GB/T 29371.3-2012 PDF in English
GB/T 29371.3-2012 (GB/T29371.3-2012, GBT 29371.3-2012, GBT29371.3-2012)
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Technical rules for seed producing system of two-line hybrid rice -- Part 3: Technical rules for seed multiplication of photo/thermo-sensitive genic male sterile line
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Standards related to (historical): GB/T 29371.3-2012
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GB/T 29371.3-2012: PDF in English (GBT 29371.3-2012) GB/T 29371.3-2012
GB
ICS 65. 020. 20
B 21
NATIONAL STANDARD
OF THE PEOPLE’S REPUBLIC OF CHINA
Technical rules for seed producing system of two-line hybrid
rice - Part 3. Technical rules for seed multiplication of
photo/thermo-sensitive genic male sterile line
ISSUED ON. DECEMBER 31, 2012
IMPLEMENTED ON. JULY 1, 2013
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People's Republic of China;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Requirements of breeding base ... 4
3 Arrangement for breeding season ... 5
4 Sowing and seedling ... 6
5 Transplanting ... 7
6 Cultivation management ... 7
7 Cold water irrigation ... 8
8 Roguing and purifying ... 9
9 Reaping and sunning ... 10
Foreword
GB/T 29371 Technical rules for seed producing system of two-line hybrid rice consists of
five parts, as follows.
— Part 1. Terminology;
— Part 2. Technical rules for foundation seed production of photo/thermo-sensitive genic
male sterile line;
— Part 3. Technical rules for seed multiplication of photo/thermo-sensitive genic male
sterile line;
— Part 4. Technical rules for F1 seed production;
— Part 5. Technical rules for identification of variety purity & male sterility of MS line.
This is Part 3 of GB/T 29371.
This Part is drafted according to the rules specified in GB/T 1.1-2009.
This Part was proposed by Ministry of Agriculture of the People’s Republic of China.
This Part shall be under the jurisdiction of National Technical Committee (SAC/TC 37) on
Crop Seed of Standardization Administration of China.
The responsible drafting organizations. Hunan Hybrid Rice Research Center, Hunan
Longping Seed Industry Co., Ltd, Hunan Agricultural University, Hunan Seed
Administration, Anhui Academy of Agriculture, Jiangsu Academy of Agriculture, and
Guangdong Academy of Agriculture.
The chief drafting staffs of this standard. Zhou Chengshuo, Liu Aimin, Xiao Cenglin, Liu
Jianbing, Wang Shouhai, Lyu Chuangen, Wang Feng, Liao Cuimeng, Li Wengen, and
Ouyang Aihui.
Technical rules for seed producing system of two-line hybrid rice –
Part 3. Technical rules for seed multiplication of photo/thermo-sensitive
genic male sterile line
1 Scope
The part of the GB/T 29371 specifies the conditions and technical requirements required
in autumn breeding - for high-temperature sterile-type photo/thermo-sensitive genic male
sterile lines with cold irrigation breeding, Hainan breeding, and japonica photo-sensitive
genic male sterile lines.
The part is applicable to cold irrigation breeding and Hainan breeding for
high-temperature sterile-type photo/thermo-sensitive genic male sterile lines; and autumn
breeding and Hainan breeding for japonica rice photo-sensitive genic male sterile lines.
2 Requirements of breeding base
2.1 Insulation
Breeding field isolation spacing of indica shall be ≥200 m; for japonica rice, it shall be ≥300
m; or the time interval BETWEEN initial heading stage of other rice AND breeding male
sterile line in isolation area shall be ≥25d.
2.2 Quarantine
The breeding field is centralized and contiguous, with adequate sunlight, without the
same-family plant quarantine objects.
2.3 Cold irrigation breeding water source
With adequate water source, water temperature shall be ≤19°C. Capacity of reservoir
shall match with available breeding area.
— Capacity of reservoir is below 1×107 m3, and the available breeding area is below 3
hm2;
— Capacity of reservoir is 1×107 m3 - 2×107 m3, and the available breeding area is
3hm2 - 13 hm2;
— Capacity of reservoir is above 2×107 m3, and the available breeding area is 13 hm2
or above.
2.4 Cold irrigation breeding drainage facility standard
The irrigation drainage facility of cold irrigation breeding field shall be based on meeting
the inlet water temperature 19 - 20°C of breeding field; the outlet water temperature shall
lines
According to the sensitized characteristics of photo-sensitive genic male sterile lines and
sowing time at breeding place to determine the initial sowing period of sterile line, it shall
arrange the heading and flowering period of male sterile line to be before safe heading
period of local rice production.
