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Determination of diazepam in muscle of livestock and poultry. High performance liquid chromatography
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SB/T 10501-2008
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Basic data | Standard ID | SB/T 10501-2008 (SB/T10501-2008) | | Description (Translated English) | Determination of diazepam in muscle of livestock and poultry. High performance liquid chromatography | | Sector / Industry | Domestic Trade Industry Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 7,783 | | Date of Issue | 2008-09-27 | | Date of Implementation | 2009-03-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | ?Ministry of Commerce Announcement 2008 No.72 | | Issuing agency(ies) | Ministry of Commerce of the People's Republic of China | | Summary | This standard provides high-performance liquid diazepam chromatography (HPLC) method for determination of poultry meat. This standard applies to chicken and pork diazepam residue testing. The detection limit of this method is 0. 01mg/kg. | SB/T 10501-2008: Determination of diazepam in muscle of livestock and poultry. High performance liquid chromatography
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Determination of diazepam in muscle of livestock and poultry.High performance liquid chromatography
ICS 67.050
X04
Record number. 25147-2008
People's Republic of China Commercial Industry Standard
Determination of diazepam in livestock and poultry
High performance liquid chromatography
Released on.2008-09-27
2009-03-01 implementation
Published by the Ministry of Commerce
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the Ministry of Commerce of the People's Republic of China.
This standard was drafted by the Slaughter Technical Appraisal Center of the Ministry of Commerce of the People's Republic
The main drafters of this standard. Hou Xiaolin, Song Dehua, Jin Shesheng, Liu Wenjuan.
This standard is entrusted to the Ministry of Commerce's Slaughter Technology Appraisal Center for interpretation.
Determination of diazepam in livestock and poultry
High performance liquid chromatography
1 Scope
This standard specifies high performance liquid chromatography (HPLC) methods for the determination of diazepam in livestock and poultry meat.
This standard applies to the detection of diazepam residues in chicken and pork.
The minimum detection limit of this method is 0.01 mg/kg.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods (GB/T 6682-2008, ISO 3639.1987, MOD)
3 Principle of the method
The diazepam in the sampled tissue was extracted with acetonitrile, purified by solid phase extraction, and detected by reversed-phase high performance liquid chromatography-ultraviolet detector.
Quantify by external standard method.
4 reagents and materials
Unless otherwise specified, the reagents involved in the method are of analytical grade, and the water is secondary water in accordance with GB/T 6682.
4.1 Acetonitrile. chromatographically pure.
4.2 Methanol. chromatographically pure.
4.3 Ammonium acetate.
4.4 Isopropanol.
4.5 Acetic acid.
4.6 Perchloric acid.
4.7 Triethylamine.
4.8 Diazepam standard.
4.9 C18 solid phase extraction cartridge. 500 mg, 3 mL, or equivalent.
4.10 1% perchloric acid solution. Take 1 mL of perchloric acid and dilute to 100 mL with water.
4.11 Microporous syringe filter 0.2μm.
4.12 standard solution
4.12.1 Standard stock solution
Weigh accurately 50.0mg (converted to pure substance according to purity) diazepam standard, dissolved in acetonitrile and set in 50mL volumetric flask
Capacitance, stable at -18 °C for 6 months.
4.12.2 Standard working fluid
Before use, take the above standard stock solution and dilute it with a 1% aqueous solution of perchloric acid to a concentration of 0.05 μg/mL, 0.10 μg/mL.
A standard working solution of 0.20 μg/mL, 0.50 μg/mL, and 1.00 μg/mL was injected for measurement.
4.13 Eluent. methanol. water. triethylamine (35. 65. 0.1, by volume).
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