 Scope
This standard specifies the royal jelly tetracycline antibiotic residues in radioreceptor assay (CharmⅡ) method.
This standard applies to tetracycline, chlortetracycline, oxytetracycline, doxycycline measured total antibiotic residues in royal jelly.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
Radioreceptor assay based (CharmⅡ) method is a combination of functional group reactions to drugs and microbial receptor sites, these sites with a certain type
There are antibiotics related functional groups. This application-specific receptor can achieve multi-residue analysis of a class of antibiotics. This method
The sample remaining in the tetracycline class of drugs was extracted and diluted with [3H] labeled chlortetracycline common competition binding specific receptor sites. In a
After a given reaction temperature, centrifugation, remove unbound tetracyclines, finally adding scintillation fluid to measure [3H] β decay particles emitted put
Radioactive measure, count cpm (countperminute). Inversely proportional to the tetracycline antibiotics residues in cpm values measured in the sample.
4 Reagents and materials
Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.
4.1 Tetracycline Assay Kit 1).
4.1.1 Organization negative control concentrated powder. negative control tissue is concentrated dry powder stored at 2 ℃ ~ 6 ℃.
4.1.2 MSU multi-resistant standards powder concentrate. concentrated powder stored at 2 ℃ ~ 6 ℃.
4.1.3 Organization extraction buffer (MSU extraction buffer solution) powder. Store at 2 ℃ ~ 6 ℃.
4.1.4 M2 buffer solution powder. used to adjust the pH value, stored in 2 ℃ ~ 6 ℃.
4.1.5 tetracycline receptor tablets. white, under conditions of -15 ℃ to save.
4.1.6 tritiated tetracycline tablets. Orange, the following conditions -15 ℃ preservation.
4.2 Organization negative control solution. Take the negative control tissue concentrate powder (4.1.1) when used according to kit instructions formulated as a negative control solution
liquid. Good solution preparation can be stored at 2 ℃ ~ 6 ℃ refrigerator or ice bath 48h. Such a long time, the solution may be stored at -15 ℃ to
Under the refrigerator, when used to thaw. Then thawed solution may be stored at 2 ℃ ~ 6 ℃ refrigerator for 24h.
4.3 MSU multi-resistant standard solution. take when using concentrated powder (4.1.2) according to kit instructions formulated as a multi-standard antibiotic solution. Dissolve
The method of preservation solution with 4.2.
1) This information is given for the convenience of users of this standard does not imply endorsement of a particular product. If other product has the same effect, subject to real
Using these products is equivalent to a posteriori assessment.
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