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Protocol of agar-gel immunodiffusion test for African horse sickness
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SN/T 1692.1-2006
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Basic data | Standard ID | SN/T 1692.1-2006 (SN/T1692.1-2006) | | Description (Translated English) | Protocol of agar-gel immunodiffusion test for African horse sickness | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 5,586 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the African horse sickness agar immunodiffusion test procedures. This standard applies to African horse sickness AGP antibodies. | SN/T 1692.1-2006: Protocol of agar-gel immunodiffusion test for African horse sickness---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of agar-gel immunodiffusion test for African horse sickness
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
African horse stable agar immunodiffusion test operation procedure
Released on.2006-01-26
Implementation of.2006-08-16
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This section is based on the World Organisation for Animal Health (OIE) Handbook of Diagnosis and Vaccines for Terrestrial Animal Diseases.
SN/T 1692 is divided into three parts.
--- African horse stable agar immunodiffusion test operating procedures;
--- African horse stable hemagglutination and hemagglutination inhibition test procedures;
--- African horse stable complement test procedures.
This part is the first part of SN/T 1692.
This part is proposed and managed by the National Certification and Accreditation Administration.
This section drafted by. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this section. Yang Jingyan, Xu Zizhong, Chen Jianjun, Long Zhongbao.
This section is the first industry standard for entry-exit inspection and quarantine.
African horse stable agar immunodiffusion test operation procedure
1 Scope
This part of SN/T 1692 specifies the protocol for the African equine agar immunodiffusion test.
This section applies to the detection of African horseshoe agaric expansion antibodies.
2 Normative references
The terms in the following documents become the provisions of this part by reference to this part of SN/T 1692. Quotations with dated
, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouragement is achieved under this section
The parties to the agreement study whether the latest versions of these documents can be used. For undated references, the latest edition applies to this
section.
GB/T 6682 Analytical laboratory water specifications and test methods
3 reagents and materials
3.1 Antigen. African horse stable tissue culture antigen.
3.2 African horse stable positive serum.
3.3 plate.
3.4 Micropipettes.
3.5 micro dripper.
3.6 Erlenmeyer flask.
3.7 agarose.
3.8 Sodium chloride. analytically pure.
3.9 Sodium azide. analytically pure.
3.10 Water. Meet the secondary water specifications of the GB/T 6682 analytical laboratory.
4 steps
4.1 Preparation of agar gel plate
Weigh 1.2g of agarose, 1.0g of sodium chloride, add 100mL of water, dissolve it by high pressure or boiling after 68.94kPa, 10min, then cool to
At 45 ° C, 0.01 g of sodium azide was added. Add agar to each plate and make the agar plate thickness 2.5mm~2.8mm, and then invert after solidification.
Put in a refrigerator at 4 ° C for 15 min.
4.2 punching
1 hole in the middle and 6 holes in the surrounding group. The aperture is 4 mm and the hole spacing is 2.4 mm. Use a needle to pick out or aspirate the agar in the well.
4.3 Loading
The central well was added with antigen, 1, 3, and 5 wells plus standard positive serum; 2, 4, and 6 wells were added to the test serum. The amount of sample is based on the hole level, and the sample is placed after the wet box.
The reaction was carried out at room temperature (22 ° C to 25 ° C), and a positive control was set for each test.
5 judgment
5.1 24h initial judgment, 72h final judgment. When the positive control is established, a determination is made.
Positive. a dense sediment line appears between the test serum well and the antigen well, and is smooth with the end of the sedimentation line of the standard positive serum.
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