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SN/T 2102.1-2008: Polymerase chain reaction(PCR)for the detection of food-borne pathogens—Part 1: General requirements and definitions
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Polymerase chain reaction(PCR)for the detection of food-borne pathogens—Part 1: General requirements and definitions
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SN/T 2102.1-2008
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Basic data | Standard_ID | SN/T 2102.1-2008 (SN/T2102.1-2008) | | Description (Translated English) | Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Part 1: General requirements and definitions | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 29,274 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Quoted Standard | GB/T 27025; SN/T 2102.3; SN/T 2102.4; WS/T 230 | | Adopted Standard | ISO 22174-2005, IDT | | Drafting Organization | The People's Republic of China Entry-Exit Inspection and Quarantine Bureau in Shanxi | | Administrative Organization | National Certification and Accreditation Administration Committee | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Proposing organization | National Certification and Accreditation Administration Standardization Committee | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China | | Summary | This standard specifies the in vitro nucleic acid sequences (DNA or RNA) amplification General requirements. Provides the detection of food and food isolates of pathogens by PCR method. This standard applies to pathogen detection in food and feed, also applies to other samples (such as environmental samples) and non-pathogenic microorganisms is detected. | SN/T 2102.1-2008: Polymerase chain reaction(PCR)for the detection of food-borne pathogens—Part 1: General requirements and definitions
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Polymerase chain reaction (PCR) for the detection of food-borne pathogens-Part 1. General requirements and definitions
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
SN/T 2102.1-2008/ISO 22174.2005
Technical specification for PCR detection of foodborne pathogens
Part 1. General requirements and definitions
[ISO 22174.2005, Microbiologyoffoodandanimalfeedingstuffs-
Generalrequirementsanddefinitions, IDT]
Released on.2008-07-17
2009-02-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 2102 "Technical Specifications for PCR Detection of Foodborne Pathogens" is divided into four parts.
--- Part 1. General requirements and definitions;
--- Part 2. PCR test performance test requirements;
--- Part 3. Qualitative testing methods for sample preparation requirements;
--- Part 4. Qualitative detection methods for amplification and testing requirements.
This part is the first part of SN/T 2102.
This part is equivalent to ISO 22174.2005 "Food and animal feed microbiology food-borne pathogens PCR detection common
Summation definition, and made the following editorial changes.
--- Modified the "Foreword" and "Introduction";
--- The description of the "normative reference document" was modified in accordance with the requirements of GB/T 1.1;
--- Change some of the "notes" in the standard to the body according to the Chinese standard;
--- Replace the international standards in the standard with the corresponding national standards or industry standards, such as. GB/T 27025 instead of ISO 17025;
--- Change "Warning" to "Security Requirements and place it at the end of the standard".
This part is proposed and managed by the National Certification and Accreditation Administration.
This part is from the Shanxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Chinese people
Inner Mongolia Entry-Exit Inspection and Quarantine Bureau, Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, and entry and exit of Liaoning Province of the People's Republic of China
The Inspection and Quarantine Bureau is responsible for drafting.
The main drafters of this section. Li Weihua, Li Xiaohong, Liu Middle School, Jiang Yuan, Cao Jijuan, Wang Haiyan.
This section is the first industry standard for entry-exit inspection and quarantine.
SN/T 2102.1-2008/ISO 22174.2005
Introduction
Polymerase chain reaction (PCR) has the advantages of rapidity, sensitivity and specificity. PCR is used to detect foodborne microorganisms.
The law has developed very rapidly in recent years.
PCR technology can be divided into DNA amplification (reverse transcription PCR) and RNA amplification (PCR) depending on the type of target nucleic acid to be amplified.
Based on the above two types of PCR technologies, a variety of PCR detection techniques have been developed and derived.
ISO 22174.2005, by the European Committee for Standardization (CEN) Food Analysis - Level Method Technical Committee (CEN/T C275) and
The Food Products Technical Committee (ISO /T C34) Microbiological Subcommittee (SC9) is jointly developed in accordance with the ISO and CEN Technical Cooperation Agreement.
This section is equivalent to ISO 22174.2005, which is the first part of the "Technical Specifications for PCR Detection of Foodborne Pathogens". Food source
General requirements for in vitro PCR amplification and detection of sexual pathogens and to ensure comparability and reproducibility of test results from different laboratories.
SN/T 2102.1-2008/ISO 22174.2005
Technical specification for PCR detection of foodborne pathogens
Part 1. General requirements and definitions
1 Scope
This part of SN/T 2102 specifies the general requirements for in vitro nucleic acid sequence (DNA or RNA) amplification. Provision for testing food and food
PCR method for pathogens in product isolates.
This section applies to the detection of pathogens in food and feed, as well as to other samples (such as environmental samples) and non-pathogenic microorganisms.
2 Normative references
The terms in the following documents become the provisions of this part by reference to this part of SN/T 2102. For dated references,
All subsequent amendments (not including errata content) or revisions do not apply to this section, however, an agreement is reached under this section.
The parties studied whether the latest version of these documents could be used. For undated references, the latest edition applies to this section.
General requirements for GB/T 27025 testing and calibration laboratory capabilities
Detecting of food-borne pathogens - Part 3. Qualitative test methods
SN/T 2102.4 Technical specification for detection of foodborne pathogens - Part 4. Qualitative method
Application of Polymerase Chain Reaction (PCR) Technology in WS/T 230 Clinical Diagnosis
3 Terms and definitions
The following terms and definitions apply to this part of the SN/T 2102.
3.1 General terminology
3.1.1
A biological macromolecule that acts as a genetic information medium or an information expression factor. Nucleic acids are available in both DNA and RNA types.
3.1.2
A deoxyribonucleotide polymer in the form of a double strand or a single strand.
3.1.3
A ribonucleotide polymer in the form of a double strand or a single strand.
3.1.4
Samples for analysis that may differ in chemical composition or physical state.
3.1.5
The same operator used the same instrument and method in the same laboratory to obtain independent results in shorter time intervals.
3.1.6
Different operators use different instruments and the same method in different laboratories to test the conditions of the same project to obtain test results.
SN/T 2102.1-2008/ISO 22174.2005
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