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LY/T 1881-2010: Embryo excision test for woody plants
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Embryo excision test for woody plants
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LY/T 1881-2010
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Basic data | Standard ID | LY/T 1881-2010 (LY/T1881-2010) | | Description (Translated English) | Embryo excision test for woody plants | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B61 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 12,150 | | Date of Issue | 2010-02-09 | | Date of Implementation | 2010-06-01 | | Quoted Standard | GB 2772-1999; GB 7908 | | Regulation (derived from) | State Forestry Administration Notice 2010 No. 2; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | State Forestry Administration | | Summary | This standard specifies listed in Appendix A, Table Al of 61 woody seed viability was measured in vitro embryo technology. This standard applies to woody seed producers, operators and users of the seed viability test. | LY/T 1881-2010: Embryo excision test for woody plants---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Embryo excision test for woody plants
ICS 65.020.01
B61
People's Republic of China Forestry Industry Standard
Isolation technique of woody plant seeds in vitro
Released on.2010-02-09
2010-06-01 implementation
State Forestry Administration released
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Forest Seed Standardization Technical Committee.
This standard was drafted. Forestry Research Institute of Chinese Academy of Forestry.
Participated in the drafting of this standard. State Forestry Bureau and Seedling Work Station of the State Forestry Administration, Nanjing Forestry University, Yunnan Province, forestry seedlings work
The terminus, the forest seedling station in Chifeng City, Inner Mongolia.
The main drafters of this standard. Li Qingmei, Zheng Xinmin, Zhang Hongyan, Tian Shuxia, Li Jiping, Yu Fangyuan, Shen Yongbao, Yan Xiuli.
Isolation technique of woody plant seeds in vitro
1 Scope
This standard specifies Appendix A, Table A. The technique for measuring the viability of 61 woody plant seeds isolated from 1 in vitro.
This standard applies to the test of seed viability of woody plant seed producers, operators and users.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard. However, parties to agreements based on this standard are encouraged to
Whether the latest version of these files can be used. For undated references, the latest edition applies to this standard.
GB 2772-1999 Forest seed inspection procedures
GB 7908 forest tree seed quality classification
3 Terms and definitions
The following terms and definitions apply to this standard.
3.1
Techniques and methods for in vitro culture of embryos of plants by aseptic manipulation under artificially controlled environmental conditions.
3.2
The potential for germination of seeds and the vitality of embryos usually refers to the number of viable (ie, living) seeds in a batch of seeds.
The percentage of the total number of seeds.
4 procedures
4.1 Determination of samples
The assay requires 400 seeds of pure seed, 4 replicates, and 100 replicates per replicate. Sampling should be at least from the pure seeds after the clarity analysis
425 to 450 seeds were obtained so that there were enough seeds to compensate for the embryos that might be damaged during the exfoliation process.
4.2 Pretreatment
4.2.1 Remove hard skin or seed coat by mechanical or chemical means.
4.2.2 According to Appendix A, Table A. Soak the seeds in the conditions specified in 1 and change the water once every 24 hours. After soaking, disinfect with 30% hydrogen peroxide for 30min
Or disinfect with 2% sodium hypochlorite for 10 min, then rinse twice with water.
4.3 Embryo stripping
The test equipment and work surface should be disinfected. Exfoliate the embryo from the swollen seed with a scalpel or blade and place it in a culture vessel.
Wet filter paper, gauze or cotton wool should be kept moist throughout the operation. Note that the embryo should not be damaged during the stripping process and damaged during separation.
The injured embryo should be discarded and supplemented with excess seeds from the assay sample (4.1).
The empty granules, non-embryonic granules, rotted granules and pest worms found at the time of peeling were counted as non-viable seeds.
4.4 Training
According to Appendix A, Table A. 1 specified culture conditions and culture time to culture the embryos, observe daily, and use embryos for secondary infections.
After the water is washed, it is returned to the vessel and the decayed embryos are sorted out.
If the test sample is severely infected by mold, a retest is required.
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