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SN/T 2102.2-2008: Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Part 2: Performance testing for thermal cyclers
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 2102.2-2008 | English | 554 |
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Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Part 2: Performance testing for thermal cyclers
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SN/T 2102.2-2008
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Basic data | Standard ID | SN/T 2102.2-2008 (SN/T2102.2-2008) | | Description (Translated English) | Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Part 2: Performance testing for thermal cyclers | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Word Count Estimation | 29,263 | | Date of Issue | 2008-07-17 | | Date of Implementation | 2009-02-01 | | Quoted Standard | GB/T 27025; SN/T 2102.1 | | Adopted Standard | ISO/TS 20836-2005, IDT | | Regulation (derived from) | Industry standard filing Notice 2008 No. 9 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the PCR instrument installation, use and maintenance of the basic requirements of the provisions of the PCR instrument performance test biochemical and physical methods. PCR instrument cooling/heating element has a specified life of its normal function of the play depends not only on the quality of design, but also on the proper use and maintenance. This standard applies to PCR instrument biochemical and physical performance tests. | SN/T 2102.2-2008: Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Part 2: Performance testing for thermal cyclers
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Polymerase chain reaction (PCR) for the detection of food-borne pathogens.Part 2. Performance testing for thermal cyclers
Exit inspection and quarantine industry standard book People's Republic of China
SN/T 2102.2-2008/ISO /TS20836.2005
PCR detection of foodborne pathogens Technical Specifications
Part 2. PCR instrument performance test requirements
[ISO /T S20836.2005, Microbiologyoffoodandanimalfeedingstuffs-
Polymerasechainreaction (PCR) forthedetectionoffood-bornepathogens-
Performancetestingforthermalcyclers, IDT]
Posted 2008-07-17
2009-02-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2102. "Foodborne pathogens PCR detection technology specifications" is divided into four parts.
--- Part 1. General requirements and definitions;
Part --- Article 2. PCR instrument performance test requirements;
--- Part 3. Qualitative detection methods for sample preparation requirements;
--- Part 4. Qualitative PCR amplification detection requirements.
This section SN/T 2102 Part 2.
This part identical with ISO /T S20836.2005 "Microbiology of food and animal feed to detect foodborne pathogens PCR PCR
Instrument performance test requirements, "and made the following editorial changes.
--- On the "Preface" and "Introduction" has been modified;
--- On the "Normative references" description according to GB/T 1.1 requirements were modified;
--- The standard international standards with the corresponding national standards or industry standard alternative, such as. GB/T 27025 instead of ISO 17025;
--- The first A. Chapter 3 reagent concentration of expression to Chinese way of expression;
--- The "warning" to "security requirements, and placed into a standard end of the paragraph;
--- Deleted after the reference standard.
This section of the Appendices A and B are informative appendices.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This part of the Chinese Academy of Inspection and Quarantine, Shanxi People's Republic of China Exit Inspection and Quarantine, People's Republic of China Shanghai
Entry-Exit Inspection and Quarantine of Jilin People's Republic of China, People's Republic of China Guangdong Entry Inspection and Quarantine Bureau,
People's Republic of China Shandong CIQ is responsible for drafting.
The main drafters of this section. Li Weihua, Zhaogui Ming, Li Xiaohong, Wang Zhenguo, Xu Longyan, Ray quality text, Zouming Jiang, Liu Shouxian.
The first part of the Department of Inspection and Quarantine issued by industry standards.
SN/T 2102.2-2008/ISO /TS20836.2005
Introduction
Polymerase chain reaction (PCR) is a rapid, sensitive and specific advantages of the use of PCR technology for the detection of foodborne microbes party
Method development in recent years very rapidly.
Currently, PCR instrument in the technical aspects have been perfect, but due to the impact of Perot Peltier effect and the production process, heating, cooling components with
There is a certain life, the normal function of these components depends on their quality and proper use and maintenance. For this reason, be
PCR instrument routine maintenance is still very necessary.
ISO /T S20836.2005, by the European Committee for Standardization (CEN) Technical Committee on Food Analysis level (CEN/T C275)
Microbial Subcommittee (SC9) in accordance with ISO and CEN technical cooperation agreement with the United Food Products Technical Committee (ISO /T C34)
Making.
This part identical with ISO /T S20836.2005, as "PCR detection of foodborne pathogens technical specifications" Part 2. It provides
PCR instrument (including cooling/heating element) installation, use and maintenance of general principles. Information Appendix A and Appendix B provides PCR instrument
Biochemical and physical performance test method.
SN/T 2102.2-2008/ISO /TS20836.2005
PCR detection of foodborne pathogens Technical Specifications
Part 2. PCR instrument performance test requirements
1 Scope
This section SN/T 2102 established the PCR instrument installation, use and maintenance of the basic requirements, the provisions of the PCR Testing the Performance of Health
And physical methods. Cooling/heating element PCR instrument has a specified life of its normal functioning depends not only on design
Quality also depends on proper use and maintenance.
This section applies to the biochemical and physical performance test PCR instrument.
2 Normative references
The following documents contain provisions which, through reference SN/T 2102 to the present, constitute provisions of this section. For dated reference documents
Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section
Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this
section.
GB/T 27025 testing and calibration laboratories General requirements
SN/T 2102.1 foodborne pathogens PCR Detection Technology Specification - Part 1. General requirements and definitions
3 Terms and Definitions
SN/T 2102.1 and established the following terms and definitions apply to SN/T 2102 to the present section.
3.1
The reaction tube, the reaction solution was evaporated means preventing PCR instrument.
3.2
Temperature control device (such as a heating module) uniformity of temperature.
3.3
(Such as temperature-sensitive PCR reactions) Test procedures PCR instrument performance by biochemical methods.
3.4
PCR instrument performance test procedures determined by physical methods.
3.5
The PCR result deviates from a predetermined temperature or chemical control program which led to the PCR amplification of the effect of weakening the system.
3.6
PCR instrument porous plate in the actual temperature and the temperature of the sample is displayed or regions prone position deviation.
4 PCR instrument installation
According to the manufacturer specification requirements should be installed at a suitable temperature (5 ℃ ~ 40 ℃) and humidity (RH ≤80%) Environment
SN/T 2102.2-2008/ISO /TS20836.2005
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