SN/T 1682: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1682-2020 | English | RFQ |
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3 days [Need to translate]
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(Technical specification for quarantine of European larval foul disease of honeybee)
| Valid |
SN/T 1682-2020
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| SN/T 1682-2010 | English | 439 |
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3 days [Need to translate]
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Quarantine protocol for European foulbrood of honey bees
| Obsolete |
SN/T 1682-2010
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| SN/T 1682-2005 | English | 239 |
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2 days [Need to translate]
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Protocol of diagnosis for European foulbrood of honey bees
| Obsolete |
SN/T 1682-2005
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PDF similar to SN/T 1682-2020
Basic data | Standard ID | SN/T 1682-2020 (SN/T1682-2020) | | Description (Translated English) | (Technical specification for quarantine of European larval foul disease of honeybee) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 07.080 | | Date of Issue | 2020-12-30 | | Date of Implementation | 2021-07-01 | | Older Standard (superseded by this standard) | SN/T 1682-2010 | | Regulation (derived from) | General Administration of Customs Announcement No. 136 [2020] | | Issuing agency(ies) | General Administration of Customs | SN/T 1682-2010: Quarantine protocol for European foulbrood of honey bees---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for European foulbrood of honey bees
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1682-2005
European bee larvae rancid disease quarantine Technical Specifications
Issued on. 2010-11-01
2011-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1682-2005 "European bee larvae rancid disease diagnosis."
This revised standard adopts the OIE "terrestrial animal diagnostic tests and vaccines Handbook" (2010 edition) in section 2.2.3.B diagnostic techniques
Content, covering microscopy, culture methods and polymerase chain reaction.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Jilin People's Republic of China Exit Inspection and Quarantine, Beijing People's Republic of China Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Wang Zhenguo, Chenshi Song, Liu Jinhua, Song Yun war, Cai Yang, Su Lin, Meng Qingfeng, Zhu Kaiyuan, Wang Jian, WEI Chunyan,
Spacing Form.
This standard replaces the standards previously issued as follows.
--- SN/T 1682-2005.
European bee larvae rancid disease quarantine Technical Specifications
1 Scope
This standard specifies the method for the diagnosis of European bee larvae rancid disease.
This standard applies to the European honeybee larvae rancid diagnosis.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 18088 Entry and Exit Animal Quarantine sampling
3 diagnostic principles
European bee larvae rancid disease (Europeanfoulbroodofhoneybees) is a hive of bees cocci (Melissococcuspluto-
nius) caused the death of bee larvae a bacterial sexually transmitted diseases, the rapid growth of the colony when multiple, the most common symptoms are obvious bee larvae
In the nest room closure shortly before death. Most of the larvae died of illness at the bottom of the nest room curled deformed larvae will soon be many sick care workers
Bee found, remove, leaving the house empty nest. Worker bees are not infected larvae died shortly cleared softened by pale yellow becoming brown, melted into half
Liquid mass, then dry, forming a dark flake easily removed from the nest room. There is a serious infection of the larvae sour smell. Cause honey
Death bee larvae there are other diseases and causes of the field based on clinical symptoms and therefore can not make a reliable diagnosis.
When suspected based on clinical symptoms of the disease in Europe when the larvae rancid, it is desirable to illness or death larvae smear staining, make a preliminary diagnosis
Off. Under ordinary optical microscope, bee hive bacteria were single or a series or end to end in pairs or short chains arranged short lanceolate ball
Bacteria, size 0.5μm × 1.0μm. The exact diagnosis requires bacterial isolation and culture and staining identification, or semi-nested polymerase
Chain reaction method (polymerasechainreaction, PCR) of isolated cultures were identified. Also can be directly used semi-nested PCR
Illness or death of the larvae to be confirmed.
This standard is used in semi-nested PCR, a pair of primers was designed according to the bee hive bacteria 16SrRNA gene (MP1 and
The MP2), for the first cycle of PCR amplification, the amplified fragment is 486bp (see Appendix A). The amplified gene as a template to design primers Article
(MP3), and MP1 to MP3 as second PCR primers, amplification fragments of 276bp.
4 equipment, materials and reagents
4.1 Device
Electrophoresis, gel imaging analysis system, high-speed desktop refrigerated centrifuge (maximum speed 12000r/min or more), PCR amplification, general
Through an optical microscope, CO2 incubator and the like.
4.2 Materials and Reagents
The method of reagent preparation see Appendix B, the water is a water according to GB/T 6682 regulations. The reagents used primarily for DNA extraction
Buffer, of CTAB precipitating solution, 1.2mol L sodium chloride solution/chloroform, isopropanol, 70% ethanol, TaqDNA polymerase, 10 ×
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