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GB/T 39914-2021 English PDF

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GB/T 39914-2021: Variety genuineness and purity testing of main crops with SSR markers - Maize
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PDF similar to GB/T 39914-2021


Standard similar to GB/T 39914-2021

GB/T 29371.2   GB/T 29371.1   GB/T 29371.3   GB 20464   GB/T 39917   

Basic data

Standard ID GB/T 39914-2021 (GB/T39914-2021)
Description (Translated English) Variety genuineness and purity testing of main crops with SSR markers - Maize
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B21
Word Count Estimation 34,332
Issuing agency(ies) State Administration for Market Regulation, China National Standardization Administration

GB/T 39914-2021: Variety genuineness and purity testing of main crops with SSR markers - Maize

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Variety genuineness and purity testing of main crops with SSR markers - Maize ICS 65.020.20 B21 National Standards of People's Republic of China SSR molecules for authenticity and purity of main crop varieties Mark detection corn Released on 2021-04-30 2021-11-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Table of contents

Foreword Ⅲ 1 Scope 1 2 Normative references 1 3 Terms, definitions and abbreviations 1 3.1 Terms and definitions 1 3.2 Abbreviations 2 4 General 2 5 Detection plan 2 5.1 General 2 5.2 Primer 3 5.3 Detection platform 3 5.4 Sample 3 5.5 Test condition 4 6 Equipment, reagents and solution preparation 4 6.1 Instruments and equipment 4 6.2 Reagent 5 6.3 Solution preparation 5 7 Authenticity test procedure 5 7.1 Primer synthesis 5 7.2 DNA extraction 8 7.3 PCR amplification 8 7.4 Separation of amplified products 9 7.5 Data analysis 10 8 Variety purity testing procedures 11 8.1 DNA extraction 11 8.2 Primer screening and synthesis 11 8.3 PCR amplification 12 8.4 Separation of amplified products 12 8.5 Data analysis 13 9 Calculation and presentation of results 13 9.1 Verification of authenticity 13 9.2 Determination of purity 13 10 Results report 13 10.1 Verification of authenticity 13 10.2 Purity determination 14 Appendix A (Normative Appendix) Solution Preparation 15 Appendix B (informative appendix) Allele amplified fragment information 18 Table 1 Authenticity identification primer 5 Table 2 PCR amplification reaction system 8 Table 3 Purity determination candidate primer 12 Table B.1 Information on amplified fragments of major alleles of known varieties 18

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents. This standard was proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China. This standard is under the jurisdiction of the National Crop Seed Standardization Technical Committee (SAC/TC37). Drafting organizations of this standard. National Agricultural Technology Extension Service Center, Corn Research Center of Beijing Academy of Agriculture and Forestry Sciences, Beijing Seed Tube Management station. The main drafters of this standard. Wang Fengge, Zhi Juzhen, Yi Hongmei, Zhao Jianzong, Zhang Like, Tian Hongli, Lu Baochun. SSR molecules for authenticity and purity of main crop varieties Mark detection corn

1 Scope

This standard specifies the detection principles, detection schemes, and inspections of the SSR molecular markers for the authenticity and purity of maize (Zeamays L.) varieties. Test procedures and results report. This standard applies to the authenticity verification and authenticity identification of corn varieties, and does not apply to substantive derived varieties (EDV) and genetically modified varieties. Variety identification. This standard applies to the purity determination of maize single-cross varieties, and the purity determination of inbred lines, double-cross varieties and triple-cross varieties can be implemented by reference.

2 Normative references

The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 3543.1 General rules for inspection of crop seeds GB/T 3543.2 Crop Seed Inspection Regulation Sample GB/T 6682 Analytical laboratory water specifications and test methods 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Variety verification Compared with the standard sample of the corresponding variety name, the test confirms whether the labeling of the variety name of the sample for inspection is worthy of the name. 3.1.2 Variety identification After SSR molecular marker detection and through the SSR fingerprint data comparison platform (3.1.4) of the approved varieties, the screening and comparison are carried out to determine the authenticity of the samples for inspection. The name of the actual variety. 3.1.3 Standard sample The physical seed samples that have been recognized as representative of the characteristics of the approved variety and kept by the designated agency of the state. 3.1.4 SSR fingerprint data comparison platform SSRfingerprintblastplatform The standardized method of SSR molecular markers is used to detect the alleles of the variety standard samples, and the computer database technology and A search and comparison carrier for molecular data information of approved varieties constructed by network information technology. 3.1.5 Reference sample referencecontrolsample It is used to calibrate the sample whose SSR allele has defined the size of the amplified product fragment.

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