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GB/T 39100-2020: Determination of antioxidant activity for polypeptides - DPPH and ABTS methods
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 39100-2020 | English | 189 |
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Determination of antioxidant activity for polypeptides - DPPH and ABTS methods
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GB/T 39100-2020
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Basic data | Standard ID | GB/T 39100-2020 (GB/T39100-2020) | | Description (Translated English) | Determination of antioxidant activity for polypeptides - DPPH and ABTS methods | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Classification of International Standard | 07.080 | | Word Count Estimation | 10,137 | | Date of Issue | 2020-09-29 | | Date of Implementation | 2021-04-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 39100-2020: Determination of antioxidant activity for polypeptides - DPPH and ABTS methods---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of antioxidant activity for polypeptides - DPPH and ABTS methods
ICS 07.080
A40
National Standards of People's Republic of China
Determination of Antioxidant Properties of Peptides by DPPH and ABTS Methods
2020-09-29 released
Implementation on 2021-04-01
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this standard. Hebei University of Science and Technology, Hebei Agricultural University, Beijing Technology and Business University, China Testing and Certification Group Co., Ltd.,
Beijing Sambo Technology Co., Ltd., Wuhan Mingliang Biotechnology Co., Ltd., Zhejiang Dongjie Biotechnology Co., Ltd., market supervision and management
Food Evaluation Center of General Administration of Science and Technology, China Academy of Inspection and Quarantine.
The main drafters of this standard. Wang Zhixin, Tian Yiling, Jia Yingmin, Ma Aijin, Hao Shuai, Peng Hai, Liu Wenqiu, Yang Zhijian, Zheng Gang, Jiang Yu,
Zou Mingqiang, Sun Yu, Qi Xiaohua.
Determination of Antioxidant Properties of Peptides by DPPH and ABTS Methods
1 Scope
This standard specifies the methods of DPPH and ABTS methods to determine the antioxidant properties of peptides.
This standard is applicable to the determination of the in vitro antioxidant properties of peptides.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Polypeptides
Between amino acids and proteins, a class of compounds formed by two or more amino acids connected by peptide bonds.
3.2
Antioxidantactivity
The ability to scavenge free radicals is expressed as the ratio (AO value) of the half-clearance of the tested polypeptide to the half-clearance of glutathione.
4 Abbreviations
The following abbreviations apply to this document.
ABTS. 2,2-Azino-bis(3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt [2,2'-azino-bis(3-ethylbenzothiazoline-6-
sulfonicacid)diammoniumsalt]
AO. antioxidantactivity
DPPH. 1,1-diphenyl-2-picrylhydrazyl (1,1-diphenyl-2-picrylhydrazyl)
DMSO. dimethylsulfoxide (dimethylsulfoxide)
EC50.half maximum clearance (halfmaximaleffectiveconcentration)
5 Reagents or materials
Unless otherwise specified, the reagents used in this method are analytically pure, and the test water is the first grade water specified in GB/T 6682.
5.1 Glutathione
L-reduced type, purity ≥98%.
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