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SN/T 2247-2009: Chemicals. Test method of delayed type hypersensitivity
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| SN/T 2247-2009 | English | 149 |
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Chemicals. Test method of delayed type hypersensitivity
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SN/T 2247-2009
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Basic data | Standard ID | SN/T 2247-2009 (SN/T2247-2009) | | Description (Translated English) | Chemicals. Test method of delayed type hypersensitivity | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | A80 | | Classification of International Standard | 13.300 | | Word Count Estimation | 5,552 | | Date of Issue | 2009-02-20 | | Date of Implementation | 2009-09-01 | | Regulation (derived from) | 2009 No. 5 (total 113) Chinese industry standard filing Announcements | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the chemical principle of delayed-type hypersensitivity test, test methods and procedures. This standard applies to delayed-type hypersensitivity test chemicals. | SN/T 2247-2009: Chemicals. Test method of delayed type hypersensitivity---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Chemicals.Test method of delayed type hypersensitivity
Exit inspection and quarantine industry standard book People's Republic of China
Chemical Test Methods for delayed-type hypersensitivity
Posted 2009-02-20
2009-09-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard reference People's Republic of China Ministry of Health "health food inspection and evaluation of technical specifications" (2003 edition), which related to delayed
The technical content of the above specifications hypersensitivity (DTH) test method exactly the same, in a standard text format according to GB/T 1.1-2000
Made editorial changes.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard is drafted by. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau.
Units involved in the drafting of this standard. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Hu Yuezhen, Lining Tao, Chen Lu, Li Zhengwei, Wei Hongbing.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Chemical Test Methods for delayed-type hypersensitivity
1 Scope
This standard specifies the principles, test methods and procedures delayed type hypersensitivity test chemicals.
This standard applies to delayed-type hypersensitivity test chemicals.
2 Terms and Definitions
The following terms and definitions apply to this standard.
2.1
T cells were sensitized with antigen again contacted, causing T cell activation, release a variety of lymphokines, resulting in local tissue to monocytes
Lord inflammation. Generally 24h ~ 48h and reached the peak due to late, so called delayed-type hypersensitivity.
2.2
The reaction by the immune effector T cells, macrophages, NK cells mediated the like, such as delayed hypersensitivity, graft rejection, graft versus
Host disease.
Principle 3
Once again sensitized T cells exposed to the same antigen, the sensitized lymphocytes can cause local release a variety of lymphokines, resulting in hair
Health and monocyte infiltration of inflammation, manifested as skin redness, induration. Induration generally about 18h after antigen injection occurs, 24h ~
48h peak, then swelling and induration subside spontaneously.
Test Method 4
4.1 movable items, Selection
Healthy adult mice, commonly used strains of Kunming, LACA, BALB/C, etc., can be female and male animals, rats aged 6 weeks to
8 weeks. Experimental animal room temperature should be maintained 22 ℃ ± 3 ℃. Relative humidity of 30% to 70%, the choice of conventional feed, free access to food and water.
4.2 Animal grouping
Methods The animals were randomly divided into control group and exposure group, 10 animals in each group.
4.3 Exposure
4.3.1 exposure dose
Usually located three dose group and high dose group 1/5LD50 ~ 1/10LD50, low dose does not cause any immunotoxicity, at low and high dose
Room with dose group.
4.3.2 exposure pathways and exposure times
According to test purposes, the crowd common route of exposure, duration of exposure, acute or chronic exposure to physical and chemical properties as well as the test substance may be.
5 test procedure
5.1 Exposure 14d ~ 30d after the mice by intraperitoneal or intravenous injection 0.2mL2% sheep red blood cells (SRBC) (approximately 1 × 108 Ge SRBC) into
Immunohistochemical.
5.2 mice were immunized 4d, in its left hind paw injection 20μL20% SRBC (about 1 × 10
8 SRBC) were excited.
5.3 injection before and after injection 24h left hind paw thickness measured with a caliper, with its increased thickness expressed generally in mm, per
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