4 Sowing and seedling
4.1 Requirement for seeds
4.1.1 Quality
The male sterile line seeds used for breeding shall be basic seeds, with purity of above
99.9%.
4.1.2 Sowing quantity
Male sterile line sowing quantity of early season rice and late season rice is 1.5 kg / 667
m2 - 2.0 kg / 667 m2. The sterile line sowing quantity of middle season rice is 1.0 kg / 667
m2 - 1. 5 kg / 667 m2. And sowing quantity of japonica photo-sensitive genic male sterile
lines is 2.0 kg / 667 m2 - 2.5 kg / 667 m2.
Adjust appropriately according to the level of germination rate, weight and size of
thousand-seeds, growth duration, and strength of tillering ability. For those with higher
germination rate, or lower-weight of thousand-seeds, or longer growth period, or stronger
tillering ability, the sowing quantity shall be reduced, otherwise it shall be increased.
4.2 Seed soaking for accelerating germination
For the same breeding base, unify the seed soaking for accelerating germination. Use
"Day soaking - night dew, multiple rise – multiple drop" method to soak seeds. It shall use
drug-liquid to soak the seeds for disinfection. Accelerate the germination timely after the
seeds absorb adequate water.
4.3 Sowing and seedling
4.3.1 Method of raising seedling
4.3.1.1 Moist seedling
The rice field is ditched and divided by plots. Flat the plot surface. Sow uniformly, with the
sowing quantity of 10 kg / 667 m2 - 15 kg / 667 m2, and step-valley after sowing. It shall
use thin film to cover, for the early spring sowing.
4.3.1.2 Two-stage seedling
The first stage is dry nursery seedling. Select adequate sunlight and flat dry land for seed
bed with the width of 1.2m. Firstly spread a layer of lime or plant ash; then lay abundant
6.1.1 Basal fertilizer
Fertilize 1,000 kg - 1,250 kg of decomposed field-yard manure and 8 kg - 10 kg in total
amount of pure N per 667 m2, 4 kg - 6 kg in total amount of P2O5, and 8 kg - 10 kg in total
amount of K2O, as the basal fertilizer.
6.1.2 Topdressing
5d-7d after transplanting, topdress 10 kg / 667 m2 - 12 kg / 667 m2 of urea, Apply fertilizer
depending on the growth of the seedlings. For cold irrigation breeding field, 3 d-6 d before
cold water irrigation, topdress 10 kg / 667 m2 of potassium chloride. Hainan winter
breeding should be fertilized in multiple times. The number and the amount of topdressing
are determined and depended on the field fertility and the condition of water and fertilizer.
6.2 Irrigating
For cold irrigation breeding field, in addition that it is irrigated by deep water when
conducting irrigation treatment, the rest period shall be conducted according to rice
planting management method.
6.3 Control and prevention of diseases and pests
It shall be operated by referring to the local control and prevention methods for the
same-season rice planting. For cold irrigation breeding field, it shall strength the control
and prevention for banded sclerotial blight and rice blast. For autumn breeding field, it
shall pay attention to the control and prevention for false smut and rice blast.
7 Cold water irrigation
7.1 Determination of beginning and ending of cold irrigation
7.1.1 Beginning of cold irrigation
When the groups young-ear differentiation indica-type reaches the end of stage IV
(differentiation period of male and female pistil); when the japonica type reaches the end
of stage III (differentiation period of spikelet primordia).
7.1.2 Ending of cold irrigation
When the groups panicle differentiation reaches the end of stage VI (meiosis stage).
7.2 Water temperature of cold irrigation field
Keep the water temperature at 2°C - 3°C lower than sterility starting temperature of
breeding male sterile lines.
7.3 Cold irrigation depth
Water depth shall be subject to completely submerge the young-ear, and gradually
Remove the single-plant whose particle shape, apiculus color, stigma color, plant height,
and plant shape are different from the standard sterile plant. And remove the single-plant
whose seed ripening rate of the whole-plant is obviously higher than the standard sterile
plant.
8.3 Field inspection
8.3.1 Full heading stage inspection
According to the breed typicality to inspect the uniformity of the group agronomic trait, and
according to the anther form and anther dehiscence powder situation to inspect the
consistency of the sterile starting point temperature, it is required that the mixed strain rate
of the field is ≤0.02%,
8.3.2 Inspection before the harvest
According to the breed typicality to re-inspect the uniformity of the group agronomic trait,
According to the single-plant’s ripening situation to inspect the consistency of the grou...
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Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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