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Protocol of complement fixation test for african horse sickness
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SN/T 1692.3-2006
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Basic data | Standard ID | SN/T 1692.3-2006 (SN/T1692.3-2006) | | Description (Translated English) | Protocol of complement fixation test for african horse sickness | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 9,943 | | Date of Issue | 2006-04-25 | | Date of Implementation | 2006-11-15 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 7 (No. 79 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the African horse sickness complement fixation test procedures. This standard applies to the inspection and quarantine of African horse sickness. | SN/T 1692.3-2006: Protocol of complement fixation test for african horse sickness---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of complement fixation test for african horse sickness
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
African horse stable complement test procedure
Released on.2006-04-25
Implementation of.2006-11-15
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
SN/T 1692 is divided into three parts.
--- African horse stable agar immunodiffusion test operating procedures;
--- African horse stable hemagglutination and hemagglutination inhibition test procedures;
--- African horse stable complement test procedures.
This part is the third part of SN/T 1692.
Appendix A of this section is a normative appendix.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Dong Jun, Zhou Xiaoli, Hua Qunyi, Xiao Ronghai, Yang Yunqing, Ying Xuesong.
This section is the first industry standard for entry-exit inspection and quarantine.
African horse stable complement test procedure
1 Scope
This part of SN/T 1692 specifies the procedure for the African Stirrup Complement Binding Test.
This section applies to the inspection and quarantine of African horse stables.
2 Normative references
The terms in the following documents become the provisions of this part by reference to this part of SN/T 1692. For dated references,
All subsequent amendments (not including errata content) or revisions do not apply to this section, however, an agreement is reached under this section.
The parties studied whether the latest version of these documents could be used. For undated references, the latest edition applies to this section.
GB/T 6682 Analytical laboratory water specifications and test methods
3 reagents and materials
The water used in this section should meet the specifications of the third grade water in GB/T 6682. The reagents used are analytically pure reagents unless otherwise specified.
3.1 African Stirrup Anti-Antigen. Provided by the designated unit or prepared according to Appendix A.
3.2 Complement. Provided by the designated unit or prepared in accordance with Appendix A.
3.3 Hemolysin. provided by the designated unit.
3.4 Positive serum. provided by the designated unit, diluted 1.2 before use, and extinguished in a water bath at 58 °C ~ 59 °C for 30 min.
3.5 Negative serum. provided by the designated unit, diluted 1.10 before use, and extinguished at 58 ° C ~ 59 ° C water bath for 30 min.
3.6 Alzheimer's solution. Prepared according to Appendix A.
3.7 Barbiturate Buffer (VBS). Prepared according to Appendix A.
4 instruments and equipment
4.1 Each type of dripper is 1.034×105Pa high pressure for 15min, and dried for use.
4.2 Freezer high speed centrifuge.
4.3 Water bath.
4.4 Micropipettes.
5 sample processing
After the animal number to be inspected is registered, the blood is collected aseptically, centrifuged at 5000 r/min 10 ° C for 10 min to 15 min, and the serum is separated aseptically.
Store at -70 ° C (for a formal test, one spare), use 1.100 dilution with barbital buffer before use, horse serum 56 ° C, zebra blood
Clear 60 ° C, sputum serum 62 ° C water bath for 30 min inactivated.
6 preliminary test
6.1 2% sheep red blood cell suspension preparation
6.1.1 Take one sterilized flask containing 100 mL of albino solution, and collect 50 mL of sterile blood from the jugular vein of the ram. After mixing, set the refrigerator at 4 °C.
Set 4d ~ 5d.
6.1.2 When inhalation, inhale the blood into the centrifuge tube, centrifuge at 1500r/min for 15min, and use a pipette to remove the supernatant and white blood cells from the upper layer of red blood cells.
The platelet membrane was washed with BBS buffer, centrifuged at 1500 r/min for 15 min, and the supernatant was discarded, and the washing was repeated four times.
6.1.3 Dilute the last washed red blood cell sludge with VBS buffer at 2% (2mL cells + 98mL VBS)
2% suspension).
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