SN/T 1681: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1681-2023 | English | RFQ |
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3 days [Need to translate]
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(Technical specifications for quarantine of honey bee larval putrid disease)
| Valid |
SN/T 1681-2023
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| SN/T 1681-2011 | English | 479 |
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3 days [Need to translate]
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Quarantine protocol for American foulbrood of honey bees
| Obsolete |
SN/T 1681-2011
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| SN/T 1681-2005 | English | 279 |
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3 days [Need to translate]
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The bee the Americas larvae rancid disease diagnosis method
| Obsolete |
SN/T 1681-2005
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PDF similar to SN/T 1681-2023
Basic data | Standard ID | SN/T 1681-2023 (SN/T1681-2023) | | Description (Translated English) | (Technical specifications for quarantine of honey bee larval putrid disease) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Date of Issue | 2023-11-01 | | Date of Implementation | 2024-05-01 | | Older Standard (superseded by this standard) | SN/T 1681-2011 | | Issuing agency(ies) | General Administration of Customs | SN/T 1681-2011: Quarantine protocol for American foulbrood of honey bees---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for American foulbrood of honey bees
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1681-2005
American bee larvae rancid disease quarantine Technical Specifications
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard replaces the SN/T 1681-2005 "bee larvae rancid American disease diagnosis."
This standard compared with SN/T 1681-2005, the main technical changes are as follows.
--- Changing the name of the standard;
--- Increased "site inspection and quarantine," "physiological characterization" and "PCR Detection" content;
--- Modification of the original standard "sampling", "direct test" and "Isolation and identification."
This revised standard adopts Office International des Epizooties (OIE) "Terrestrial animal disease diagnostic tests and vaccines Standards Manual" (2008 edition) 2.2.2
Chapter, the main change is as follows.
--- Description of "introduction" and so, as amended, abbreviated as the present standard in Appendix A;
--- In its original format and content have been adjusted on the order of typesetting.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Hubei Exit Inspection and Quarantine.
The main drafters of this standard. Guo STARS Zengxian Dong, Pinghan Li, Chen Jianjun, Zhao Hui, Yan Zhili, Zhu Tong Ming Wang Zhenhua.
This standard replaces the standards previously issued as follows.
--- SN/T 1681-2005.
American bee larvae rancid disease quarantine Technical Specifications
1 Scope
This standard specifies the Americas bee larvae rancid disease site inspection and quarantine, sampling, laboratory diagnosis and quarantine technical specifications.
This standard applies to the entry and exit of American bee larvae rancid disease quarantine and epidemiology, diagnosis and monitoring of the disease.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 Reagents and materials
3.1 Bacillus larvae (Paenibacilluslarvaesubsp.larvae)
Standard strains designated by the OIE Reference Laboratory or the State Administration of Quality Supervision, Inspection and Quarantine laboratory.
3.2 Primer
3.2.1 PCR primers
Upstream primer. 5'-CTTGTGTTTCTTTCGGGAGACGCC-3 '
Downstream primer. 5'-TCTTAGAGTGCCCACCTCTGCG-3 '
Amplified fragment size. 1106bp.
3.2.2 Nested PCR primers
3.2.2.1 outer nested PCR primers
Upstream primer. 5'-TCGAGCGGACCTTGTGTT-3 '
Downstream primer. 5'-CTATCTCAAAACCGGTCAGAG-3 '
Amplified fragment size. 969bp.
3.2.2.2 medial nested PCR primers
Upstream primer. 5'-CTTCGCATGAAGTCATG-3 '
Downstream primer. 5'-TCAGTTATAGGCCAGAAAGC-3 '
Amplified fragment size. 572bp.
3.3 Taq enzyme.
3.4 dNTPs. containing dCTP, dGTP, dATP, dTTP each 10mmol/L.
3.5 DNAMarker.
3.6 ethidium bromide.
3.7 isopropanol.
3.8 10mmol/LTris ยท HCl-1mmol/LEDTA solution (pH8.0).
3.9 carrot yeast agar medium.
3.10 Potato media.
3.11 yeast extract media.
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