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GB/T 39917-2021 English PDF

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GB/T 39917-2021: Variety genuineness and purity testing of main crops with SSR markers - Rice
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PDF similar to GB/T 39917-2021


Standard similar to GB/T 39917-2021

GB/T 29371.2   GB/T 29371.1   GB/T 29371.3   GB 20464   GB/T 39914   

Basic data

Standard ID GB/T 39917-2021 (GB/T39917-2021)
Description (Translated English) Variety genuineness and purity testing of main crops with SSR markers - Rice
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B21
Word Count Estimation 38,347
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 39917-2021: Variety genuineness and purity testing of main crops with SSR markers - Rice

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Variety genuineness and purity testing of main crops with SSR markers - Rice ICS 65.020.20 B21 National Standards of People's Republic of China SSR molecules for authenticity and purity of main crop varieties Marker detection rice Released on 2021-04-30 2021-11-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Table of contents

Foreword Ⅲ 1 Scope 1 2 Normative references 1 3 Terms, definitions and abbreviations 1 3.1 Terms and definitions 1 3.2 Abbreviations 2 4 General 2 5 Detection plan 2 5.1 General 2 5.2 Primer 3 5.3 Detection platform 3 5.4 Sample 3 5.5 Test condition 4 6 Equipment, reagents and solution preparation 4 6.1 Instruments and equipment 4 6.2 Reagent 5 6.3 Solution preparation 5 7 Authenticity test procedure 5 7.1 Primer synthesis 5 7.2 DNA extraction 8 7.3 PCR amplification 9 7.4 Separation of amplified products 10 7.5 Data analysis 11 8 Variety purity testing procedures 13 8.1 DNA extraction 13 8.2 Primer screening and synthesis 13 8.3 PCR amplification 14 8.4 Amplification product separation 14 8.5 Data analysis 14 9 Calculation and presentation of results 14 9.1 Verification of authenticity 14 9.2 Determination of purity 14 10 Results report 15 10.1 Verification of authenticity 15 10.2 Purity determination 15 Appendix A (Normative Appendix) Solution Preparation 16 Appendix B (informative appendix) Allele amplified fragment information 19 Table 1 Authenticity identification primer 5 Table 2 PCR amplification reaction system 9 Table 3 Purity determination candidate primer 13 Table B.1 Information on amplified fragments of major alleles of known varieties 19

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This standard is under the jurisdiction of the National Crop Seed Standardization Technical Committee (SAC/TC37). Drafting organizations of this standard. National Agricultural Technology Extension Service Center, China National Rice Research Institute, Ministry of Agriculture, Crop Seed Quality Supervision and Inspection Testing Center (Shenzhen), China Seed Group Co., Ltd. The main drafters of this standard. Xu Qun, Zhi Juzhen, Wei Xinghua, Liu Fengze, Jin Fang, Deng Hanchao, Dong Guoxing. SSR molecules for authenticity and purity of main crop varieties Marker detection rice

1 Scope

This standard specifies the detection principles, detection schemes, and SSR molecular markers for the authenticity and purity of rice (Oryzasativa L.) varieties. Testing procedures and results report. This standard applies to the authenticity verification and authenticity identification of rice varieties, and does not apply to substantive derived varieties (EDV) and genetically modified products. Identification of species. This standard applies to the determination of the purity of conventional rice varieties, hybrids and their parents.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 3543.1 General rules for inspection of crop seeds GB/T 3543.2 Crop Seed Inspection Regulation Sample GB/T 6682 Analytical laboratory water specifications and test methods 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Variety verification Compared with the standard sample of the corresponding variety name, the test confirms whether the labeling of the variety name of the sample for inspection is worthy of the name. 3.1.2 Variety identification After SSR molecular marker detection and through the SSR fingerprint data comparison platform (3.1.4) of the approved varieties, the screening and comparison are carried out to determine the authenticity of the samples for inspection. The name of the actual variety. 3.1.3 Standard sample The physical seed samples that have been recognized as representative of the characteristics of the approved variety and kept by the designated agency of the state. 3.1.4 SSR fingerprint data comparison platform SSRfingerprintblastplatform The standardized method of SSR molecular markers is used to detect the alleles of the variety standard samples, and the computer database technology and A search and comparison carrier for molecular data information of approved varieties constructed by network information technology. 3.1.5 Reference sample referencecontrolsample It is used to calibrate the sample whose SSR allele has defined the size of the amplified product fragment.

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