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Determination of sterigmatocystin contents in cereals for import and export. HPLC method
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SN/T 2483-2010
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Basic data | Standard ID | SN/T 2483-2010 (SN/T2483-2010) | | Description (Translated English) | Determination of sterigmatocystin contents in cereals for import and export. HPLC method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X11 | | Classification of International Standard | 67.060 | | Word Count Estimation | 14,167 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import content in cereals shank song streptozotocin HPLC detection method. This standard applies to import rice, barley, oats, wheat stem song streptozotocin content is detected. | SN/T 2483-2010: Determination of sterigmatocystin contents in cereals for import and export. HPLC method
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Determination of sterigmatocystin contents in cereals for import and export.HPLC method
Exit inspection and quarantine industry standard book People's Republic of China
Importers song in Grains handle cephalosporin content detection method
Liquid chromatography
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine.
The main drafters of this standard. Hu Zhong, Zhao Asia VETERINARY Wang Peng, Li Jing, Fu Xiaofang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Importers song in Grains handle cephalosporin content detection method
Liquid chromatography
1 Scope
This standard specifies the handle music content streptozotocin liquid chromatography detection methods import and export of cereal grains.
This standard applies to the import and export of rice, barley, oats, wheat stalk song cephalosporin content testing.
2 Method summary
Samples were extracted with acetonitrile handle song streptozotocin, C18 SPE column purification using liquid chromatography with UV or diode array detector
Determination, external standard.
3 Reagents and materials
Unless otherwise specified, reagents were of analytical grade, water is distilled water or equivalent purity.
3.1 Methanol. chromatographically pure.
3.2 acetonitrile. chromatography.
3.3 acetic acid. pure class distinctions.
3.4 acidified acetonitrile. HPLC grade acetonitrile, with acetic acid to adjust the pH value to 5.0.
3.5 C18 solid phase extraction column. 500mg, 3mL, or equivalent person. Before use, respectively 5mL methanol and 5mL water pre-rinse and keep cylinder
Moist.
3.6 Shank song streptozotocin standard substance (sterigmatocystin, C18H12O6, CASN O. 10048-13-2). purity of greater than or equal to 97%.
3.7 curved handles avermectin standard stock solution. accurately weighed amount standard (accurate to 0.0001g), dissolved in acetonitrile formulated at a concentration of 1.0mg/mL
Standard stock solution, kept in -18 ℃ refrigerator.
3.8 curved handles avermectin standard working solution. dilute the standard stock solution with the necessary liquidity into with 25ng/mL, 50ng/mL, 100ng/mL,
500ng/mL, 1000ng/mL of standard working solution. Stored at 4 ℃ refrigerator.
4 instruments and equipment
4.1 High performance liquid chromatograph. equipped with a diode array detector or a UV detector.
4.2 oscillator.
4.3 Centrifuge. 4000r/min.
4.4 rotary evaporator.
4.5 Solid phase extraction vacuum apparatus.
4.6 nitrogen blowing instrument.
4.7 Analytical balance.
5 Sample preparation and preservation
Take a representative sample 500g, crushed with a grinder and a circular hole through 830μm sieve, mix, divided into two parts, loaded into a clean container, dense
Seal and logo. In the sample preparation process, should prevent sample contamination or the occurrence of the change of residue content.
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