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SN/T 2094-2008: Determination of α-endosulfan and β-endosulfan residues in foodstuff for export and import.Enzyme linked immunosorbent assay method
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| SN/T 2094-2008 | English | 579 |
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Determination of α-endosulfan and β-endosulfan residues in foodstuff for export and import.Enzyme linked immunosorbent assay method
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SN/T 2094-2008
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PDF similar to SN/T 2094-2008
Basic data | Standard ID | SN/T 2094-2008 (SN/T2094-2008) | | Description (Translated English) | Determination of ��-endosulfan and ��-endosulfan residues in foodstuff for export and import.Enzyme linked immunosorbent assay method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 22,270 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export food a-endosulfan and ��-endosulfan residues of the total enzyme immunoassay method. This standard applies to cereals, vegetables, tea, tobacco, fruits, meat and meat products, shrimp endosulfan residues screening tests. Fish NA ELISA determination of colloidal gold strip with determination, see Appendix B. | SN/T 2094-2008: Determination of α-endosulfan and β-endosulfan residues in foodstuff for export and import.Enzyme linked immunosorbent assay method
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Determination of α-endosulfan and β-endosulfan residues in foodstuff for export and import.Enzyme linked immunosorbent assay method
Exit inspection and quarantine industry standard book People's Republic of China
Imported and exported food α- and β- endosulfan endosulfan residues
Detection ELISA
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A and Appendix B are normative appendices, Appendices C and D are informative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine, Tianjin University of Science and Technology.
The main drafters of this Standard. Kit Cheng, Wang Shuo, Zhang Hongwei, Zhang Yan, He Yan, Liu through the fire, Linan Qing, Zhao Weidong, Wei Yadong, Wu Yanhui.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Imported and exported food α- and β- endosulfan endosulfan residues
Detection ELISA
1 Scope
This standard specifies the method of enzyme-linked immunosorbent assay of total import and export food residues in α- and β- endosulfan endosulfan.
This standard applies to cereal grains, vegetables, tea, tobacco, fruits, meat and meat products, shrimp endosulfan residues screening tests. Fish NA
ELISA measurement using colloidal gold test strip assay, see Appendix B.
2 Determination
2.1 Method summary
Extracting samples α- and β- endosulfan endosulfan with methanol or acetone, detected by competitive ELISA.
2.2 Reagents and materials
Unless otherwise requested, all reagents were of analytical grade, double distilled water.
2.2.1 methanol.
2.2.2 acetone.
2.2.3 Potassium ferrocyanide 0.36mol/L.
2.2.4 zinc sulfate solution 1.04mol/L.
2.2.5 disodium hydrogen phosphate (Na2HPO4 · 12H2O).
2.2.6 sodium dihydrogen phosphate (NaH2PO4 · H2O).
2.2.7 sodium chloride.
2.2.8 anhydrous sodium acetate.
2.2.9 β- cyclodextrin.
2.2.10 urea hydrogen peroxide.
2.2.11 3,3 ', 5,5'-tetramethyl benzidine.
2.2.12 dimethyl sulfoxide.
2.2.13 phosphate buffer (pH7.4). See Appendix A.
2.2.14 Tween-20.
2.2.15 anhydrous sodium. 650 ℃ burning 4h, cooled in a desiccator to room temperature and stored in a sealed bottle aside.
2.2.16 0.5% fish skin glue solution (stored at 4 ℃).
2.2.17 masking agents dilution. 0.5% gum skin. phosphate buffer (1.20, by volume), and diluting at the time of use.
2.2.18 Solution I. methanol. masking agent dilutions (1.10, by volume).
2.2.19 enzyme markers. hapten and horseradish peroxidase conjugate, 4 ℃ preservation, with phosphate buffer in the use of 40-fold dilution,
HRP was not continue to use after dilution 8h.
2.2.20 washing liquid, see Appendix A.
2.2.21 substrate material solution A, see Appendix A.
2.2.22 substrate solution B, see Appendix A.
2.2.23 Stop Solution. sulfuric acid 1.25mol/L.
2.2.24 α, β- Dan mixing sulfur standards (English name. α, β-Endosulfan, CAS Code. 115-29-7). not less than 99.5% purity.
2.2.25 Standard stock solution. formulated as 1μg/mL stock solution in methanol, -20 ℃ preservation.
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