| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1699.2-2006 | English | 239 |
Add to Cart
|
3 days [Need to translate]
|
Protocol of direct immunofluorescent test for porcine epidemic diarrhea
| Obsolete |
SN/T 1699.2-2006
|
PDF similar to SN/T 1699.2-2006
Basic data | Standard ID | SN/T 1699.2-2006 (SN/T1699.2-2006) | | Description (Translated English) | Protocol of direct immunofluorescent test for porcine epidemic diarrhea | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 6,624 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-16 | | Quoted Standard | GB/T 6822 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the porcine epidemic diarrhea virus direct immunofluorescence test method of operation. This standard applies to the diagnosis of porcine epidemic diarrhea. | SN/T 1699.2-2006: Protocol of direct immunofluorescent test for porcine epidemic diarrhea---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of direct immunofluorescent test for porcine epidemic diarrhea
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Porcine epidemic diarrhea virus direct immunization
Fluorescence test procedure
Released on.2006-01-26
Implementation of.2006-08-16
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 1699 is divided into three parts.
--- Swine epidemic diarrhea trace serum neutralization test procedures;
--- Swine epidemic diarrhea virus direct immunofluorescence test procedures;
--- Indirect spot enzyme-linked immunosorbent assay protocol for porcine epidemic diarrhea.
This part is the second part of SN/T 1699.
Appendix A of this section is a normative appendix.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Luo Qiong, Liang Gang, Lin Zhixiong, Li Lishi.
This section is the first industry standard for entry-exit inspection and quarantine.
Direct immunofluorescence test protocol for porcine epidemic diarrhea virus
1 Scope
This part of SN/T 1699 specifies the method of direct immunofluorescence testing of porcine epidemic diarrhea virus.
This section applies to the diagnosis of porcine epidemic diarrhea.
2 Normative references
The provisions in the following documents become the provisions of this part by reference to this part of SN/T 1699. Quotations with dated
, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section
The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this
section.
GB/T 6822 Analytical laboratory water specifications and test usage
3 Abbreviations
The following abbreviations apply to this part of SN/T 1699.
3.1
PED
Pig epidemic diarrhea.
4 instruments
Fluorescence microscopy, cryostat, ophthalmic tweezers, slides, coverslips, staining cylinders.
5 test materials
See A. for the preparation method. 1.1, or according to the diagnostic reagent instructions.
5.2 Glycerol buffered saline
See A. for the preparation method. 1.2, or according to the diagnostic reagent instructions.
5.3 0.2‰ Evans Blue Solution
See A. for the preparation method. 1.3, or according to the diagnostic reagent instructions.
5.4 Fluorescent antibodies
A rabbit anti-PED virus antibody that incorporates fluorescein isothiocyanate (FITC). Provided by the designated unit, saved and used according to the instructions.
5.5 water
The water used in this section meets the specifications of the secondary water in GB/T 6822.
5.6 Samples to be inspected
Take a jejunum of about 10 cm long dead pigs. The samples should be taken aseptically and without spoilage.
5.7 positive samples
Specified by the unit.
5.8 negative sample
Specified by the unit.
|