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SN/T 2530-2010: Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2530-2010 | English | 369 |
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Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR
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SN/T 2530-2010
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Basic data | Standard ID | SN/T 2530-2010 (SN/T2530-2010) | | Description (Translated English) | Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 14,149 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Quoted Standard | SN/T 1193 | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the shellfish, fruits and vegetables and water samples poliovirus ordinary and real-time RT-PCR and fluorescent RT-RCR method. This standard applies to shellfish, fruits and vegetables and water samples in the qualitative detection of poliovirus. | SN/T 2530-2010: Determination of polioviruses in shellfish, fruit, vegetable and water. Conventional RT-PCR and real-time RT-PCR
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Determination of polioviruses in shellfish, fruit, vegetable and water.Conventional RT-PCR and real-time RT-PCR
Exit inspection and quarantine industry standard book People's Republic of China
Shellfish, fruits and vegetables and water samples poliovirus
Detection by RT-PCR and common
Real-time fluorescent RT-PCR.
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Xiang, Panliang Wen, Zhong Shan Lu, Lu Rong, Zhang Shuya, Huang, Liu Yueming, high piano.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Shellfish, fruits and vegetables and water samples poliovirus
Detection by RT-PCR and common
Real-time fluorescent RT-PCR.
1 Scope
This standard specifies the shellfish, fruits and vegetables and water samples poliovirus ordinary by RT-PCR and real-time RT-PCR method.
This standard applies to qualitative detection of shellfish, fruits and vegetables and water samples poliovirus.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
SN/T 1193 genetic testing laboratory technical requirements
3 Terms and Definitions
The following terms and definitions apply to this standard.
3.1
When the number of cycles of each reaction tube fluorescent signal reaches the set value of the field experienced.
3.2
Recombinant molecule comprising a virus-specific cDNA fragments can be detected as a virus PCR positive control.
4 Method summary
Viral RNA was extracted shellfish sample with a suitable lysis buffer (such as Tri-reagent), and in accordance with poliovirus RNA3 'end
Containing Poly (A) structure, connecting with Oligo (dT) 25 magnetic beads specific adsorption of poliovirus RNA was purified. Water samples
The virus enrichment, using the appropriate method for the extraction and purification of viral RNA. Eluted with a suitable buffer virus sample surface of fruits and vegetables
After the virus enrichment, and use the appropriate method for the extraction and purification of viral RNA. Use ordinary fluorescent RT-PCR or real-time RT-PCR
Methods for testing. In this study, by constructing a plasmid standard molecules (plasmid molecule contains one copy of each amplified fragment), to determine the suitable type Ⅰ,
Ⅱ type, Ⅲ type polio virus detection system common RT-PCR detection limit were 50 copies, real-time fluorescent RT-PCR detection system
Measurement limit is 2 copies.
5 Reagents
All experimental reagents were of analytical grade; unless otherwise stated, the test water is distilled or deionized water.
5.1 positive samples. poliovirus, -80 ℃ refrigerator; or containing polio virus detection fragment of the plasmid standard score
Son, -20 ℃ refrigerator.
5.2 glycine buffer. see A. 1.1.
5.3 PEG8000 solution. see A. 1.2.
5.4 PBS buffer. see A. 1.3.
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