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GB/T 39101-2020: Determination of antimicrobial activity for polypeptides - Inhibition zone method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 39101-2020 | English | 189 |
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Determination of antimicrobial activity for polypeptides - Inhibition zone method
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GB/T 39101-2020
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Basic data | Standard ID | GB/T 39101-2020 (GB/T39101-2020) | | Description (Translated English) | Determination of antimicrobial activity for polypeptides - Inhibition zone method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,159 | | Date of Issue | 2020-09-29 | | Date of Implementation | 2021-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 39101-2020: Determination of antimicrobial activity for polypeptides - Inhibition zone method
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Determination of antimicrobial activity for polypeptides - Inhibition zone method
ICS 07.080
A40
National Standards of People's Republic of China
Inhibition zone method
2020-09-29 released
Implementation on 2021-04-01
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this standard. Beijing Technology and Business University, Hebei University of Science and Technology, Shenzhen Institute of Metrology and Quality Inspection, Hebei Agricultural University, Beijing
Sambo Technology Co., Ltd., Wuhan Mingliang Biotechnology Co., Ltd., Zhejiang Dongjie Biotechnology Co., Ltd., General Market Supervision and Management
Bureau of Food Evaluation Center, Institute of Biology, China Academy of Testing Technology.
The main drafters of this standard. Jia Yingmin, Ma Aijin, Wang Zhixin, Tian Yiling, Hao Shuai, Peng Hai, Zhou Lihua, Yang Zhijian, Zheng Yanyan, Zheng Gang,
Chen Jing, Jiang Yu, Yang Guowu.
Inhibition zone method
1 Scope
This standard specifies the method for determining the zone of inhibition for the antibacterial properties of peptides.
This standard applies to the determination of antibacterial properties and anti-filamentous fungi properties of polypeptides.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB 4789.2 National Food Safety Standard, Food Microbiological Examination, Determination of Total Colony
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Polypeptides
Between amino acids and proteins, a class of compounds formed by two or more amino acids connected by peptide bonds.
3.2
Inhibition zone
The zone of aseptic propagation at the boundary between the surface of the solid medium and the sample.
3.3
Antimicrobial activity
The ability to inhibit the reproduction of microorganisms is expressed by the antibacterial titer U value (AU).
4 Principle
After the polypeptide is in contact with the test indicator bacteria in the solid plate, the growth of the surrounding strains is inhibited, forming a sterile breeding area,
According to the diameter of the inhibition zone of the test bacteria on the surface of the solid plate, calculate the antibacterial titer U value to determine the activity.
5 Reagents or materials
Unless otherwise specified, the reagents used in this method are analytically pure, and the experimental water is the first grade water specified in GB/T 6682.
5.1 Normal saline
0.85% normal saline. Weigh 8.5g of sodium chloride in a volumetric flask, dissolve it with first-grade water and dilute to 1000mL. 121℃±1℃
Autoclave sterilization for 20 minutes.
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