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SN/T 2528-2010: Determination of Legionella in drinking water
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2528-2010 | English | 259 |
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Determination of Legionella in drinking water
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SN/T 2528-2010
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Basic data | Standard ID | SN/T 2528-2010 (SN/T2528-2010) | | Description (Translated English) | Determination of Legionella in drinking water | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 10,156 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Quoted Standard | GB/T 27403; GB/T 27405; SN/T 1538.1; SN/T 1538.2 | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the detection of Legionella in drinking water method. This standard applies to the detection of Legionella in drinking water. Industrial water and natural water and sediment, sediment and clay/ooze and other related subjects testing for legionella samples refer to this method. | SN/T 2528-2010: Determination of Legionella in drinking water---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of Legionella in drinking water
Exit inspection and quarantine industry standard book People's Republic of China
Legionella detected in drinking water
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix, Appendix B to Appendix informative.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Entry-Exit Inspection and Shanxi
Quarantine Bureau.
The main drafters of this standard. Li Xiaohong, Li Weihua, Han Wei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Legionella detected in drinking water
1 Scope
This standard specifies the method for the detection of Legionella in drinking water.
This standard is applicable to the detection of Legionella bacteria in drinking water. Industrial water and natural water and sediment, sediment and clay/ooze and other related
Samples for legionella can refer to this method.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 27403 laboratory quality control of food molecular biology standardized testing
GB/T 27405 laboratory quality control standard microbiological testing of food
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Definitions and Terminology
The following terms and definitions apply to this standard.
3.1
The bacteria are rounded at both ends, with end flagella, dynamic, non-spore and capsule, aerobic and facultative anaerobic size (0.3μm ~
0.4μm) × (2.0μm ~ 3.0μm) or (0.5μm ~ 0.7μm) × (2.0μm ~ 20μm) Gram-negative bacilli.
4 Equipment and Materials
4.1 Incubator. 36 ℃ ± 1 ℃.
4.2 straw. 1mL, 5mL and 10mL, sub-scale 0.1mL.
4.3 inoculating loop. diameter 3mm.
4.4 Balance. a sense of the amount of 0.1g.
4.5 vacuum filtration system.
4.6 sterilized dishes. pan base diameter 9cm.
4.7 turbidity.
4.8 PCR amplification.
4.9 desktop centrifuge. highest centrifugal force 13000r/min.
4.10 low magnification binocular microscope.
4.11 temperature water bath. 36 ℃ ± 1 ℃.
4.12 Black nitrocellulose membranes. a diameter of 47mm ~ 50mm, pore size 0.45μm. Milipore or similar products.
4.13 micro-adjustable pipette. 1μL ~ 10μL, 100μL, 200μL, 1000μL.
4.14 Vortex.
4.15 electrophoresis.
4.16 gel imaging analysis system.
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